Citrus is a kind of common fruit and contains multiple beneficial nutrients for human beings. Flavonoids, as class plant secondary metabolites, exist in citrus fruits abundantly. Due to their broad range pharmacological properties, flavonoids have gained increased attention. Accumulative vitro vivo studies indicate protective effects polymethoxyflavones (PMFs) against the occurrence cancer. PMFs inhibit carcinogenesis by mechanisms like blocking metastasis cascade, inhibition cancer cell...

Oxidative stress leads to various diseases, including diabetes, cardiovascular neurodegenerative and even cancer. The dietary flavonol glycoside, hyperoside (quercetin-3-O-galactoside), exerts health benefits by preventing oxidative damage. To further understand its antioxidative defence mechanisms, we systemically investigated the regulation of on damage induced hydrogen peroxide, carbon tetrachloride, cadmium in Saccharomyces cerevisiae. Hyperoside significantly increased cell viability,...

Unplanned exposure to radiation can cause side effects on high‐risk individuals; meanwhile, radiotherapies also injury normal cells and tissues surrounding the tumor. Besides direct damage, most of ionizing radiation‐ (IR‐) induced injuries were caused by generation reactive oxygen species (ROS). Human mesenchymal stem (hMSCs), which possess self‐renew multilineage differentiation capabilities, are a critical population participate in regeneration IR‐damaged tissues. Therefore, it is...

Managing and recycling electronic waste (e-waste), while useful necessary, has resulted in significant contamination of several environments China. The area around Tianjin, China become one the world's largest e-waste disposal centers, where electronics are processed by manually disassembly or burning, which can result serious exposure workers to a multitude toxicants.The present study assessed potential genomic damage involved e-waste.To detect cytogenetic DNA damage, chromosomal...

Hyperoside, an active compound found in plants of the genera Hypericum and Crataegus , is reported to exhibit antioxidant, anticancer, anti-inflammatory activities. Induction hepatic stellate cell (HSC) apoptosis recognized as a promising strategy for attenuation fibrosis. In this study, we investigated whether hyperoside treatment can exert antifibrotic effects human LX-2 cells. We that induced cells decreased levels α -smooth muscle actin ( -SMA), type I collagen, intracellular reactive...

The increasing use of ionizing radiation (IR) in medical diagnosis and treatment has caused considerable concern regarding the effects occupational exposure on human health. Despite this concern, little information is available possible mechanism behind chronic low-dose irradiation. present study assessed potential genomic damage workers occupationally exposed to X-rays. A variety analyses were conducted, including assessing level DNA chromosomal aberrations (CA) as well cytokinesis-block...

Abstract The purpose of this study is to evaluate the chemical composition, antioxidant and anti-bacterial activity cumin essential oils (CEOs) extracted by different techniques, including supercritical carbon dioxide extraction (SCE), subcritical butane (SBE) traditional solvent (SE). Our results indicated that CEOs are a valuable source bioactive compounds, aldehyde, γ-terpinene β-pinene. most abundant components found in obtained SCE SBE were similar, while SE, β-Cumic aldehyde (19.31%)...

Garcinol, a natural histone acetyltransferase inhibitor, has been reported to exhibit significant anti-proliferative activity in various cancer cell types. However, no information is available about the anti-cancer effects of garcinol on gallbladder carcinoma cells (GBC). In this study, GBC (GBC-SD and NOZ) were treated by subjected Cell Counting Kit-8 (CCK-8), GBC-SD selected for further transwell chamber assay, quantitative real-time polymerase chain reaction (qRT-PCR) Western blot...

Maintenance of genome integrity is critical for faithful propagation genetic information and the prevention mutagenesis induced by various DNA damage events. RecQ-mediated instability protein 1 (RMI1), together with Bloom syndrome topoisomerase IIIα, form an evolutionarily conserved complex that maintenance genomic stability. Herein, we report RMI1 depletion increases cell sensitivity to camptothecin treatment, as shown elevation genotoxic stress-induced double-strand breaks, a stronger...

RMI1 (RecQ-mediated genome instability protein 1) forms a conserved BTR complex with BLM, Topo IIIα, and RMI2, its absence causes instability. It has been revealed that localizes to nuclear foci BLM IIIα in response replication stress, functions downstream of promoting elongation. However, the precise during stress are not completely understood. Here we report knockdown cells hypersensitive hydroxyurea (HU). Using comet assay, show exhibit accumulation broken DNAs after being released from...

Ionizing radiation (IR) causes a wide variety of DNA lesions, which double-stranded breaks (DSBs) are the most deleterious. Homologous recombination (HR) is crucial route responsible for repairing DSBs. RecQ-mediated genome instability protein 1 (RMI1) member an evolutionarily conserved Bloom syndrome complex, prevents and resolves aberrant products during HR, thereby promoting stability. However, little known about role RMI1 in regulating cellular response to IR. This study aimed understand...

To investigate the mental state of medical imaging staff in Shandong Province, China, who have been on forefront COVID-19 epidemic during its late stage China. Questionnaires designed to assess anxiety and depression were administered on-location, 5331 complete results collected. SPSS software was used for statistical descriptions analysis. Rates disorders among workers 6.1% 6.5%, respectively, higher than those Chinese residents before epidemic. The outbreak Xinjiang, China; virus mutation...

The aim of this study was to measure occupational exposure doses technologists who dispense and inject radiopharmaceuticals in 7 positron emission tomography/computed tomography (PET/CT) departments. This done with the goal help improving protective designs PET departments and/or establishing national protection standards.Common LiF thermoluminescence dosimeters (TLDs) were placed on chest necklace monitor whole-body thyroid doses, respectively. Ring TLDs also worn both index fingers...

Background Radon, a colorless and odorless radioactive gas, poses serious health risks. It is the second leading cause of lung cancer notably increases risk in smokers. Although previous epidemiological studies have mainly examined rates miners, effects radon on genomic stability its molecular mechanisms are not well understood. Methods This study evaluated chromosomal aberrations (CA) cytokinesis-block micronucleus (CBMN) miners’ lymphocytes, investigating relationship between cytogenetic...

RecQ-mediated genome instability protein 1 (RMI1) is an important component of the BLM-Topo IIIα-RMI1-RMI2 complex and plays a critical role in maintaining stability. However, cellular functions RMI1 response to ionizing radiation (IR) are poorly understood. In this study, we found that knockdown led enhanced radiosensitivity apoptosis after irradiation. To analyze effect on expression circular RNAs (circRNAs), performed high-throughput RNA sequencing four groups human embryonic kidney (HEK)...

Objective: Although radiation workers are exposed to much lower doses of neutron-γ rays than those suffered in nuclear explosions and accidents, it does not mean that their health is affected by radiation. Lower do always cause morphological aberrations chromosomes, so more sophisticated tests must be sought specific alterations the cells. Our goal was characterize gene expression lymphocytes from logging who were continuously low We hypothesized combination cell type-specific transcriptomes...

Objective To study the biological effects of BLM-associated protein 75 (BLAP75) in ionizing radiation (IR) induced DNA damage response (DDR) . Methods BLAP75 was silenced cells by RNA interference. Single-cell gel electrophoresis performed to quantify breaks. Also siRNA-resistant BLAP75 expressed BLAP75-silenced rescue phenotype. Western blot used examine DDR phosphorylation upon IR. Results IR more breaks than 293T control without siRNA transfection. After expression cells, IR...

Interventional procedures (IPs) have been widely used to treat vascular anomalies (VA) in recent years. However, patients are exposed low-dose X-ray ionizing radiation (IR) during these fluoroscopy-guided IPs. We collected clinical information and IR doses IPs measured biomarkers including γ-H2AX, chromosome aberrations (CA), micronuclei (MN), which underpin radiation-induced DNA damage, from 74 pediatric before after For the children, range of dose-area product (DAP) values was 1.2 1754.6...

Objective To identify changes in the occurrence of endemic fluorosis order to provide scientific basis for making countermeasures. Methods Five villages from 14 counties mild, moderate and severe affected areas were selected by stratified cluster sampling every year whole province during 2006 - 2010. Water urinary fluorine determined ion selective electrode method(GB/T 8538-1995); dental children 8-12 years old was diagnosed with Dean method; skeletal according clinical indexing standards...