FOXC2 is overexpressed and participates in cell proliferation in ovarian cancer

• DOI: 10.21037/5490
• Publication Date: 2015-10-22
• Authors: Yuan Quan; Zhe Quan; Chunhua Zheng; Weiguo Deng; Fanhui Meng; Yan Fu

Background: Forkhead box protein C2 (FOXC2) is an epithelial-to-mesenchymal transition (EMT)—inducing transcription factor and involved in several cancer developments. In this study, we aimed to investigate the role of FOXC2 ovarian cancer. Methods: A total 13 tissues (11 cases epithelial cancer, one case borderline serous cystadenoma normal tissue) were collected tissue specimens stained detect expression location by immunohistochemistry. Then shRNAs targeting mRNA designed construct FOXC2-shRNA-expressing lentiviral vector (LV- FOXC2). Real-time RT-PCR western blot assays conducted examine interference effects shRNA FOXC2. Additionally, cell counting kit-8 (CCK8) was performed proliferation ID8 cells (mouse cell) infected with FOXC2-shRNA-lentivirus. Results: No found a large number positive including vascular endothelial cells, mainly seen nucleus cells. Interference lentivirus successfully constructed virus titers 1.2×10 8 ~1.3×10 Tu/mL, which significantly decreased levels (P<0.05). Furthermore, suppressed time-dependent effect Conclusions: This study might help understand molecular mechanism provide theoretical foundation for development diagnosis treatment