Australian Sweet Lupin (ASL) has a nutritional profile ideally suited for human consumption with high protein and fibre, but low starch fat content. The of germinated ASL may be better than ungerminated these improvements would provide further benefits in its use as an ingredient food applications. In this study the components such protein, crude flour following germination at 25℃ 90% - 95% relative humidity 9 days were determined. changes pattern during analysed using sodium dodecyl...

Background: Indonesia, home to the world’s largest Muslim population, enforces Law No. 33 of 2014, mandating halal certification for all products in country. However, meatballs, a popular Indonesian dish, frequently fall victim adulteration with non-halal meats, such as pork and wild boar due economic incentives. Aim: This study aims develop an analytical technique employing real-time polymerase chain reaction (PCR) specific primers detect meat within beef meatballs. Method: The research...

Summary Germinated Australian sweet lupin ( ASL ) flour (after germination for 7 days) was incorporated into muffin formulation at 0%, 2%, 4%, 6% and 8% of dried weight. The effect on the physical characteristics muffins including height, diameter, colour texture (hardness, cohesiveness, springiness chewiness) measured. stability phenolic, phytosterol compounds antiradical activity before after baking (190 °C, 25 min) were determined. level incorporation germinated affected volume, colour,...

Building loyalty is no longer an option in the banking world. In recent years a trend has emerged, namely tendency for customers to have more than one savings account at different banks. One of ways do this by marketing service quality and mix customer satisfaction. The Bank's compliance with government policies prevent spread COVID-19 Work From Home policy. Banking measures optimize employee efforts maintain health their closest people home while providing maximum protection from potential...

This study aims to determine the bioactivity of L.domesticum as cosmetic ingredient. The extraction was prepared by maceration with ethanol 96 % and ethyl acetate obtain ethanolic extract fruit flesh (BLE), peel (KLE), (KLE) (KLEA). Then, determination compounds conducted thin layer chromatography (TLC) analysed total phenolic flavonoid content colorimetric method. antioxidant capacity measured using radical scavenging assay 1,2-diphenyl-2-picrylhydrazyl (DPPH), β-carotene bleaching (BCB)...

Evaluation of phytoconstituents, nephro-protective and antioxidant activities Clitoria ternateaK.Sarumathy, M.S.Dhana Rajan, T.Vijay, J.Jayakanthi

Information about the Artocarpus altilis leaf as an antidiabetic associated with active compounds Gamma Amino Butyric Acid (GABA) is still limited. This study was conducted to determine effects of ethanolic extract A. leaves decoction and GABA on blood glucose levels insulin expression beta-cells in streptozotocin-induced diabetic rats.This done by using completely randomized design male Sprague Dewley rats. The rats were devided into normal control group groups. Levels bood measured strip...

The chemical contents and health benefits of black rice bran some cultivars have been investigated. However, there has little research on the ‘Cempo Ireng’ cultivar from Sleman, Yogyakarta. aim this present study was to determine anthocyanin, antioxidant activity, macro- micronutrients local cultivar. anthocyanin in extracted using maceration method with methanol as a solvent. extract obtained separated through preparative thin layer chromatography (TLC) silica GF254 mobile phase composed...

Ovomucoid (OVM) is one of the glycoproteins in egg white which heat resistant protein and to have protease inhibitor activity, that has potential as an anti-cancer. The aim this study was purify characterize ovomucoid from four types poultry eggs (commercial chickens, native ducks Muscovy ducks). We analyzed profile on sodium dodecyl sulphate-polyacrylamide gel electrophoresis (SDS-PAGE), secondary structure Fourier Transform Infra-Red (FTIR) Spectroscopy, amino acid profiles activity....

The adulteration practice in pharmaceutical industries, especially fats and oils used as a vehicle some products must be identified to assure its quality. In this study, Fourier Transform Infrared Spectroscopy (FTIR) combination with chemometrics techniques of multivariate calibration discriminant analysis (DA) were for the authentication extra virgin olive oil (EVOO) from rambutan seed fat (RSF). EVOO, RSF, mixture EVOO-RSF scanned using FTIR spectrophotometer at mid-infrared region...

Macaque monkey (Macaca fascicularis) meat (MM) has been reported to be consumed as meatball and soup products in Indonesia. MM is not allowed traded Indonesia considered halal meat; therefore, by Muslim communities. In this study, species-specific primer (SSP) targeting on mitochondrial displacement (D-loop) region coupled with real-time PCR assay was used identify beef meatballs. The product also subjected sequencing order ensure the adulteration practice of primers were designed silico...

Indonesia has a wide range of rice cultivars and pigments. This can be used as source ofphytochemical compounds for cancer prevention. research aims to analyze the cytotoxic activitiesof ethanolic extract black bran 4 local i.e. ‘Cempo Ireng’, ‘Woja Laka’, ‘Toraja’ and‘IR­64’ (white rice) on cells determine groups those extracts. First step,rice was extracted with ethanol. applied Raji (a human Burkitt Lymphomacancer), HepG2 liver cancer), Vero nonhuman cell line) in order measure...

Seledri ( Apium graviolens L.), kumis kucing Orthosiphon stamineus Benth.), and mengkudu Morinda citrifolia L.) have been used to treat hypertension diseases in Indonesia. Previous researches done determine antihypertensive activity of extracts the sample single extract. However, there are limited studies on effect a combination three samples. This research was therefore aimed study lowering blood tension normotensive spontaneously hypertensive rats. Non Invasive Blood Pressure technique...

The research aims to analyse the antioxidant, anti-tyrosinase and anti-wrinkle activities from grapefruit (Citrus Maxima L) strawberry extracts. Samples were extracted by maceration using 96% ethanol ethyl acetate, subsequently. Ferric Reducing Antioxidant Power (FRAP) β-carotene bleaching (BCB) used measure antioxidant activity. effect of was determined testing inhibition elastase collagenase enzyme, while activity analysed mushroom tyrosinase enzyme. results showed that extracts in...

Background and Objective: Dragon fruit (Hylocereus spp.), apple (Malus sylvestris Mill.) tomato (Solanum lycopersicum L.) are high potential sources of antioxidant compounds such as phenolics.The have the capability protecting cells tissues against free radicals.Secondary metabolite produced by callus cell culture from plant organs also acts a source antioxidants.This study aimed to determine optimal ratio sucrose 2,4-D in Murashige Skoog (MS) medium for induction different organ...

The aim of the study was determination glycaemic index (GI) local tubers such as Uwi ( Dioscorea alata ), Gadung hispida dan Talas Colocasia esculenta ). Tubers were prepared raw flour which administered orally to rats. Blood taken at 0; 0,5;  1 and 2 hour after administration. dose 2,5 kg/kg body weight (BW) whereas glucose a standart treatment. Area Under Curve (AUC) calculated plotting graph time versus levels. Glucose levels determined using GOD-PAP kit. GI values ratio AUC (0-2hr)...

The use of natural products has been widely used as a resource new bioactive chemical compounds. One them is the Paku Atai Merah (Angiopteris ferox Copel) tuber which long empirically by Dayak tribe East Kalimantan an anti-cancer. purpose this study was to determine anticancer cytotoxic activity extract and fractions against T47D breast cancer cells in vitro. Extract tubers obtained maceration method using ethanol solvent until ethanolic then fractionated various solvents obtain n-hexane,...

Introduction Cancer is a cellular disease caused by abnormal cell proliferation and it thus prohibits the normal functioning of organ in which these cells are located. can develop multicellular organisms due to range predisposing factors, including poor diet, unhealthy lifestyl

The grapefruit (Citrus maxima L.) is a plant known by the public as fruit consumed with various properties. This plant's use well known, such antioxidants, enhancing immunity, anti-aging, and antibacterial study aimed to identify analyze chemical compounds contained in C. rind. extract was obtained maceration method using ethanol ethyl acetate solvents. fractionation process carried out Column Chromatography. Observation of thin-layer chromatography profiles UV lamps 254 366 nm. Analysis...

Red dragon fruit (Hylocereus polyrhizus) and carrot (Daucus carota L.) have been known for their phenolic flavonoid compounds with antioxidants properties. Antioxidant activities including β-carotene bleaching (BCB) Cupric ion Reducing Capacity (CUPRAC) total content (TPC), (TFC), cytoprotective effect of the red extracts investigated in this study. Various concentrations (25-150 µg/mL) (25-200 were applied on fibroblast primary cell which has previously treated 1000 μM hydrogen peroxide...

Carica papaya L. leave was known containing Ribosome-inactivating proteins (RIPs), which demonstrated to have an in vitro cytotoxic effect on cancer cell lines. This researh examined the of protein fraction RIPs isolated from expressions p53 and Bcl-2, regulator apoptosis process, breast lines (T47D). leaves were by amonium sulfat precipitation. analyzed activity cleaving supercoiled double stranded DNA, order identify presence RIP. The active then tested citotoxic followed analysing bcl2...

Plant callus extracts are potential to be developed as ingredient in skincare products. Tomato is supposed contain protein-derivatives and or other components such secondary metabolites that play a role skin regeneration. Therefore the production of calli important studied for sustainable supply. This research aims obtain optimum medium induction analyze tomato development anatomically. In vitro culture response was assessed plant (Solanum lycopersicum L. ‘Permata’) induction. Seeds were...

Previous studies showed that Leunca herb ethanolic extract (LHE) has cytotoxic activity in several cancer cell lines such as HepG2 and HT-29. The also demonstrated potential agent to be developed co-chemotherapeutic combination with doxorubicin cisplatin. is supposed reduce occurance of resistance toxicity towards normal cell. In order verify safety before being applied human, toxicological genotoxic evaluation the important done. This research was therefore aimed investigate acute LHE....