Osteoporosis in patients with systemic lupus erythematosus (SLE) is thought to be the result of accelerated osteoclastogenesis induced by pro-inflammatory cytokines such as tumor necrosis factor (TNF). However, molecular mechanisms involved osteoblastogenesis SLE are not fully understood. We investigated bone morphogenetic protein-2 (BMP-2)-induced osteoblastic capacity marrow-derived mesenchymal stem cells (BMMSCs) from and TNF signaling system determining BMP-2-induced regulatory pathways....

// Lele Wu 1,* , Yuzhi Wang Yan Liu 1 Shiyi Yu Hao Xie Xingjuan Shi Sheng Qin 2 Fei Ma Tuan Zea Tan 3 Jean Paul Thiery 3,4,5 and Liming Chen The Key Laboratory of Developmental Genes Human Disease, Ministry Education, Institute Life Science, Southeast University, Nanjing, PR China for Comparative Genomics Bioinformatics & Jiangsu Biodiversity Biotechnology, College Nanjing Normal Cancer Science Institute, National University Singapore, 14 Medical Drive, Singapore 4 Molecular Cell Biology,...

The human immune system exhibits sexual dimorphism in autoimmune diseases such as systemic lupus erythematosus (SLE). Female sex hormones, including 17β-estradiol, are strongly implicated the gender bias SLE. CD40 is a costimulatory molecule and plays crucial role modulating response of effector cells. We have previously shown that 17β-estradiol up-regulated expression altered minichromosome maintenance protein 6 (MCM6) gene dendritic cells (DCs). mechanism correlation between MCM6 presence...

The aim of the present study was to investigate effect 7,8‑dihydroxyflavone (7,8‑DHF) against osteoarthritis (OA) and examine its regulatory role in nuclear factor (erythroid‑derived 2)‑like 2 (Nrf2) signaling pathway chondrocytes. Primary mouse chondrocytes were treated with 7,8‑DHF expression Nrf2 downstream heme oxygenase 1 (HO‑1). surgical destabilization medial meniscus model used assess effectiveness protecting cartilage from damage, knee harvested mice for histological analysis....

Postural control deficits are a potential cause of persistent and recurrent pain in patients with chronic low back (CLBP). Although some studies have confirmed that the dorsolateral prefrontal cortex (DLPFC) contributes to regulation CLBP, its role postural CLBP remains unclear. Therefore, this study aimed investigate DLPFC activation healthy controls under different upright stance task conditions.Twenty (26.50 ± 2.48 years) 20 (25.75 3.57 performed tasks three conditions: Task-1 was static...

The balance between cell proliferation and differentiation is essential for maintaining the neural progenitor pool brain development. Although mechanisms underlying at transcriptional level have been studied intensively, post-transcriptional regulation of remains largely unclear. Here, we show that deletion alternative splicing regulator PQBP1 in striatal progenitors results defective development due to impaired neurogenesis spiny projection neurons (SPNs). Pqbp1-deficient exhibit declined...

Backgrounds Recent explosion of biological data brings a great challenge for the traditional clustering algorithms. With increasing scale sets, much larger memory and longer runtime are required cluster identification problems. The affinity propagation algorithm outperforms many other classical algorithms is widely applied into researches. However, time space complexity become bottleneck when handling large-scale sets. Moreover, similarity matrix, whose constructing procedure takes long...

Purpose: Vaccines play important roles in antitumor biotherapy. Autophagy tumor cells plays a critical role depredating proteins, including tumor-specific antigens and tumor-associated antigens. We aimed to induce collect tumor-derived autophagosomes (DRibbles) from as novel vaccine by inhibiting the functions of proteasomes lysosomes. Materials methods: DRibbles were prepared their morphological autophagic properties characterized. Dendritic (DCs) generated bone marrow monocytes mice...

Cancer stem cells (CSCs) have been reported in a variety of cancers. SRY-box 2 (SOX2) is member the SOX family transcription factors and has shown to play critical role maintaining functions CSCs promoting tumor initiation. However, underlying mechanisms for transcriptional regulation SOX2 gene are unclear. In this study, using silico experimental approaches, we identified repressor GATA binding 1 (TRPS1), an atypical GATA-type factor, as regulator that represses expression thereby...

Backgrounds Despite continuing progress in X-ray crystallography and high-field NMR spectroscopy for determination of three-dimensional protein structures, the number unsolved newly discovered sequences grows much faster than that determined structures. Protein modeling methods can possibly bridge this huge sequence-structure gap with development computational science. A grand challenging problem is to predict structure from its primary (residues sequence) alone. However, predicting residue...

Synaptic scaling is an extensively studied form of homeostatic plasticity critically involved in various brain functions. Although it accepted that synaptic expressed through the postsynaptic accumulation AMPA receptors (AMPARs), induction mechanism remains elusive. In this study, we show TTX treatment induces rapid but transient release neurite growth-promoting factor 2 (NGPF2), and necessary sufficient for TTX-induced up. addition, inhibition anaplastic lymphoma kinase (ALK)-LIMK-cofilin...

The roles of the MYC transcription factor in transcriptional regulation have been studied intensively. However, general mechanism underlying recruitment to chromatin is less clear. Here, we found that Krüppel-like ZFP281 plays important recruiting active promoters mouse embryonic stem cells. At genome scale, broadly associated with MYC, and depletion significantly reduces levels RNA polymerase II at ZFP281- MYC-cobound genes. Specially, required for Lin28a oncogene pri-let-7 transcription....

Neutrophil gelatinase-associated lipocalin (NGAL) is a candidate diagnostic biomarker for acute kidney injury (AKI). Since there no specific treatment to reverse AKI, good such as NGAL can increase the performance of clinical care. Therefore, timely, and sensitive assay detecting critical determination. In this study, we established solid-phase proximity ligation detection using polyclonal antibodies conjugated with pair oligonucleotides. The data are read out Ct value via quantitative...

ABSTRACT The evolutionarily conserved C-terminal binding protein (CtBP) has been well characterized as a transcriptional co-repressor. Herein, we report previously unreported function for CtBP, showing that lowering CtBP dosage genetically suppresses Polycomb group (PcG) loss-of-function phenotypes while enhancing of trithorax (trxG) in Drosophila, suggesting the role gene activation is more pronounced fly development than thought. In cells, show required derepression most direct PcG target...

HIV/AIDS is the leading cause of death by infectious diseases in China, a country with largest population world. China has made great efforts and progress studying, treating, controlling past 15 years. This situation calls need for systematic analysis status capability global research. It will facilitate world to integrate utilize resources from fighting against disease. A bibliometric research was conducted period 2000-2014. articles citations were measured as productivity. set indices...

Protein secondary structure prediction is one of the most important and challenging problems in structural bioinformatics, which has been an essential task determining function proteins. Despite significant progress made recent years, protein maintains its status as prime unsolved computational biology. A novel probability revise table based hidden Markov model (PRT-HMM) method presented this paper with considering dependencies among state transitions. We initial predicted through looking up...

Background: TEA domain transcription factor 4 (TEAD4) is a member of the transcriptional enhancer (TEF) family factors, which studied to be linked tumorigenesis and progression various forms cancers, including lung adenocarcinoma (LUAD). However, specific function this gene in LUAD remains explored. Method: A total 19 genes related Hippo pathway were analyzed identify significant involved progression. The TCGA-LUAD data (n = 585) from public databases mined, differentially expressed (DEGs)...

Alternative polyadenylation (APA) is a critical post-transcriptional process that generates mRNA isoforms with distinct 3' untranslated regions (3' UTRs), thereby regulating localization, stability, and translational efficiency. Cell-type-specific APA extensively shapes the diversity of cellular transcriptome, particularly during cell fate transition. Despite its recognized significance, precise regulatory mechanisms governing cell-type-specific remain unclear. In this study, we uncover...

This paper provides the design and optimization of a knee rehabilitation exoskeleton robot, focusing on mechanical structure, material selection, motion mechanics analysis. First, it starts by identifying requirements based joint biomechanics. The uses tandem elastic actuator as main power source includes crank guide rod mechanism to adjust lengths thigh calf segments. helps reduce peak force demand during actuation improves comfort human-computer interaction degree lightweight device. study...