[Main components from cultivated and wild Nardostachyos Radix et Rhizoma by LC-MS and GC-MS].

• DOI: 10.19540/j.cnki.cjcmm.20221210.101
• Publication Date: 2023-03-01
• Authors: Ying Li; Hai-Rong Zhong; Ri-Luo Feng; Tao Wang; Luo J; Zhengming Yang; Chen Chen; Xin-Jia Yan; Xiao-Ming Bao; Wenbin Li; Yuan Liu
• Keywords: Chlorogenic Acid; Radix (gastropod)

In this study, ultra-performance liquid chromatography-quadrupole/time-of-flight mass spectrometry(UPLC-Q-TOF-MS) and gas chromatography-mass spectrometry(GC-MS) were combined with non-targeted metabonomic analysis based on multivariate statistics analysis, the content of five indicative components in nardosinone was determined compared by UPLC. The main chemical Nardostachyos Radix et Rhizoma imitative wild cultivation comprehensively analyzed. results statistical spectrometry(LC-MS) GC-MS consistent. G1 G2 group G8-G19 clustered into category 1, while G7 G3-G6 2. After removing outlier data G1, G2, G7, one category, other category. Twenty-six identified according to positive negative ion modes detected LC-MS. components(VIP>1.5) using UPLC, revealing that chlorogenic acid, isochlorogenic acid A, C, linarin, nardosinone, total 1.85, 1.52, 1.26, 0.90, 2.93, 2.56 times those group, respectively. OPLS-DA obtained 10 diffe-rential peaks. Among them, relative α-humulene aristolene extremely significantly(P<0.01) significantly(P<0.05) higher than 7 such as 5,6-epoxy-3-hydroxy-7-megastigmen-9-one, γ-eudesmol, juniper camphor 12-isopropyl-1,5,9-trimethyl-4,8,13-cyclotetrade-catriene-1,3-diol lower Therefore, basically same. However, non-volatile some volatile opposite. This study provides scientific for comprehensive evaluation quality Rhizoma.