A5043034237- Algal biology and biofuel production
- Photosynthetic Processes and Mechanisms
- Lipid metabolism and biosynthesis
- Astrophysical Phenomena and Observations
- Pulsars and Gravitational Waves Research
- Biocrusts and Microbial Ecology
- Gamma-ray bursts and supernovae
- Marine and coastal ecosystems
- Antioxidant Activity and Oxidative Stress
- Aquaculture disease management and microbiota
- Aquaculture Nutrition and Growth
- Microbial Metabolic Engineering and Bioproduction
- Astrophysics and Cosmic Phenomena
- Aquatic Ecosystems and Phytoplankton Dynamics
- Microbial Community Ecology and Physiology
- Reproductive biology and impacts on aquatic species
- Protist diversity and phylogeny
- Enzyme Catalysis and Immobilization
- Water Quality Monitoring and Analysis
- Spectroscopy Techniques in Biomedical and Chemical Research
- Inflammation biomarkers and pathways
- Astronomical Observations and Instrumentation
- Fatty Acid Research and Health
- 3D Printing in Biomedical Research
- High-pressure geophysics and materials
First Affiliated Hospital of Jinan University
2024
Chinese Academy of Sciences
2015-2023
Institute of Hydrobiology
2015-2023
Qingdao National Laboratory for Marine Science and Technology
2022
University of Chinese Academy of Sciences
2022
Institute of Hydrobiology, Biology Centre, Academy of Sciences of the Czech Republic
2021
State Development & Investment Corporation (China)
2021
Institute of High Energy Physics
2019-2020
Arizona State University
2010-2014
Williams (United States)
2012
Starch and neutral lipids are two major carbon storage compounds in many microalgae plants. Lipids more energy rich have often been used as food fuel feedstocks. Genetic engineering of the lipid biosynthesis pathway to overproduce has achieved only limited success. We hypothesize that through blocking competing produce starch, overproduction may be achieved. This hypothesis was tested using green microalga Chlamydomonas reinhardtii its low starch starchless mutants. discovered a dramatic...
To reveal the molecular mechanisms of oleaginousness in microalgae, transcriptomic and lipidomic dynamics oleaginous microalga Nannochloropsis oceanica IMET1 under nitrogen-replete (N+) N-depleted (N-) conditions were simultaneously tracked. At transcript level, enhanced triacylglycerol (TAG) synthesis N- primarily involved upregulation seven putative diacylglycerol acyltransferase (DGAT) genes downregulation six other DGAT genes, with a simultaneous elevation Kennedy pathway genes. Under...
Abstract Many unicellular microalgae produce large amounts (∼20 to 50% of cell dry weight) triacylglycerols (TAGs) under stress (e.g., nutrient starvation and high light), but the synthesis physiological role TAG are poorly understood. We present detailed genetic, biochemical, functional, analyses phospholipid:diacylglycerol acyltransferase (PDAT) in green microalga Chlamydomonas reinhardtii, which catalyzes via two pathways: transacylation diacylglycerol (DAG) with acyl groups from...
Oleaginous microalgae are promising feedstock for biofuels, yet the genetic diversity, origin and evolution of oleaginous traits remain largely unknown. Here we present a detailed phylogenomic analysis five Nannochloropsis species (a total six strains) one time-series transcriptome dataset triacylglycerol (TAG) synthesis on representative strain. Despite small genome sizes, high coding potential relative paucity mobile elements, genomes feature cores ca. 2,700 protein-coding genes large...
Major progress has been made in the past decade towards understanding of biosynthesis red carotenoid astaxanthin and its roles stress response while exploiting microalgae-based as a potent antioxidant for human health coloring agent aquaculture applications.In this review, astaxanthin-producing green microalgae are briefly summarized with Haematococcus pluvialis Chlorella zofingiensis recognized to be most popular astaxanthin-producers.Two distinct pathways synthesis along associated...
Summary Astaxanthin, a red ketocarotenoid with strong antioxidant activity and high commercial value, possesses important physiological functions in astaxanthin‐producing microalgae. The green microalga Haematococcus pluvialis accumulates up to 4% fatty acid‐esterified astaxanthin (by dry weight), is used as model species for exploring biosynthesis unicellular photosynthetic organisms. Although coordination of acid stoichiometric fashion was observed H. , the interaction mechanism unclear....
Haematococcus pluvialis cells predominantly remain in the macrozooid stage under favourable environmental conditions but are rapidly differentiated into haematocysts upon exposure to various stresses. Haematocysts characterized by massive accumulations of astaxanthin sequestered cytosolic oil globules. Lipidomic analyses revealed that synthesis storage lipid triacylglycerol (TAG) was substantially stimulated high irradiance. Simultaneously, remodelling membrane glycerolipids occurred as a...
Haematococcus pluvialis is a freshwater unicellular green microalga belonging to the class Chlorophyceae and of commercial interest for its ability accumulate massive amounts red ketocarotenoid astaxanthin (3,3′-dihydroxy-β,β-carotene-4,4′-dione). Using confocal Raman microscopy multivariate analysis, we demonstrate spectrally resolve resonance–enhanced signatures associated with β-carotene along chlorophyll fluorescence. By mathematically isolating these spectral signatures, in turn, it...
Raman-activated cell sorting (RACS) has attracted increasing interest, yet throughput remains one major factor limiting its broader application. Here we present an integrated droplet (RADS) microfluidic system for functional screening of live cells in a label-free and high-throughput manner, by employing AXT-synthetic industrial microalga Haematococcus pluvialis (H. pluvialis) as model. Raman microspectroscopy analysis individual is carried out prior to their microdroplet encapsulation,...
Summary Diacylglycerol acyltransferases ( DGAT s) catalyze a rate‐limiting step of triacylglycerol TAG ) biosynthesis in higher plants and yeast. The genome the green alga Chlamydomonas reinhardtii has multiple genes encoding type 2 s DGTT s). Here we present detailed functional biochemical analyses s. In vitro enzyme analysis using radiolabel‐free assay revealed distinct substrate specificities three s: Cr 1 preferred polyunsaturated acyl CoAs, monounsaturated 3 C16 CoAs. When...
Abstract Although production of biodiesels from microalgae is proved to be technically feasible, a commercially viable system has yet emerge. High‐cell‐density fermentation can coupled with photoautotrophic cultivation produce oils. In this study, by optimizing culturing conditions and employing sophisticated substrate feed control strategy, ultrahigh‐cell‐density 286 283.5 g/L was achieved for the unicellular alga Scenedesmus acuminatus grown in 7.5‐L bench‐scale 1,000‐L pilot‐scale...
We present the X-ray timing results of new black hole candidate (BHC) MAXI J1535-571 during its 2017 outburst from Hard Modulation Telescope (\emph{Insight}-HXMT) observations taken September 6 to 23. Following definitions given by \citet{Belloni2010}, we find that source exhibits state transitions Low/Hard (LHS) Intermediate (HIMS) and eventually Soft (SIMS). Quasi-periodic oscillations (QPOs) are found in intermediate states, which suggest different types QPOs. With large effective area...
ABSTRACT The unicellular green alga Haematococcus pluvialis has been exploited as a cell factory to produce the high‐value antioxidant astaxanthin for over two decades, due its superior ability synthesize under adverse culture conditions. However, slow vegetative growth favorable conditions and deterioration or death stress (e.g., high light, nitrogen starvation) limited production. In this study, new paradigm that integrated heterotrophic cultivation, acclimation of heterotrophically grown...
Abstract Heterotrophic cultivation of Chlorella has achieved commercial success, but the application biomass is still limited due to high cost production. In this study, an effective and industrially scalable heterotrphic technology been developed for a production strain sorokiniana GT‐1. Under optimized culturing conditions, ultrahigh concentration 271 247 g L −1 was in 7.5 bench‐scale 1000 pilot‐scale fermenters, respectively. Technoeconomic (TE) analysis indicated that C. GT‐1 could be...
Rapid, real-time and label-free measurement of the cellular contents biofuel molecules such as triacylglycerol (TAG) in populations at single-cell resolution are important for bioprocess control understanding population heterogeneity. Raman microspectroscopy can directly detect changes metabolite profile a cell thus potentially serve these purposes.Single-cell spectra (SCRS) unicellular oleaginous microalgae Nannochloropsis oceanica from cultures under nitrogen depletion (TAG-producing...
Chromochloris zofingiensis has obtained particular interest as a promising candidate for natural astaxanthin production. In this study, we established two-stage heterotrophic cultivation process, by using which both the growth of C. and accumulation are substantially enhanced. Specifically, ultrahigh biomass concentration 221.3 g L-1 was achieved under optimum culture conditions in 7.5 L fermenter during 12 days. When scaled-up 500 fermentor, yield reached 182.3 9 days, while content 0.068%...
The utilization of gibberellic acid-3, high carbon/nitrogen ratio and salinity concentration can effectively enhance astaxanthin biosynthesis in Chromochloris zofingiensis under the heterotrophic conditions, but underlying mechanisms remained yet to be investigated. metabolomics analysis revealed that enhancement glycolysis, pentose phosphate pathways (PPP), tricarboxylic acid (TCA) cycle led accumulation induction conditions. increased fatty acids significantly increase esterification....
The life cycle of the unicellular green alga Haematococcus pluvialis consists motile and nonmotile stages under typical growing conditions. In this study, we observed that cells were more susceptible than to high light, resulting in a decrease population density photo-bleaching. Using two strains, CCAP 34/12 (a cell dominated strain) SAG 34/1b strain), as model systems investigated cause death protective mechanisms survived light. light was attributed generation excess reactive oxygen...