Ultrasound-mediated microbubbles destruction enhances the transplantation of endothelial progenitor cells for improvement of chronic allograft vasculopathy in rats

• DOI: 10.3760/cma.j.issn.1004-4477.2016.01.017
• Publication Date: 2016-01-25
• Authors: Ji Ma; Suya Ma; Xianxian Yuan; Fang Ye
• Keywords: Endothelial progenitor cell

Objective To investigate the feasibility of using ultrasound-mediated destruction microbubbles (US+ MB) to enhance transplantation endothelial progenitor cells(EPCs) confer chronic allograft vasculopathy (CAV).

Methods Bone marrow derived mononuclear cells were isolated and induced in vitro. The abdominal aorta was performed. Four groups divided: control group without treatment (group A), injection with saline B), EPCs C), D MB+ EPCs) injected US applied MB prior infusion. All rats killed during 8 weeks after enable histological examination; SDF-1α expression detected by immunohistochemistry, TNF-α grafted aortas RT-PCR.

Results When transplantation, there a significant improvement aortic intima Group compared B C, respectively (P<0.05). In addition, significantly increased reduced aortas.

Conclusions US-mediated into can improves effectiveness repair delay progress CAV.

Key words: Sonication; Microbubbles; Endothelial cells; Chronic