Expression of very long chain fatty acids protein 6 in the peripheral blood mononuclear cells of patients with primary gouty arthritis

• DOI: 10.3760/cma.j.issn.1007-7480.2015.03.009
• Publication Date: 2015-03-15
• Authors: Mengjun Pu; Wenguang Xie; Yufeng Qing; Yan Xing; Shu-Yue Pan; Mengyun Zhang; Wangtai Dang; Lingqin Li; Chang Zhou; Ya Qiu

Objective To investigate the differential expression levels of ELOVL6 mRNA and protein in peripheral blood mononuclear cells (PBMCs) patients with GA control population, clarify relationship between gene pathological mechanisms disease.

Methods ① Two hundreds seventy-four GA(158 AGA, 116 NAGA) 112 healthy controls (HC) were enrolled into study. The clinical data primary gouty arthritis collected. PBMCs isolated from vitro. ② expressions measured by Real-time polymerase chain reaction (RT-PCR) Western blot (WB) PBMCs, differences 3 groups compared. Pearson's correlation analysis was used to evaluate index which detected. One-way variance (ANOVA)and for statistical conalysis.

Results ① level reduced group (0.001 6±0.000 6) (P<0.01), AGA 3±0.000 7)<NAGA (0.002 0±0.001 5) (P<0.01); consequently, also lower (P<0.01). Negative detected SR (P<0.05) group; positive TC, Insulin found (r=0.38, 0.187; P<0.05).

Conclusion Altered may take part pathogenesis through immune, inflammatory metabolic mechan-isms.

Key words: Arthritis, gouty; Insulin; Metabolic syndrome; Elongation very long fatty acids 6