Prokaryotic expression and pilot application of capsid proteins of WU polyomavirus

• DOI: 10.3760/cma.j.issn.0254-5101.2011.06.013
• Publication Date: 2011-06-30
• Authors: Yinhui Zhang; Qiong Wang; Xue Jia; Lie Huang; Jian Liu; Xue-dong Lu
• Keywords: Antigenicity

Objective To express the capsid proteins of WU polyomavirus(WUPyV) for research and find antigen diagnostic value. Methods Coding sequences polyomavirus by PCR were cloned in prokaryotic expression vector PGEX-20T. Recombinant plasmids transformed into E. coli BL21(DE3) induced IPTG expression. identified Western blot. Results SDS-PAGE proved that recombinant showed three bands with molecular relative mass 69×103, 63×103 56×103. The recognized anti-GST McAb. antigenicity was tested blot using 16 positive 70 negative sera. Conclusion VP1, VP2 VP3 expressed can combine WUPyV-Ab have good antigenicity. They be used further research.

Key words: WU polyomavirus; Capsid protein; Prokaryotic