Comparison of effects of allogeneic blood transfusion and hemodiluted autotransfusion on cellular immune function in patients undergoing cesarean section

• DOI: 10.3760/cma.j.issn.0254-1416.2016.02.020
• Publication Date: 2016-02-20
• Authors: Xinhua Huang; Yaqin Zhang; Huaqi Yao; Zhu Huili
• Keywords: Autotransfusion; Fresh frozen plasma

Objective To compare the effects of allogeneic blood transfusion and hemodiluted autotransfusion on cellular immune function in patients undergoing cesarean section.

Methods Sixty patients, aged 20-35 yr, weighing 50-80 kg, American Society Anesthesiologists physical statusⅠ or Ⅱ, scheduled for elective section, were randomly divided into 2 groups(n = 30 each)using a random number table: group(group HAT)and ABT). Both groups received epidural anesthesia.The radial artery was cannulated after anesthesia, autologous collected group HAT.The timing: intraoperative loss expected to exceed 20% volume.For whom not volume, bleeding stopped, infused intravenously peritoneum closure.The when hemoglobin 1.5 times normal value, fresh frozen plasma 100-400 ml intravenously; platelet count<50×109/L, platelets 10-40 U intravenously.When entering operating room(T0), 1st day operation(T1), 5th operation(T2), samples detect levels T lymphocyte subsets CD3+ , CD4+ CD8+ (using FACScan flow cytometer), serum interleukin-2(IL-2)and IL-6 concentrations(by enzyme-linked immunosorbent assay). /CD8+ ratio calculated.

Results Compared with values at T0, ratio, IL-2 concentrations significantly decreased, increased T1, ABT, T1 HAT(P<0.05). Compared levels, HAT(P<0.05).

Conclusion Compared transfusion, has less inhibitory effect section.

Key words: Blood autologous; Blood component transfusion; Cesarean section; Immunity,