Changes in acetylation of histone in spinal dorsal horn in a rat model of persistent postoperative pain

• DOI: 10.3760/cma.j.issn.0254-1416.2016.03.026
• Publication Date: 2016-03-20
• Authors: Lijuan Song; Xingguo Hu; Li-Cai Zhang
• Keywords: Histone H4

Objective To investigate the changes in acetylation of histone spinal dorsal horn a rat model persistent postoperative pain.

Methods Ninety-six male Sprague-Dawley rats, weighing 200-250 g, aged 6-8 weeks, were randomly divided into 2 groups (n=48 each) using random number table: sham operation group (group S) and pain PPP). The evoked by skin/muscle incision retraction was established according to method described Flatters.After rats anesthetized with intraperitoneal chloral hydrate, skin superficial muscle medial thigh incised retractors inserted.This tissue retracted for 1 h. mechanical paw withdrawal threshold (MWT) measured at day before 1, 3, 7, 14, 21 days after operation.Four animals sacrificed each measurement MWT time point detection acetylated H3 (Ac-H3) H4 (Ac-H4) expression (by Western blot analysis) Ac-H3 Ac-H4 positive cells cord immunofluorescence histochemistry).

Results Compared S, significantly decreased 14 operation, Ac-H4b down-regulated 7 PPP (P<0.05 or 0.01). MWT, Ac-H4b, higher than (P<0.05).

Conclusion Acetylation is which may be involved development maintenance rats.

Key words: Histones; Acetylation; Pain, postoperative; Spinal