Porcine adipose derived stem cells: isolation, characterization and multi-differentiation potential

• DOI: 10.3760/cma.j.issn.1001-9030.2011.07.031
• Publication Date: 2011-07-08
• Authors: Baocheng Zhao; Bo Zhao; Zhenjun Wang
• Keywords: CD90; Aggrecan

Objective To investigate the isolation, culture, biological characteristics and multi-differentiation of porcine adipose-derived stem cells (ADSCs) in vitro. Methods ADSCs were obtained from back subcutaneous adipose tissue by collagenase digestion cultured Flow cytometric analysis cyto-immumofluorescence staining technique carried out to detect immunophenotypes ADSCs. Reverse transcription-polymerase chain reaction (RT-PCR) specific used evaluate multipotential differentiation phenotype Results The isolated positive for CD44 (90.2%) CD105 (99.2%), lacking CD34 (0.3%) CD45 (1.2%). After culture mesenchymal cell adipogenic, chondrogenic osteogenic basal media respectively 3, 4 3 weeks, oil red O (93%) alizarin bordeaux (94%), also expression peroxisome proliferator activated receptor (PPAR)-γ, collagen aggrecan was detected differentiated cells. Conclusion can differentiate towards adipocytes, chondrocytes osteoblasts when certain medium, likely served as one most optimal autogenous sources engineering.

Key words: Porcine; Adipose derived cells; Immunophenotype; Induce