A comparison of two methods for determining glycated hemoglobin

• DOI: 10.3760/cma.j.issn.1007-1245.2013.01.007
• Publication Date: 2013-01-01
• Authors: Wen Zeng; Li Zeng; Yanqiang Hao; Liuqing Chen; Ju-fei Lian
• Keywords: Repeatability; Turbidimetry; Glycated hemoglobin; Linearity

Objective To explore the correlation and clinical application of turbidimetric inhibition immunoassay (TINIA) ion-exchange high-performance liquid chromatography (HPLC) for detecting glycosylated hemoglobin (HbAlC).Methods According to criteria on NCCLS,experiments precision,linearity,method comparison,and bias were conducted.The results analyzed by regression equation t test.Results The concentration HbAlc at a range 3.9％-15.2％ had good repeatability linearity TINIA or HPLC.Bias analysis showed HPLC negative as was higher level,and positive lower level.The data in two groups no statistical significance method comparison (P＞0.05).The with result determination Y axis that X revealed y =0.7857x +1.264 r =0.89,being positively related.Conclusions Through methodological evaluation,these methods determining HbAlC precision linear meets requirement,with comparable results.HPLC,which has better repeatability,is more suitable monitoring treatment diabetes mellitus.

Key words: Glycated hemoglobin; Immunosuppressive Turbidimetry; Ion exchange high performance