Fluorescent staining for the rapid diagnosis of rare invasive fungal infections

• DOI: 10.3760/cma.j.issn.0254-5101.2019.07.009
• Publication Date: 2019-07-31
• Authors: Feng Zhao; Sai Qiao; Junli Zhang; Ying Fu
• Keywords: Mucorales; Rhizopus arrhizus; Fluorescent staining; Gram staining; Rhizopus

Objective To evaluate the performance of an enhanced fluorescent staining for rapid diagnosis invasive mycosis, especially rare cases, considering traditional culture method always leads to delays in clinical its time consuming.

Methods Cases mycosis identified by our hospital from September, 2017 2018 were retrospectively analyzed. Three infections reported this study. Clinical specimens pretreated using standard procedures and then smeared on slides along with dye. Species pathogens according their morphology under microscope. The was used as a identify pathogenic species based colony morphology, followed PCR sequencing analysis further confirmation.

Results Three cases caused Talaromyces marneffei, Mucorales Prototheca rapidly diagnosed method. Sequencing results indicated Rhizopus arrhizus wickerhamii.

Conclusions Fluorescent is rapid, economic direct mycosis. fungi clear easy after fluorescence staining, which could be indicative highly suspected serve important complement method, or uncultured fungal pathogens.

Key words: Fluorescent staining; Rare fungus; Rapid