A5053650946- Pain Mechanisms and Treatments
- Ocular Surface and Contact Lens
- Neuroscience and Neuropharmacology Research
- Neuropeptides and Animal Physiology
- Glaucoma and retinal disorders
- Neuroinflammation and Neurodegeneration Mechanisms
- Neuroscience of respiration and sleep
- Receptor Mechanisms and Signaling
- Sleep and Wakefulness Research
- Corneal surgery and disorders
- Intraocular Surgery and Lenses
- Corneal Surgery and Treatments
- Adipose Tissue and Metabolism
- Cardiovascular Disease and Adiposity
- Heart Rate Variability and Autonomic Control
- Ion Channels and Receptors
- Photoreceptor and optogenetics research
- Neurobiology and Insect Physiology Research
- Neurogenesis and neuroplasticity mechanisms
- Memory and Neural Mechanisms
- Salivary Gland Disorders and Functions
- Ion channel regulation and function
- Erythrocyte Function and Pathophysiology
- Lipid Membrane Structure and Behavior
- Family and Patient Care in Intensive Care Units
Oregon Health & Science University
2015-2024
St. James's Hospital
2020
Washington State University Vancouver
2009
Cornell University
2003-2008
The analgesic effects of morphine are mediated, in part, by periaqueductal gray (PAG) neurons that project to the rostral ventromedial medulla (RVM). Although much neural circuitry within RVM has been described, relationship between and PAG input spinal output is not known. objective this study was determine whether GABAergic from target reticulospinal neurons. Immunocytochemistry confocal microscopy revealed extensively projecting cord, two-thirds these appear be (contain GAD67...
To determine the importance of NMDA receptor (NMDAR) in pain hypersensitivity after injury, NMDAR1 (NR1) subunit was selectively deleted lumbar spinal cord adult mice by localized injection an adenoassociated virus expressing Cre recombinase into floxed NR1 mice. mRNA and dendritic protein are reduced 80% area injection, currents, but not AMPA 86–88% lamina II neurons. The spatial knock-out does alter heat or cold paw-withdrawal latencies, mechanical threshold, motor function. However,...
<h3>Abstract</h3> We previously reported that perineuronal nets (PNNs) are required for cocaine-associated memories. Perineuronal extracellular matrix primarily surrounds parvalbumin (PV)-containing, GABAergic fast-spiking interneurons (FSIs) in the medial prefrontal cortex (mPFC). Here we measured impact of acute (1 d) or repeated (5 cocaine exposure on PNNs and PV cells within prelimbic infralimbic regions mPFC. Adult rats were exposed to 1 5 d stained (using <i>Wisteria floribunda</i>...
Trigeminal afferents convey nociceptive information from the corneal surface of eye to trigeminal subnucleus caudalis (Vc). afferents, like other nociceptors, are thought use glutamate and neuropeptides as neurotransmitters. The current studies examined whether contain both vesicular transporters. Corneal Vc were identified by using cholera toxin B (CTb). project in two clusters rostral caudal borders Vc, regions that functionally distinct neurons. Thus, projecting these separately. Dual...
Repeated administration of opioids produces long-lasting changes in μ-opioid receptor (MOR) signaling that underlie behavioral such as tolerance. Mitogen-activated protein kinase (MAPK) pathways, including MAPK extracellular signal-regulated kinases (ERK1/2), are modulated by and known to produce cell signaling. Thus, we tested the hypothesis ERK1/2 activation contributes development and/or expression morphine tolerance mediated periaqueductal gray (PAG). Changes phosphorylated were assessed...
Abstract The medial prefrontal cortex (mPFC) is a major contributor to relapse cocaine in humans and reinstatement behavior rodent models of use disorder. Output from the mPFC modulated by parvalbumin (PV)-containing fast-spiking interneurons, majority which are surrounded perineuronal nets (PNNs). Here we tested whether chondroitinase ABC (ABC)- mediated removal PNNs prevented acquisition or reconsolidation self-administration memory. injections into dorsal prior training attenuated...
The medial prefrontal cortex (mPFC) is a major contributor to relapse cocaine in humans and reinstatement rodent models of use disorder. output from the mPFC potently modulated by parvalbumin (PV)-containing fast-spiking interneurons, majority which are surrounded perineuronal nets. We previously showed that treatment with chondroitinase ABC (ABC) reduced consolidation reconsolidation conditioned place preference memory. However, self-administration memories more difficult disrupt. Here we...
Some dry eye disease (DED) patients have sensitized responses to corneal stimulation, while others experience hypoalgesia. Many normal tear production, suggesting that reduced tears are not always the cause of DED sensory dysfunction. In this study, we show disruption lacrimal innervation can produce hypoalgesia without changing basal production.Injection a saporin toxin conjugate into extraorbital gland male Sprague-Dawley rats was used disrupt cholinergic gland. Tear production assessed by...
Corneal nerves mediate pain from the ocular surface, lacrimation, and blinking, all of which protect corneal surface homeostasis help preserve vision. Because pain, lacrimation blinking are rarely assessed at same time, it is not known whether these responses their underlying mechanisms have similar temporal dynamics after acute injury.We examined changes in nerve density, evoked spontaneous Sprague-Dawley male rats a superficial epithelial injury with heptanol. We also measured calcitonin...
The surface of the cornea contains highest density nociceptive nerves any tissue in body. These are responsive to a variety modalities noxious stimuli and can signal pain even when activated by low threshold stimulation. Injury corneal lead altered nerve morphology, including neuropathic changes which be associated with chronic pain. Emerging technologies that allow imaging vivo spawning questions regarding relationship between density, function. We tested whether stimulation alter...
Abstract Substance use disorder is a complex disease created in part by maladaptive learning and memory mechanisms following repeated drug use. Exposure to drug‐associated stimuli engages prefrontal cortex circuits, dysfunction of the medial (mPFC) thought underlie drug‐seeking behaviors. Growing evidence supports role for parvalbumin containing fast‐spiking interneurons (FSI) modulating cortical microcircuit activity influencing balance excitation inhibition, which can influence processes....
Abstract Sympathetic neurons that innervate the heart are located primarily in stellate ganglia (SG), which also contains project to brown adipose tissue (BAT). These studies were designed examine morphology of these two populations (cardiac‐ and BAT‐projecting) their target connectivity. We examined SG C57BL/6J mice following injections retrograde tracer cholera toxin B (CTb) conjugated Alexa Fluor 488 555, into cardiac intrascapular BAT. BAT‐projecting widely dispersed SG, while...
Trigeminal sensory afferent fibers terminating in nucleus caudalis (Vc) relay information from craniofacial regions to the brain and are known express transient receptor potential (TRP) ion channels. TRP channels activated by H(+), thermal, chemical stimuli. The present study investigated relationships among spontaneous release of glutamate, temperature, TRPV1 localization at synapses Vc. Spontaneous excitatory postsynaptic currents (sEPSCs) were recorded Vc neurons (n = 151) horizontal...