A5030066976- RNA and protein synthesis mechanisms
- Genomics and Phylogenetic Studies
- Plant Molecular Biology Research
- RNA modifications and cancer
- Gene expression and cancer classification
- Plant Reproductive Biology
- RNA Research and Splicing
- Physics of Superconductivity and Magnetism
- Lung Cancer Treatments and Mutations
- Plant Gene Expression Analysis
- Bioinformatics and Genomic Networks
- Machine Learning in Bioinformatics
- Antibiotics Pharmacokinetics and Efficacy
- Transportation Planning and Optimization
- Transportation and Mobility Innovations
- Cancer therapeutics and mechanisms
- Microbial Metabolic Engineering and Bioproduction
- Acute Myeloid Leukemia Research
- Cancer Treatment and Pharmacology
- Magnetic properties of thin films
- Pneumonia and Respiratory Infections
- Respiratory and Cough-Related Research
- Magnetic confinement fusion research
- Microbial Metabolism and Applications
- Lung Cancer Research Studies
Chiba University
2013-2022
Kanazawa University
2018-2022
Kanazawa Medical University
2018-2021
Greenwich University
2015
Chubu University
2008-2014
National Cancer Centre Japan
2013-2014
Hokkaido University
2012
Tokyo University of Science
2011
Nagoya University
2002-2008
Japan Society for the Promotion of Science
2006
Leaf primordia are generated at the periphery of shoot apex, developing into flat symmetric organs with adaxial-abaxial polarity, in which indeterminate state is repressed. Despite crucial role ASYMMETRIC LEAVES1 (AS1)-AS2 nuclear-protein complex leaf polarity specification, information on mechanisms controlling their downstream genes has remained elusive. We systematically analyzed transcripts by microarray and chromatin immunoprecipitation assays performed genetic rescue as1 as2 phenotypic...
Abstract Upstream open reading frames (uORFs) are often found in the 5′-leader regions of eukaryotic mRNAs and can negatively modulate translational efficiency downstream main ORF. Although effects most uORFs thought to be independent their encoded peptide sequences, certain control translation ORF a sequence-dependent manner. For genome-wide identification such regulatory uORFs, exhaustive searches for with conserved amino acid sequences have been conducted using bioinformatic analyses....
Abstract Motivation: Upstream open reading frames (uORFs) are often found in the 5′-untranslated regions of eukaryotic messenger RNAs. Some uORFs have been shown to encode functional peptides involved translational regulation downstream main ORFs. Comparative genomic approaches used genome-wide searches for encoding bioactive peptides, and by comparing uORF sequences between a few selected species or among small group species, with conserved amino acid (UCASs) identified plants, mammals...
Leaf primordia are generated around the shoot apical meristem. Mutation of ASYMMETRIC LEAVES2 (AS2) gene Arabidopsis thaliana results in defects repression meristematic and indeterminate state, establishment adaxial–abaxial polarity left–right symmetry leaves. AS2 represses transcription meristem-specific class 1 KNOX homeobox genes abaxial-determinant ETTIN/ARF3, KANADI2 YABBY5. To clarify role leaf polarity, we isolated mutations that enhanced associated with as2. We describe here...
An advanced public transportation system called the local initiative for neighborhood circulation (LINC) is proposed and realization of its usage investigated. LINC effectiveness efficiency comes from utilization a transit-station-oriented with elimination present bus stops. has three levels services. Level 1 regional trunk line service high-speed high-capacity provided by transit authority. 2 intercommunity express frequent reliable metro system. 3 could have many forms to serve within...
Control of mRNA half-life is a powerful strategy to adjust individual levels various stress conditions, because the degradation rate controls not only steady-state level but also transition speed levels. Here, we analyzed changes in response cold Arabidopsis cells using genome-wide analysis, which measurements and transcriptome analysis were combined. Half-lives average transcripts determined be elongated under conditions. Taking this general shift into account, identified more than thousand...
The Hha and YdgT proteins are suggested to modulate the expression of horizontally acquired genes by interacting with H-NS StpA, which play central roles in transcriptional silencing such genes. However, it is also possible that Hha/YdgT repress gene independently H-NS/StpA, as we have not fully understood molecular mechanism through H-NS/StpA activity. To gain further insight into basic functions Hha/YdgT, analysed impact hha/ydgT double inactivation on transcriptome profile Escherichia...
Abstract Patient‐derived xenograft ( PDX ) models are a useful tool in cancer biology research. However, the number of lung is limited. In present study, we successfully established 10 , including three adenocarcinoma AD ), six squamous cell carcinoma SQ and one large LA from 30 patients with non‐small NSCLC (18 2 mainly SCID hairless outbred SHO mice (Crlj: ‐Prkdc scid Hr hr ). Histology advanced clinical stage III ‐ IV status lymph node metastasis (N2‐3), maximum standardized uptake value...
Specific sequences of certain nascent peptides cause programmed ribosomal arrest during mRNA translation to control gene expression. In eukaryotes, most known regulatory are encoded by upstream open reading frames (uORFs) present in the 5'-untranslated region mRNAs. However, date, a limited number eukaryotic uORFs encoding have been reported. Here, we searched for peptide-encoding among Arabidopsis thaliana with evolutionarily conserved peptide sequences. Analysis vitro products 22...
ABSTRACT Although the DEAD-box RNA helicase family is ubiquitous in eukaryotes, its developmental role remains unelucidated. Here, we report that cooperative action between Arabidopsis nucleolar protein RH10, an ortholog of human DDX47, and epigenetic repressor complex ASYMMETRIC-LEAVES1 (AS1) AS2 (AS1-AS2) critical to repress abaxial (ventral) genes ETT/ARF3 ARF4, which leads adaxial (dorsal) development leaf primordia at shoot apices. Double mutations rh10-1 as2 (or as1) synergistically...
The diagnosis and treatment of soft tissue sarcomas (STS) have been difficult. Of the diverse histological subtypes, undifferentiated pleomorphic sarcoma (UPS) is particularly difficult to diagnose accurately, its classification per se still controversial. Recent advances in genomic technologies provide an excellent way address such problems. However, it often difficult, if not impossible, identify definitive disease-associated genes using genome-wide analysis alone, primarily because...
Abstract In this work, we aim to construct a new behavior analysis method by using machine learning. We used two cameras capture three-dimensional (3D) tracking data of zebrafish, which were analyzed fuzzy adaptive resonance theory (FuzzyART), type learning algorithm, identify specific behavioral features. The was tested based on an experiment in electric shocks delivered zebrafish and swimming tracked 3D simultaneously find shock-associated behaviors. By processing the obtained with...
The Arabidopsis ENHANCER OF SHOOT REGENERATION 1 (ESR1) is thought to be a key gene for commitment in vitro shoot regeneration tissue culture. ESR1 encodes member of the ETHYLENE RESPONSIVE FACTOR (ERF) family transcription factors. Here, we report identification genes downstream during regeneration. We previously demonstrated that protein functions as transcriptional activator; present study, upregulated after induction overexpression were screened by microarray experiments. Seven genes,...
We have demonstrated that an Arabidopsis serine/arginine rich-like protein, atSR45a, interacts with other splicing factors and its expression is markedly induced by high-light stress, suggesting the involvement of atSR45a in regulation stress-responsive alternative splicing. A whole-genome tiling array identified genes regulated comparing gene profiles wild-type knockout (KO-sr45a) plants under stress. The levels genomic regions within 217 were significantly altered KO-sr45a compared plants....
Arabidopsis ASYMMETRIC LEAVES2 (AS2) plays a critical role in leaf adaxial-abaxial partitioning by repressing expression of the abaxial-determining gene ETTIN/AUXIN RESPONSE FACTOR3 (ETT/ARF3). We previously reported that six CpG dinucleotides its exon 6 are thoroughly methylated METHYLTRASFERASE1, methylation levels inversely correlated with ETT/ARF3 transcript and at three out decreased as2-1. All these imply AS2 is involved epigenetic repression body DNA methylation. The mechanism AS2,...
Abstract Background Upstream open reading frames (uORFs) in the 5′-untranslated regions (5′-UTRs) of certain eukaryotic mRNAs encode evolutionarily conserved functional peptides, such as cis-acting regulatory peptides that control translation downstream main ORFs (mORFs). For genome-wide searches for uORFs with peptide sequences (CPuORFs), comparative genomic studies have been conducted, which uORF were compared between selected species. To increase chances identifying CPuORFs, we previously...
Abstract Background Recent advances in genome technologies have provided an excellent opportunity to determine the complete biological characteristics of neoplastic tissues, resulting improved diagnosis and selection treatment. To accomplish this objective, it is important establish a sophisticated algorithm that can deal with large quantities data such as gene expression profiles obtained by DNA microarray analysis. Results Previously, we developed projective adaptive resonance theory...
Abstract The levels and redox states of pyridine nucleotides, such as NADP(H), regulate the cellular homeostasis, which is crucial for photooxidative stress response in plants. However, how they are controlled poorly understood. An Arabidopsis Nudix hydrolase, AtNUDX19, was previously identified to have NADPH hydrolytic activity vitro , suggesting this enzyme be a regulator status. We herein examined physiological role AtNUDX19 using its loss-of-function mutants. were increased nudx19...