Effects of silencing BLAP75 on DNA damage induced by ionizing radiation

• DOI: 10.3760/cma.j.issn.1673-4114.2016.04.001
• Publication Date: 2016-07-25
• Authors: Chang Xu; Lianying Fang; Yangyang Kong; Lu Wang; Liqing Du; Yan Wang; Qin Wang
• Keywords: HEK 293 cells

Objective To study the biological effects of BLM-associated protein 75 (BLAP75) in ionizing radiation (IR) induced DNA damage response (DDR) .

Methods BLAP75 was silenced cells by RNA interference. Single-cell gel electrophoresis performed to quantify breaks. Also siRNA-resistant BLAP75 expressed BLAP75-silenced rescue phenotype. Western blot used examine DDR phosphorylation upon IR.

Results IR more breaks than 293T control without siRNA transfection. After expression cells, IR were similar cells. In IR, level Chk2 higher cells.

Conclusion BLAP75 alleviates IR-induced and possibly plays an important role repair.

Key words: Gene silencing; BLAP75; Radiation, ionizing; RNA interference