[Exploration of pharmacodynamic substances and potential mechanisms of Huazhuo Sanjie Chubi Decoction in treatment of gouty arthritis based on UPLC-Q-Exactive Orbitrap-MS technology and network pharmacology].

• DOI: 10.19540/j.cnki.cjcmm.20241025.201
• Publication Date: 2025-01-01
• Authors: Yan Xiao; Ting Zhang; Yingjie Zhang; Bin Huang; Peng Chen; Xiaohua Chen; Mingqing Huang; Xueting Chen; Youxin Su; Jiemei Guo
• Keywords: Decoction; Pharmacodynamics

Based on ultra-high performance liquid chromatography-quadrupole-Exactive Orbitrap mass spectrometry(UPLC-Q-Exactive Orbitrap-MS) technology and network pharmacology, this study explored the pharmacodynamic substances potential mechanisms of Huazhuo Sanjie Chubi Decoction in treatment gouty arthritis(GA). UPLC-Q-Exactive Orbitrap-MS was used to identify components Decoction, qualitative analysis its active ingredients carried out, with a total 184 identified. A 897 ingredient targets were screened through PharmMapper database, 491 GA-related disease obtained from OMIM, GeneCards, CTD databases. After Venn analysis, 60 intersecting obtained. The component target-GA target constructed Cytoscape platform, STRING database construct protein-protein interaction network, 16 core screened. subjected Gene Ontology(GO) Kyoto Encyclopedia Genes Genomes(KEGG) pathway enrichment analyses, component-target-pathway constructed. It found that main formula for GA phenols, flavonoids, alkaloids, terpenoids, key SRC, MMP3, MMP9, REN, ALB, IGF1R, PPARG, MAPK1, HPRT1, CASP1. Through GO it mainly involved biological processes such as lipid response, bacterial biostimulus response. KEGG showed pathways related included lipids atherosclerosis, neutrophil extracellular traps(NETs), IL-17, so on. In summary, terpenoids may be GA, mechanism other targets, well NETs, pathways.