Effects of systemic depletion of macrophage on rheumatoid arthritis in mouse

• DOI: 10.3760/cma.j.issn.1673-4394.2017.06.005
• Publication Date: 2017-11-05
• Authors: Qing Zhang; Wan Shen; Qiushi Wang; Xiuyan Sun; Chun Li; Yifen Cui
• Keywords: CD68; Cellular infiltration

Objective To explore the role of macrophage in development rheumatoid arthritis(RA) by systemic depletion macrophages with Clodronate liposomes(Clo-lip).

Methods The spleens mice was detected hematoxylin and eosin(HE) staining immunohistochemistry.30 BALB/c were randomly divided into three groups: RA-model established as control A group; RA modelmice PBS-lip B group Clo-lip C group, which administered lips.The appearance mouse pawswere observed degree arthritis recorded histological features infiltrating inflammatory cells.

Results Compared horseshoe-shaped nuclei, multi-core large cells, CD68 few determined HE staining(t=15.46, P<0.05) immunohistochemistry(t=14.70, P<0.05)in group.The score lower than that other two groups.The difference statistically significant(t=8.578, P<0.05). showed there no cell infiltration articular cavity clo-lip group.

Conclusions Systemic affects occurrence RA, indicating plays a key RA.

Key words: Rheumatoid arthritis; Macrophage; Clodronate liposomes; Mouse