A5034857903- Ion channel regulation and function
- Genetic Neurodegenerative Diseases
- Diet and metabolism studies
- Adipose Tissue and Metabolism
- Muscle Physiology and Disorders
- Mitochondrial Function and Pathology
- Cellular transport and secretion
- Biochemical effects in animals
- Inflammatory mediators and NSAID effects
- Osteoarthritis Treatment and Mechanisms
- Cardiovascular Effects of Exercise
- Microtubule and mitosis dynamics
- Gestational Diabetes Research and Management
- Streptococcal Infections and Treatments
- Ion Channels and Receptors
- Breastfeeding Practices and Influences
- Nuclear Structure and Function
- Cardiovascular Function and Risk Factors
- Pain Management and Placebo Effect
- Histone Deacetylase Inhibitors Research
- Fatty Acid Research and Health
- Cellular Mechanics and Interactions
- Protein Hydrolysis and Bioactive Peptides
- Attention Deficit Hyperactivity Disorder
- Child Nutrition and Feeding Issues
Laboratoire Dielen (France)
2021-2023
Aix-Marseille Université
2021
Centre National de la Recherche Scientifique
2021
Institut de Neurophysiopathologie
2021
National Institutes of Health
2013-2019
National Institute of Arthritis and Musculoskeletal and Skin Diseases
2013-2019
Office of Science
2013-2019
Grenoble Institute of Neurosciences
2009-2012
Inserm
2005-2012
Université Joseph Fourier
2007-2012
Skeletal muscle microtubules (MTs) form a nonclassic grid-like network, which has so far been documented in static images only. We have now observed and analyzed dynamics of GFP constructs MT Golgi markers single live fibers the whole mouse vivo. Using confocal, intravital, superresolution microscopy, we find that MTs are dynamic, growing at typical speed ∼9 µm/min, forming small bundles build durable network. also show elements, associated with MT-organizing center proteins γ-tubulin...
Triadin is a multiple proteins family, some isoforms being involved in muscle excitation-contraction coupling, and having still unknown functions. To obtain clues on triadin functions, we engineered knock-out mouse line characterized the physiological effect of ablation skeletal function. These mice presented reduced strength, which seemed not to alter their survival has been present work. We first checked these expression level different calcium homeostasis observed fast muscles an increase...
Articular cartilage experiences mechanical constraints leading to chondral defects that inevitably evolve into osteoarthritis (OA), because has poor intrinsic repair capacity. Although OA is an incurable degenerative disease, several dietary supplements may help improve outcomes. In this study, we investigated the effects of Dielen® hydrolyzed fish collagens from skin (Promerim®30 and Promerim®60) (Promerim®40) analyze phenotype metabolism equine articular chondrocytes (eACs) cultured as...
Irritable bowel syndrome (IBS), a multifactorial intestinal disorder, is often associated with disruption in permeability as well an increased expression of pro-inflammatory markers. The aim this study was to first test the impact treatment glutamine (Gln), food supplement containing natural curcumin extracts and polyunsaturated n-3 fatty acids (Cur); bioactive peptides from fish protein hydrolysate (Ga); probiotic mixture Bacillus coagulans, Lactobacillus acidophilus, gasseri helveticus....
To identify the function of triadin in skeletal muscle, adenovirus-mediated overexpression Trisk 95 or 51, two major muscle isoforms, was induced rat primary cultures, and physiological behavior modified cells analyzed. Overexpression did not modify expression level their protein partners ryanodine receptor, dihydropyridine other triadin. Caffeine-induced calcium release also unaffected by overexpression. Nevertheless, absence extracellular calcium, depolarization-induced almost abolished...
The terminal cisternae represent one of the functional domains skeletal muscle sarcoplasmic reticulum (SR). They are closely apposed to plasma membrane invaginations, T-tubules, with which they form structures called triads. In triads, physical interaction between T-tubule-anchored voltage-sensing channel DHPR and SR calcium RyR1 is essential because it allows depolarization-induced release that triggers contraction. This based on peculiar both T-tubules cisternae. However, little known...
The triadin isoforms Trisk 95 and 51 are both components of the skeletal muscle calcium release complex. To investigate specific role in physiology, we overexpressed or using adenovirus-mediated gene transfer newborn mice. Overexpression either alters fiber morphology, while leaving unchanged expression ryanodine receptor, dihydropyridine calsequestrin. We also observe an aberrant caveolin 3 95- 51-overexpressing muscles. Using a biochemical approach, demonstrate that is associated with...
Differentiated mammalian cells and tissues, such as skeletal muscle fibers, acquire an organization of Golgi complex microtubules profoundly different from that in proliferating still poorly understood. In adult rodent muscle, the multinucleated fibers have hundreds elements (GE), small stacks cisternae serve microtubule-organizing centers. We are interested role GE organizing a peculiar grid located fiber cortex, against sarcolemma. Modifications this mdx mouse model Duchenne muscular...
Anxiety is a high frequency disorder in the general population. It usually treated with benzodiazepines, which cause side effects and dependence that could make withdrawal difficult. Alternative treatments are therefore needed to reduce use of anxiolytics, particularly for adjustment anxiety. An observational, multicentre, prospective, longitudinal study has been conducted by practitioners one gynaecologist evaluate efficacy dietary supplement on anxiety (Stress 2 study). Patients diagnosed...
Cartilage is a non-innervated and non-vascularized tissue. It composed of one main cell type, the chondrocyte, which governs homeostasis within cartilage tissue, but has low metabolic activity. Articular undergoes substantial stresses that lead to chondral defects, inevitably osteoarthritis (OA) due intrinsic repair capacity cartilage. OA remains an incurable degenerative disease. In this context, several dietary supplements have shown promising results, notably in relief symptoms. study, we...
// Thomas Perez 1 , 2 Raphaël Bergès Hélène Maccario Sarah Oddoux and Stéphane Honoré Aix-Marseille University, CNRS, INP, Institute of NeuroPhysiopathology, Marseille, France APHM, Hôpital de la Timone, Service Pharmacie, Correspondence to: Honoré, email: stephane.honore@univ-amu.fr Keywords: vorinostat; glioblastoma; EB1; microtubule; tubulin Received: September 21, 2020 Accepted: February 01, 2021 Published: 16, Copyright: © et al. This is an open access article distributed under the...