A preliminary study on the method of single micro extrusion-based 3D printing of skin tissue

• DOI: 10.3760/cma.j.issn.1005-054x.2020.01.008
• Publication Date: 2020-02-10
• Authors: Hao Su; Guangzhe Jin; Yingying Le; Jihui Ju; Guangliang Zhang; Ping Zhang; Linfeng Tang; Ruixing Hou
• Keywords: HaCaT; Epidermis (zoology); Human skin; Dermal fibroblast; Skin equivalent

Objective To explore the feasibility of single micro extrusion 3D printing method for skin tissue.

Methods Fibroblasts were isolated and cultured from human dermis identified by immunofluorescence staining. Fibroblasts HaCaT cells used as seed cells. Gelatin, hyaluronic acid fibrinogen to form scaffolds. HE staining observe cell distribution printed skin. The viability in tissue was detected living/dead kit. Toluidine blue penetration assay detect barrier function skin.

Results More than 98% fibroblasts vimentin positive. When concentration epidermis 5 mg/ml 1 respectively, (4×106 cells/ml) 8 layers, (5×106 layer, prepared air-liquid interface 7 days detection showed that well stratified with thickness being (63.5±3.5) μm. (90.2±0.9)% (95.3±0.8)%, after submerge culture 2 days. After days, had similar normal skin.

Conclusion We have established a tissue, successfully tissue-engineered structure, which may become an effective mass production future.

Key words: Skin; Fibroblasts; HaCaT cells; Micro printing; Tissue structure