We describe a directed genome-engineering approach that combines genome-wide methods for mapping genes to traits [Warner JR, Reeder PJ, Karimpour-Fard A, Woodruff LBA, Gill RT (2010) Nat Biotechnol 28:856-862] with strategies rapidly creating combinatorial ribosomal binding site (RBS) mutation libraries containing billions of targeted modifications [Wang HH, et al. (2009) Nature 460:894-898]. This should prove broadly applicable various efforts focused on improving production fuels,...

Abstract Microalgae possess great potential as a source of sustainable energy, but the intrinsic inefficiency photosynthesis is major challenge to realize this potential. Photosynthetic organisms evolved phototaxis find optimal light condition for photosynthesis. Here we report microfluidic screening using competitive model alga, Chlamydomonas reinhardtii , rapid isolation strains with improved photosynthetic efficiencies. We demonstrated strong relationship between and efficiency by...

Recently, there has been emerging interest in various natural products with skin protective effects as they are recognized safe and efficient. Microalgae have developed chemical defense systems to protect themselves against oxidative stress caused by UV radiation producing bioactive compounds including a number of secondary metabolites, which potential for cosmeceutical applications. In addition, microalgae advantages sustainable source diverse functions due their rapid growth rate, high...

Abstract Microalgae – unicellular photosynthetic organisms have received increasing attention for their ability to biologically convert CO 2 into valuable products. The commercial use of microalgae requires screening strains improve the biomass productivity achieve a high-throughput. Here, we developed microfluidic method that uses magnetic field separate microdroplets containing different concentrations microalgal cells. separation efficiency is maximized using following parameters...

Abstract A problem with the use of Escherichia coli to produce foreign proteins is that although endogenously produced acetate physiologically indispensable, it inhibits protein expression. Here we firstly employed an antisense RNA strategy as elaborate metabolic engineering tool partially block biosynthesis two major pathway enzymes, phosphotransacetylase (PTA) and kinase (ACK). Three recombinant plasmids containing genes targeting either or both pta ackA were constructed, their effects on...

Hydrogenases catalyze reversible reaction between hydrogen (H2) and proton. Inactivation of hydrogenase by exposure to oxygen is a critical limitation in biohydrogen production since strict anaerobic conditions are required. While [FeFe]-hydrogenases irreversibly inactivated oxygen, it was known that [NiFe]-hydrogenases generally more tolerant oxygen. The physiological function [NiFe]-hydrogenase 1 still ambiguous. We herein investigated the H2 potential Escherichia coli vivo vitro. hyaA...

Abstract Background Solar energy is the ultimate source on Earth. The conversion of solar into fuels and sources can be an ideal solution to address problems. recent discovery proteorhodopsin in uncultured marine γ-proteobacteria has made it possible construct recombinant Escherichia coli with function light-driven proton pumps. Protons that translocate across membranes by generate a motive force for ATP synthesis ATPase. Excess protons also substrates hydrogen (H 2 ) production hydrogenase...

For economically viable biofuel production from microalgae, it is necessary to develop efficient analytical platforms for quantitative evaluation of different lipid productivities numerous microalgal species. Currently, culture, accumulation, and extraction depend on conventional benchtop methods requiring laborious time-consuming processes. A poly(dimethylsiloxane) (PDMS)-based integrated microfluidic platform was developed perform multiple steps in sample preparation a single device...

A droplet-based microfluidic system was applied for the rapid evaluation of photoautotrophic growth microalgae first time.

There is a growing interest in the photosynthetic carbon fixation by microalgae for production of valuable products from dioxide (CO2). Microalgae are capable transporting bicarbonate (HCO3 (-)), most abundant form inorganic species water, as source CO2 photosynthesis. Despite importance HCO3 (-) source, little known about chemotactic response to (-). Here, we showed chemotaxis model alga, Chlamydomonas reinhardtii, towards using an agarose gel-based microfluidic device with flow-free and...

For more than a decade, water-soluble, eco-friendly, biocompatible, and low-toxicity fluorescent nanomaterials have received considerable attention for their numerous in vivo vitro applications biomedical imaging, disease diagnostics, environmental monitoring. Owing to tunable photoluminescence properties, carbon-based luminescent shown great potential bioimaging, photocatalysis, biosensing among other applications. Marine environments provide excellent resources the fabrication of these...

Development of efficient culture and monitoring system for cell growth production useful materials is required practical utilization microalgae. In the present study, we developed a PDMS-based microreactor efficient, rapid microalgae growth, carotenoid content under diverse conditions. Due to advantages PDMS, optimized conditions (light intensity, pH, nitrate depletion, carbon dioxide concentration) improving rate astaxanthin productivity in considerably less time compared conventional...

We developed a PDMS-based multiplex microfluidic system with eight chambers and micropillar arrays to expedite multiple steps for lipid sample preparation from different microalgal strains.

Herein we report the solid-phase synthesis of a combinatorial aryl, alkyl-triazine library and its application to biofuel production. The combination Grignard reactions solid supported Suzuki coupling afforded unique 120 triazine compounds with high purities minimum purification steps. Through an unbiased phenotypic screening for improved generation in oleaginous yeast, found one diaryl derivative (E4) which increased biolipid production up 86%.

For several years, industrial damages caused by massive blooming and drifting of Sargassum horneri (S. horneri) called “golden tides” seaweeds have been continuously reported in Korea. National efforts made to produce useful cases application using the troublesome S. horneri. As a part that, CNDs–ZnO nanocomposite with antibacterial antifungal properties was synthesized through simple hydrothermal reaction horneri, results were verified this paper. The activities mainly determined disk...

Mussel adhesive proteins (MAPs) have been considered as potential underwater and medical bioadhesives. Previously, we reported a functional expression of recombinant MAP hybrid fp-151, which is fusion protein with six type 1 (fp-1) decapeptide repeats at each 5 (fp-5) terminus, practical properties in Escherichia coli. In the present work, introduced Vitreoscilla hemoglobin (VHb) co-expression strategy to enhance production levels fp-151 since VHb has successfully used for efficient oxygen...

The interest in ultraviolet (UV) radiation from the sun and artificial sources has been recently increasing due to its toxic effects, including sunburn, erythema, photodamage, photocarcinogenesis. In this study, we aimed develop a valuable nanomaterial that can block total UV rays using an undervalued, yet profitable marine organism. We successfully developed carbon nanodots (CNDs) CNDs-polyvinyl alcohol (PVA) composite film effectively protect against UV-A, UV-B, UV-C based on common brown...