A5022429042- Neuroscience and Neuropharmacology Research
- Neurotransmitter Receptor Influence on Behavior
- Receptor Mechanisms and Signaling
- Memory and Neural Mechanisms
- Stress Responses and Cortisol
- Olfactory and Sensory Function Studies
- Neuroinflammation and Neurodegeneration Mechanisms
- Neuropeptides and Animal Physiology
- Proteoglycans and glycosaminoglycans research
- Neuroendocrine regulation and behavior
- Sleep and Wakefulness Research
- Neurogenesis and neuroplasticity mechanisms
- Circadian rhythm and melatonin
- Fibromyalgia and Chronic Fatigue Syndrome Research
- Animal testing and alternatives
- Zebrafish Biomedical Research Applications
- Photoreceptor and optogenetics research
- Neuroscience of respiration and sleep
- Adipose Tissue and Metabolism
- Regulation of Appetite and Obesity
- Epigenetics and DNA Methylation
- Genetic Neurodegenerative Diseases
- Neural dynamics and brain function
- Nerve injury and regeneration
- Retinoids in leukemia and cellular processes
Legacy Health
2019-2024
Washington State University
2008-2024
Washington State University Vancouver
2013-2024
Banner - University Medical Center Tucson
1997
University of California, Los Angeles
1987
UCLA Medical Center
1987
University of Ljubljana
1987
University of Maryland, College Park
1986
German Cancer Research Center
1981
Heidelberg University
1981
Behavioral sensitization to amphetamine-like psychostimulants is manifest as a progressive increase in drug-induced anxiety and paranoia which can culminate psychopathologies, such paranoid psychosis panic attacks. Sensitization may also mediate the facilitation of drug relapse addicts by increasing reinforcing value acute administration. The primary animal model for psychostimulant-induced psychopathologies involves repeated, non-contingent administration rodents, produce enduring...
Pyramidal neurons in the medial prefrontal cortex (mPFC) critically contribute to cocaine-seeking behavior humans and rodents. Activity of these is significantly modulated by GABAergic, parvalbumin-containing, fast-spiking interneurons, majority which are enveloped specialized structures extracellular matrix called perineuronal nets (PNNs), integral maintenance many types plasticity. Using a conditioned place preference (CPP) procedure, we found that removal PNNs primarily from prelimbic...
Sensitization to cocaine refers the behavioral model of addiction where motor stimulant effect is augmented for months after discontinuing a regimen repeated injections. There has been speculation that neuroadaptations mediating this sensitization phenomenon may, in part, underlie changes produced by chronic abuse, including paranoia, craving and relapse. Criteria are proposed may assist determining which most relevant regard. Using these criteria, presented endeavors incorporate issuing...
In the semisynthetic compound phorbol 12-retinoate 13-acetate (PRA), antipromoting principle of vitamin A acid is combined with structure a ester tumor promoter. skin NMRI mice, single topical application PRA induces inflammation, epidermal proliferation, and sustained hyperplasia to similar extent apparently along same pathway as an equimolar dose strong promoter 12-myristate (PMA). The mitogenic effects both PMA are mediated by prostaglandin E synthesis. However, in mouse initiated...
Locomotor sensitization is a common and robust behavioral alteration in rodents whereby following exposure to abused drugs such as cocaine, the animal becomes significantly more hyperactive response an acute drug challenge. Here, we further analyzed role of cocaine-induced silent synapses nucleus accumbens (NAc) shell their contribution development locomotor sensitization. Using combination viral vector-mediated genetic manipulations, biochemistry, electrophysiology paradigm with repeated,...
Male Sprague-Dawley rats that were naive or had been treated with five daily saline cocaine injections (15 mg/kg i.p.) subsequently challenged an injection of cocaine, and extracellular dopamine content in the medial prefrontal cortex (mPFC) was measured using vivo microdialysis. Cocaine challenge increased levels from base line all three groups rats, but augmentation significantly reduced cocaine-pretreated group, compared saline-pretreated group. In contrast, mPFC not different among after...
Drug-induced malfunction of nucleus accumbens (NAc) neurons underlies a key pathophysiology drug addiction. changes in intrinsic membrane excitability NAc are thought to be critical for producing behavioral alterations. Previous studies demonstrate that, after short-term (2 d) or long-term (21 withdrawal from noncontingent cocaine injection, the shell (NAcSh) is decreased, and decreased NAcSh increases acute locomotor response cocaine. However, animals exhibit distinct cellular alterations...
Persistent drug seeking/taking behavior involves the consolidation of memory. With each use, memory may be reactivated and reconsolidated to maintain original During reactivation, become labile susceptible disruption; thus, molecules involved in plasticity should influence acquisition and/or reconsolidation. Recently, matrix metalloproteinases (MMPs) have been shown neuronal plasticity, presumably by their regulation extracellular (ECM) synaptic reorganization during learning. We...
Recent research suggests that drug-related memories are reactivated after exposure to environmental cues and may undergo reconsolidation, a process can strengthen memories. Conversely, reconsolidation be disrupted by certain pharmacological agents such the drug-associated memory is weakened. Several studies have demonstrated disruption of using drug-induced conditioned place preference (CPP) task, but no explored whether cocaine-associated similarly in cocaine self-administering animals...
Abstract: Expression of the myelin proteolipid protein (PLP) was examined in nuclei and polysomes 12–27‐day‐old quaking, jimpy, shiverer mouse brains 2–27‐day‐oId normal compared with expression basic proteins (MBPs). Northern blots showed presence multiple PLP RNAs, developmental which coincided my‐elination. Two major 3.5 2.6 kilo‐bases length, were observed both cytoplasmic polyri‐bosomes nuclei, and, addition, a larger 4.6‐kilobase RNA nuclei. Quantitative measurements slot blot analyses...
Perineuronal nets (PNNs) are aggregations of extracellular matrix molecules that critical for plasticity. Their altered development or changes during adulthood appear to contribute a wide range diseases/disorders the brain. An increasing number studies examining contribution PNN various behaviors and types plasticity have analyzed fluorescence intensity Wisteria floribunda agglutinin (WFA) as an indirect measure maturity PNNs, with brighter WFA staining corresponding more mature dim immature...
<h3>Abstract</h3> We previously reported that perineuronal nets (PNNs) are required for cocaine-associated memories. Perineuronal extracellular matrix primarily surrounds parvalbumin (PV)-containing, GABAergic fast-spiking interneurons (FSIs) in the medial prefrontal cortex (mPFC). Here we measured impact of acute (1 d) or repeated (5 cocaine exposure on PNNs and PV cells within prelimbic infralimbic regions mPFC. Adult rats were exposed to 1 5 d stained (using <i>Wisteria floribunda</i>...
In vitro rotating disk electrode (RDE) voltammetry and in vivo microdialysis were used to characterize dopamine clearance the rat medial prefrontal cortex (mPFC). RDE studies indicate that inhibition by cocaine, specific inhibitors of transporter (DAT) norepinephrine (NET), low Na + produced a 50–70% decrease velocity clearance. Addition monoamine (MAO) inhibitors, l -deprenyl, clorgyline, pargyline, or nialamide 30–50% inhibition. Combined effects uptake with -deprenyl on additive (up 99%...
Levels of tyrosine hydroxylase (TH) immunoreactivity and mRNA in the ventral tegmental area (VTA) TH nucleus accumbens were measured after early (24 hr) late (3 weeks) withdrawal times from acute repeated cocaine treatment. Two doses (15 mg/kg i.p. or 30 i.p.) administered for 6 days to produce behavioral sensitization a challenge either 24 hr later three weeks later. At time, levels 2 saline challenge, at withdrawal, measurements made challenge. both treatment, VTA increased 125% controls...
Abstract: Expression of myelin proteins was studied in the brains 21‐day‐old normal mice and three dysmyelinating mutants—jimpy, quaking, shiverer. Total brain polyribosomes poly(A) + mRNA were translated two cell‐free systems levels synthesis basic (MBPs) proteolipid protein (PLP) determined. Synthesis MBPs quaking homozygotes at or above but PLP significantly reduced to approximately 15% control values, indicating independent effects on expression these this mutant. Immunoblot analysis...