Effect of methylene blue pretreatment on acute kidney injury induced by sepsis in rats

• DOI: 10.3760/cma.j.issn.0254-1416.2014.02.032
• Publication Date: 2014-02-20
• Authors: Mingxing Fang; Shiming Dong
• Keywords: Blood urea nitrogen; Methylene blue

Objective To evaluate the effect of methylene blue pertreatment on acute kidney injury induced by sepsis in rats.Methods Ninety male Sprague-Dawley rats,aged 1.5-2.5 months,weighing 200-250 g,were randomly assigned into 3 groups (n =30 each) using a random number table:sham operation group (S group),cecal ligation and puncture (CLP) (MB group).The rats were anesthetized with 10％ chloral hydrate 350 mg/kg.Normal saline 0.8 ml was injected via caudal vein CLP groups,while 15 mg/kg (in normal ml) MB.Sepsis after end administration MB groups.Twenty animals each chosen observed for 72 h survival rate.Ten sacrificed at 18 specimens removed microscopic examination determination expression poly (ADP-ribose) polymerase (PARP-1) immunohistochemistry Western blot.Blood samples taken from heart serum concentrations blood urea nitrogen (BUN),creatinine (Cr),cystatin C neutrophil gelatinase-associated lipocalin (NGAL).Results Compared S,the rate significantly decreased 24,48 operation,and BUN,Cr,cystatin NGAL PARP-1 tissues increased (P ＜ 0.05).Compared CLP,the 24 48 0.05).The pathological changes attenuated as compared CLP.Conclusion Methylene can attenuate through down-regulating PARP-1.

Key words: Methylene blue; Sepsis; Kindey