Knock-out of androgen receptor gene based on CRISPR/Cas9: an experimental study

• DOI: 10.3760/cma.j.issn.1674-1927.2016.04.004
• Publication Date: 2016-08-15
• Authors: Funeng Jiang; Guanxing Chen; Yingke Liang; Minyao Jiang; Jian‐Heng Ye; Wenhua Liu; Yongding Wu
• Keywords: Gene knockout

Objective To investigate the knock-out of androgen receptor (AR) gene at cellular level, and to provide cell lines for study AR pathway in prostate diseases.

Methods The sgRNA target sequence was designed by online software. The cloned into PX330 vector verified sequencing. CRISPR-AR transfected T293 cells, DNA extracted. PCR, restriction enzyme digestion sequencing were used identify knockout efficiency.

Results Sequencing that plasmid successfully constructed. 293T transfection showed had a efficiency 95.3%.

Conclusion Based on CRISPR/Cas9, established can be knock out genes which further construction lines.

Key words: Receptor, androgen; Benign prostatic hyperplasia; Prostatic neoplasms; CRISPR/Cas9