A5101974383- Photosynthetic Processes and Mechanisms
- Algal biology and biofuel production
- Plant nutrient uptake and metabolism
- Lipid metabolism and biosynthesis
- Endoplasmic Reticulum Stress and Disease
- Plant Molecular Biology Research
- Plant Micronutrient Interactions and Effects
- Plant Reproductive Biology
- Cellular transport and secretion
- Biocrusts and Microbial Ecology
- Plant Stress Responses and Tolerance
- Fungal and yeast genetics research
Pohang University of Science and Technology
2012-2021
Fatty acids, the building blocks of biological lipids, are synthesized in plastids and then transported to endoplasmic reticulum (ER) for assimilation into specific lipid classes. The mechanism fatty acid transport from ER has not been identified. Here we report that AtABCA9, an ABC transporter Arabidopsis thaliana , mediates this transport. AtABCA9 was localized ER, atabca9 null mutations reduced seed triacylglycerol (TAG) content by 35% compared with WT. Developing seeds incorporated less...
Algal lipids are the focus of intensive research because they potential sources biodiesel. However, most algae produce neutral only under stress conditions. Here, we report that treatment with Brefeldin A (BFA), a chemical inducer ER stress, rapidly triggers lipid droplet (LD) formation in two different microalgal species, Chlamydomonas reinhardtii and Chlorella vulgaris. LD staining using Nile red revealed BFA-treated algal cells exhibited many more fluorescent bodies than control cells....
Endoplasmic reticulum (ER) stress is caused by the stress-induced accumulation of unfolded proteins in ER. Here, we identified and lipids that function downstream ER sensor INOSITOL-REQUIRING ENZYME1 (CrIRE1) contributes to tolerance Chlamydomonas (Chlamydomonas reinhardtii). Treatment with inducer tunicamycin resulted splicing a 32-nucleotide fragment basic leucine zipper 1 (bZIP1) transcription factor (CrbZIP1) mRNA CrIRE1 that, turn, loss transmembrane domain CrbZIP1, translocation...
Abstract Photosynthetic organisms are exposed to various environmental sources of oxidative stress. Land plants have diverse mechanisms withstand stress, but how microalgae do so remains unclear. Here, we characterized the Chlamydomonas reinhardtii basic leucine zipper (bZIP) transcription factor BLZ8, which is highly induced by Oxidative stress tolerance increased with increasing BLZ8 expression levels. regulated genes likely involved in carbon-concentrating mechanism (CCM): HIGH-LIGHT...
In many eukaryotes, endoplasmic reticulum (ER) stress activates the unfolded protein response (UPR) via transmembrane endoribonuclease IRE1 to maintain ER homeostasis. The in microalgae has not been studied detail. Here, we identified Chlamydomonas reinhardtii (CrIRE1) and characterized two independent knock-down alleles of this gene. CrIRE1 is similar IRE1s budding yeast, plants, humans, terms conserved domains, but differs having tandem zinc-finger domain at C terminus. was highly induced...
Concern about global warming has prompted an intense interest in developing economical methods of producing biofuels. Microalgae provide a promising platform for biofuel production, because they accumulate high levels lipids, and do not compete with food or feed sources. However, current algal oil involve subjecting the microalgae to stress conditions, such as nitrogen deprivation, are prohibitively expensive. Here, we report that fungicide fenpropimorph rapidly causes neutral lipids...