A5015920248- Algal biology and biofuel production
- Photosynthetic Processes and Mechanisms
- Marine and coastal ecosystems
- Physiological and biochemical adaptations
- Microbial Community Ecology and Physiology
- Antioxidant Activity and Oxidative Stress
- Enzyme Catalysis and Immobilization
- Photoreceptor and optogenetics research
- Aquatic Ecosystems and Phytoplankton Dynamics
- Enzyme function and inhibition
- Protist diversity and phylogeny
- Diatoms and Algae Research
- Aquaculture Nutrition and Growth
- Biocrusts and Microbial Ecology
- CRISPR and Genetic Engineering
- Polar Research and Ecology
- Microbial Metabolic Engineering and Bioproduction
- Marine and coastal plant biology
- Light effects on plants
- Calcium Carbonate Crystallization and Inhibition
- Marine Bivalve and Aquaculture Studies
- Marine Toxins and Detection Methods
- Advanced Proteomics Techniques and Applications
- Electrochemical sensors and biosensors
- Marine Biology and Environmental Chemistry
Hanyang University
2016-2025
Korea Research Institute of Bioscience and Biotechnology
2023
Anyang University
2017-2021
Beth Israel Deaconess Medical Center
2009
Center for Vascular Biology Research
2009
Life University
2005-2008
Korea Institute of Ocean Science and Technology
2003-2004
University of California, Berkeley
2001-2003
Yonsei University
1998-2002
University of Minnesota
1994
Abstract Microalgae are versatile organisms capable of converting CO 2 , H O and sunlight into fuel chemicals for domestic industrial consumption. Thus, genetic modifications microalgae enhancing photosynthetic productivity biomass bio-products generation crucial both academic applications. However, targeted mutagenesis in with CRISPR-Cas9 is limited. Here we report, a one-step transformation Chlamydomonas reinhardtii by the DNA-free method rather than plasmids that encode Cas9 guide RNAs....
Haematococcus pluvialis cells predominantly remain in the macrozooid stage under favourable environmental conditions but are rapidly differentiated into haematocysts upon exposure to various stresses. Haematocysts characterized by massive accumulations of astaxanthin sequestered cytosolic oil globules. Lipidomic analyses revealed that synthesis storage lipid triacylglycerol (TAG) was substantially stimulated high irradiance. Simultaneously, remodelling membrane glycerolipids occurred as a...
Lutein and zeaxanthin are dietary carotenoids reported to be protective against age-related macular degeneration. Recently, the green alga Chlamydomonas reinhardtii has received attention as a photosynthetic cell factory, but potential of this for carotenoid production not yet been evaluated. In study, we selected C. CC-4349 strain best candidate among seven laboratory strains tested production. A knock-out mutant epoxidase gene induced by preassembled DNA-free CRISPR-Cas9 ribonucleoproteins...
Carbonic anhydrases convert CO2 to bicarbonate at a high turnover rate up 106 s–1, but their actual applications in conversion processes are hampered by poor stability. This study reports highly loaded and stabilized bovine carbonic anhydrase (bCA) upon being immobilized onto electrospun polymer nanofibers the form of enzyme precipitate coating (EPC). The EPC protocol, consisting covalent attachment, precipitation, cross-linking, maintained 65.3% initial activity even after incubated aqueous...
Abstract Microalgae can accumulate various carbon-neutral products, but their real-world applications are hindered by CO 2 susceptibility. Herein, the transcriptomic changes in a model microalga, Chlamydomonas reinhardtii , high-CO milieu (20%) evaluated. The primary toxicity mechanism consists of aberrantly low expression plasma membrane H + -ATPases (PMAs) accompanied intracellular acidification. Our results demonstrate that universally expressible PMA wild-type strains makes them capable...
Carotenoids are valuable pigments naturally occurring in all photosynthetic plants and microalgae as well selected fungi, bacteria, archaea. Green developed a complex carotenoid profile suitable for efficient light harvesting protection harbor great capacity production through the substantial power of endogenous 2-C-methyl-D-erythritol 4-phosphate (MEP) pathway. Previous works established successful genome editing induced significant changes cellular content Chlamydomonas reinhardtii. This...
The physiology of the unicellular green alga Dunaliella salina in response to abiotic stress has been studied for several decades. Early D. research focused on its remarkable salinity tolerance and ability, upon exposure various stresses, accumulate high concentrations -carotene other carotenoid pigments valued highly as nutraceuticals. simple life cycle growth requirements make this organism one large-scale commercially exploited microalgae natural carotenoids. Recent advances genomics...
ABSTRACT The halotolerant alga Dunaliella salina is a model for stress tolerance and used commercially production of beta-carotene (=pro-vitamin A). presented draft genome the genuine strain CCAP19/18 will allow investigations into metabolic processes involved in regulation responses, including carotenogenesis adaptations to life high-salinity environments.
Abstract Cryoprotectants such as antifreeze proteins (AFPs) and sugar molecules may provide a solution for icing problems. These anti-icing substances protect cells tissues from freezing by inhibiting ice formation. In this study, we developed method coating an industrial metal material (aluminum, Al) with AFP the Antarctic marine diatom, Chaetoceros neogracile ( Cn -AFP), to prevent or delay To coat Al -AFP, used Al-binding peptide (ABP) conjugator fused it -AFP. The ABP bound well did not...
Chlamydomonas reinhardtii is being transformed from a model organism to an industrial for the production of pigments, fatty acids, and pharmaceuticals. Genetic modification has been used increase economic value C. reinhardtii. However, low gene-editing efficiency position-effects hinder genetic improvement this microorganism. Recently, site-specific double-stranded DNA cleavage using CRISPR-Cas9 system applied regulate metabolic pathway in In study, we proved that gene expression can be...
The photosynthetic microorganism Chlamydomonas reinhardtii has been approved as generally recognized safe (GRAS) recently, this can excessively produce carotenoid pigments and fatty acids. Zeaxanthin epoxidase (ZEP), which converts zeaxanthin to violaxanthin, ADP-glucose pyrophosphorylase (AGP). These are key regulating genes for the xanthophyll starch pathways in C. respectively. In study, macular pigment-enriched microalgal oil, we attempted edit AGP gene an additional knock-out target zep...
A novel mutant (zea1) of the halotolerant unicellular green alga Dunaliella salina is impaired in zeaxanthin epoxidation reaction, thereby lacking a number beta-branch xanthophylls. HPLC analysis revealed that zea1 lacks neoxanthin (N), violaxanthin (V) and antheraxanthin (A) but constitutively accumulates (Z). Under low-light physiological growth conditions, (6 mg Z per g dry weight or 8 x 10(-16) mol Z/cell) had substantially higher content than wild type (0.2 0.5 Z/cell). Lack N, V, did...
In recent decades, harmful algal blooms (HABs) - commonly known as red tides have increasingly impacted human health, caused significant economic losses to fisheries and damaged coastal environments ecosystems. Here, we demonstrate a method control suppress HABs through selective lysis. The approach harnesses the algicidal effects of aminoclays, which are comprised high density primary amine groups covalently bonded by metal cation backbones. Positively charged colloidals aminoclays induce...
The sexual cycle of the unicellular Chlamydomonas reinhardtii culminates in formation diploid zygotes that differentiate into dormant spores eventually undergo meiosis. Mating between gametes induces rapid cell wall shedding via enzyme g-lysin; fusion is followed by heterodimerization sex-specific homeobox transcription factors, GSM1 and GSP1, initiation zygote-specific gene expression. To investigate genetic underpinnings zygote developmental pathway, we performed comparative transcriptome...