A5100771831- Blood properties and coagulation
- Neuroscience and Neuropharmacology Research
- Advanced Drug Delivery Systems
- RNA modifications and cancer
- MicroRNA in disease regulation
- Cancer-related gene regulation
- Cellular Mechanics and Interactions
- Erythrocyte Function and Pathophysiology
- Tendon Structure and Treatment
- Receptor Mechanisms and Signaling
- ATP Synthase and ATPases Research
- Microtubule and mitosis dynamics
- Angiogenesis and VEGF in Cancer
- Cell Image Analysis Techniques
- Skin and Cellular Biology Research
- Advanced Neuroimaging Techniques and Applications
- bioluminescence and chemiluminescence research
- Platelet Disorders and Treatments
- Cellular transport and secretion
- Machine Learning and ELM
- Neuroscience and Neural Engineering
- Histone Deacetylase Inhibitors Research
- Immune cells in cancer
- Circular RNAs in diseases
- Muscle Physiology and Disorders
Zhongda Hospital Southeast University
2024
Guangzhou Medical University
2022-2023
Guangzhou Women and Children Medical Center
2023
Southeast University
2021
Henan University Huaihe Hospital and Huaihe Clinical Institute
2020
Hunan Institute of Technology
2018-2019
Taizhou First People's Hospital
2019
Hengyang Academy of Agricultural Sciences
2018
Shenzhen Bao'an District People's Hospital
2018
Jilin University
2016
Blood clots perform an essential mechanical task, yet the behavior of fibrin fibers, which form structural framework a clot, is largely unknown. By using combined atomic force-fluorescence microscopy, we determined elastic limit and extensibility individual fibers. Fibrin fibers can be strained 180% (2.8-fold extension) without sustaining permanent lengthening, they up to 525% (average 330%) before rupturing. This largest observed for protein The data imply that monomers must able undergo...
Skeletal muscle microtubules (MTs) form a nonclassic grid-like network, which has so far been documented in static images only. We have now observed and analyzed dynamics of GFP constructs MT Golgi markers single live fibers the whole mouse vivo. Using confocal, intravital, superresolution microscopy, we find that MTs are dynamic, growing at typical speed ∼9 µm/min, forming small bundles build durable network. also show elements, associated with MT-organizing center proteins γ-tubulin...
Shank3, which encodes a scaffolding protein at glutamatergic synapses, is genetic risk factor for autism. In this study, we examined the impact of Shank3 deficiency on NMDA-type glutamate receptor, key player in cognition and mental illnesses. We found that knockdown with small interfering RNA (siRNA) caused significant reduction NMDAR-mediated ionic or synaptic current, as well surface expression NR1 subunits, rat cortical cultures. The effect siRNA NMDAR currents was blocked by an actin...
We have designed and tested a new, inexpensive, easy-to-make easy-to-use calibration standard for atomic force microscopy (AFM) lateral measurements. This new simply consists of small glass fiber known dimensions Young’s modulus, which is fixed at one end to substrate can be bent laterally with the AFM tip other end. has equal or less error than commonly used method using beam mechanics determine cantilever’s constant. It transferable, thus providing universal tool comparing calibrations...
The cytoskeleton (microtubules, actin and intermediate filaments) has a cell type‐specific spatial organization that is essential reflects health. We are interested in understanding how changes the of microtubules contribute to muscle diseases such as Duchenne muscular dystrophy (DMD). grid‐like immunofluorescence microtubule pattern fast‐twitch fibers lends itself well visual assessment. more complicated other does not. Furthermore, assessment not quantitative. Therefore we have developed...
Skeletal muscle pathologies cause irregularities in the normally periodic organization of myofibrils. Objective grading morphology is necessary to assess health, compare biopsies, and evaluate treatments evolution disease. To facilitate such quantitation, we have developed a fast, sensitive, automatic imaging analysis software. It detects major minor morphological changes by combining texture features Fourier transform (FT) techniques. We apply this tool second harmonic generation (SHG)...
Background Because of their regenerative and paracrine abilities, cardiac stem cells (CSCs) are the most appropriate, optimal promising candidates for development medicine strategies. However, native exogenous CSCs in ischemic hearts exposed to various pro-apoptotic or cytotoxic factors preventing abilities. Methods Results We examined effects H2O2 on mouse (mCSCs), observed that hydrogen peroxide (H2O2) treatment induces mCSCs apoptosis via caspase 3 pathway, a dose-dependent manner. then...
The monoamine system in the prefrontal cortex has been implicated various mental disorders and major target of anxiolytics antidepressants. Clinical studies show that serotonin norepinephrine reuptake inhibitors (SNRIs) produce better therapeutic effects than single selective inhibitors, but underlying mechanisms are largely unknown. Here, we found low dose SNRIs, by acting on 5-HT(1A) α2-adrenergic receptors, synergistically reduced AMPA receptor (AMPAR)-mediated excitatory postsynaptic...
The abnormal expression of miRNAs is directly related to the development human diseases. Predicting potential candidate associated with diseases can contribute detection, diagnosis, treatment and prevention complex effective inference calculation method relationship between an supplement biological experiments. It great help in prevention, prognosis This paper proposes a novel information diffusion based on network consistency (IDNC) for identifying disease microRNAs. model first synthesizes...
Background: Tetralogy of Fallot (TOF) is the most common cyanotic heart disease. However, association cardiac metabolic reprogramming changes and underlying molecular mechanisms in TOF-related chronic myocardial hypoxia damage are still unclear. Methods: In this study, we combined microarray transcriptomics analysis with liquid chromatography tandem-mass spectrometry (LC–MS/MS) spectrum metabolomics to establish that occurs response damage. Two Gene Expression Omnibus (GEO) datasets,...
Alternative polyadenylation (APA) is a critical post-transcriptional process that generates mRNA isoforms with distinct 3' untranslated regions (3' UTRs), thereby regulating localization, stability, and translational efficiency. Cell-type-specific APA extensively shapes the diversity of cellular transcriptome, particularly during cell fate transition. Despite its recognized significance, precise regulatory mechanisms governing cell-type-specific remain unclear. In this study, we uncover...
Hypoxia-inducible factor-1alpha (HIF-1α) is a transcription factor implicated in physiological and pathological responses to hypoxia. The present study aims investigate the effect mechanism of HIF-1α on cardiopulmonary bypass (CPB)-related myocardial injury, thereby conferring theoretical basis for clinical treatment injury CPB.An experimental model CPB was established rats by surgery. Adenovirus-packaged overexpression vectors antiagomiRNA were used overexpress NR4A1 or inhibit miR-124-3p...
Background: Tumor classification is important for accurate diagnosis and personalized treatment has recently received great attention. Analysis of gene expression profile shown relevant biological significance thus become a research hotspot new challenge bio-data mining. In the methods, some algorithms can identify few genes but with time complexity, get small complex methods unsatisfactory accuracy, this article proposed extraction method profile. Methods: paper, we propose tumor subtypes...
Objective To investigate the knock-out of androgen receptor (AR) gene at cellular level, and to provide cell lines for study AR pathway in prostate diseases. Methods The sgRNA target sequence was designed by online software. The cloned into PX330 vector verified sequencing. CRISPR-AR transfected T293 cells, DNA extracted. PCR, restriction enzyme digestion sequencing were used identify knockout efficiency. Results Sequencing that plasmid successfully constructed. 293T...