Abstract Xylem vessels function in the long-distance conduction of water land plants. The NAC transcription factor VASCULAR-RELATED NAC-DOMAIN7 (VND7) is a master regulator xylem vessel cell differentiation Arabidopsis (Arabidopsis thaliana). We previously isolated suppressor ectopic induced by VND7 (seiv) mutants. Here, we report that responsible genes for seiv3, seiv4, seiv6, and seiv9 are protein ubiquitination-related encoding PLANT U-BOX46 (PUB46), an uncharacterized F-BOX (FBX), PUB36,...

The increasing use of ionizing radiation (IR) in medical diagnosis and treatment has caused considerable concern regarding the effects occupational exposure on human health. Despite this concern, little information is available possible mechanism behind chronic low-dose irradiation. present study assessed potential genomic damage workers occupationally exposed to X-rays. A variety analyses were conducted, including assessing level DNA chromosomal aberrations (CA) as well cytokinesis-block...

Abstract The 26S proteasome is the major engine of protein degradation in all eukaryotic cells. Adenosine triphosphatase (ATPase) regulatory subunits (Rpts) are constituents that involved unfolding and translocation substrate proteins into core particle. In this study, by using brown planthopper Nilaparvata lugens as a model insect, we report biological importance Rpts female reproduction. We identified six homologous Rpt genes ( Rpt1 ‐ 6 ) N. . These were detected at high transcript levels...

The myeloid differentiation factor 2 (MD-2)-related lipid-recognition protein is involved in immune responses through recognizing bacteria lipopolysaccharide mammals, arthropods and plants. However, the physiological roles of MD-2 other biological processes are largely unknown. Here, we identified three homologue genes (NlML1, NlML2 NlML3) by searching genome transcriptome databases brown planthopper Nilaparvata lugens, a hemipteran insect species. Temporospatial analysis showed that NlML1...

The hydration of high-alkali red mud-based ferroaluminate cement (RCFA) clinker with calcium sulfate needs to be regulated. This study explored the effects type and dosage on properties RCFA clinker. research results show that when 4% gypsum was added, 3 d compressive strength 39.1 MPa, 28 63.2 MPa. increased by 61.6% compared strength. paste can adversely affected excessive anhydrite content. exothermic accelerated at beginning, but rate declined as process progressed. Sufficient sulfur...

Background Radon, a colorless and odorless radioactive gas, poses serious health risks. It is the second leading cause of lung cancer notably increases risk in smokers. Although previous epidemiological studies have mainly examined rates miners, effects radon on genomic stability its molecular mechanisms are not well understood. Methods This study evaluated chromosomal aberrations (CA) cytokinesis-block micronucleus (CBMN) miners’ lymphocytes, investigating relationship between cytogenetic...

Objective To compare the gene expression difference between 0.1 and 5 Gy X-ray irradiated cells,and to explore its possible mechanism.Methods A cDNA microarray corresponding 45033 human genes was used analyze transcriptional profiles of normal lymphoblastoid AHH-1 cells at 4 h after or irradiation.The with a fold change ≥ 2.0 were identified as differentially expressed genes.real-lime PCR Western blot confirm PERP.Results The assay showed that there 760 up-regulated 1222 down-regulated in...

Hankui Wu*, Zhiyong Xu, Ya Ma, He Qi, Jinxia Cao, Gang Li, Yong Chen, and Weihua Yang* College of Chemistry Chemical Engineering, Anyang Normal University, 455000, Henan Province, China. *E-mail: hkwu@aynu.edu.cn Institute Cotton Research, Chinese Academy Agriculture Sciences, Henan, cottontest@126.com (Received December 16, 2015; Accepted March 28, 2016)

Objective To explore the feasibility of using fluorescence in situ hybridization (FISH) technology to detect Calyculin A (CA)-induced prematurely condensed chromosome(PCC) after high-dose irradiation X-rays. Methods Human peripheral blood was irradiated by X-rays at 0, 1, 5, 10, 15 and 20 Gy. Whole cultured with RPMI 1640 medium, thereafter CA used induce premature chromosome condensation. The No.1 No.4 whole probe FISH, chromosomes were observed under microscope. aberration positive...

Objective To observe the radiosensitivity by targeting HIF-lα in human lung cancer and effects on tumor growth nude mice.Methods Radiosensitivity of A549 A549/HIF-1α ( － ) cells were tested clonogenic forming assay.A549/HIF-1 α( injected into male BALB/C mice.Tumor was observed.The expression HIF-1α microvessel density detected immunohistochemistry method.Results SERs gene silencing 1.03 normoxia 1.65 hypoxia.The sizes xenografts derived from A549/HIF-1α( significantly reduced compared to...

Objective To observe the consistency of T-cell receptor (TCR) genes mutation in lymphocytes rats after irradiation vivo and vitro.Methods A total 48 female were randomly divided into 6 equal groups.Peripheral blood samples from them collected to separate then irradiated X-ray with dose rate 200 cGy/min at doses 0,0.5,0.75,1.0,2.0,and 3.0 Gy,respectively.Then all lymphocyte cultured for 7 days.Flow cytometry direct immunofluorescence was used detect TCR gene mutation.The levels mutant...

Objective To investigate the chromosome damage in peripheral lymphocytes of underground gold miners.Methods Conventional method and cytokinesis-block micronuclens assay were used to analyze frequency aberrations micronucleus 58 miners,respectively.Results Frequencies chromosome-type aberrations,ehromatid-type total higher miners than those control group(0.72%,0.41%,1.16% vs 0.14%,0.18%,0.33,X2=44.322,9.501,50.476,P＜0.01).Both micronucleated cell rate group group(10.8‰ 11.6‰ 8.7‰...

objective To study the TCR gene mutation in peripheral blood lymphocytes induced by X-ray exposure using cultured cloning method.Methods Freshly isolated from healthy aduh donors were irradiated with doses ranging 0 to 8 Gy and interleukin2 phytohemagglutinin for 7 days.The mutant frequencies of gene(TCR MF)were detected flow cytonletry dose response curves fitted.Results MF increased going up.An aquadratic polynomial model was fitted.Conclusions could which serve as a potential biological...

Biological dosimetry indicators play important roles in the dose estimation of irradiated personnel nuclear accident and study radiation biological effects. However, chromosome aberration analysis cannot satisfy requirements mass rapid detection. Therefore, determining fast, simple, high-throughput biodosimetry has become a hot spot radiobiology. With development molecular biology technology theory, effects macromolecules have been studied extensively. The level is mainly focused on DNA...