A5072467806- Neuroscience and Neural Engineering
- Hypothalamic control of reproductive hormones
- EEG and Brain-Computer Interfaces
- Circadian rhythm and melatonin
- Neural dynamics and brain function
- Neuroendocrine regulation and behavior
- Plant Reproductive Biology
- Advanced Memory and Neural Computing
- Pain Mechanisms and Treatments
- Genetic and Clinical Aspects of Sex Determination and Chromosomal Abnormalities
- Neuroscience and Neuropharmacology Research
- Ovarian function and disorders
- Photoreceptor and optogenetics research
- Stress Responses and Cortisol
- Neuropeptides and Animal Physiology
- Neurobiology and Insect Physiology Research
- Reproductive Biology and Fertility
- Plant Molecular Biology Research
- Biochemical effects in animals
- Fibromyalgia and Chronic Fatigue Syndrome Research
- Regulation of Appetite and Obesity
- Genetic Syndromes and Imprinting
- Cardiac, Anesthesia and Surgical Outcomes
- Bat Biology and Ecology Studies
- Tryptophan and brain disorders
University of California, San Diego
2017-2024
Circadian (United States)
2023
BioSurfaces (United States)
2022
Neurological Surgery
2014-2016
Oregon Health & Science University
2010-2016
Yale University
2016
Oregon State University
2009-2011
Electrophysiological devices are critical for mapping eloquent and diseased brain regions therapeutic neuromodulation in clinical settings extensively used research brain-machine interfaces. However, the existing experimental often limited either spatial resolution or cortical coverage. Here, we developed scalable manufacturing processes with a dense electrical connection scheme to achieve reconfigurable thin-film, multithousand-channel neurophysiological recording grids using platinum...
Abstract Over the past decade, stereotactically placed electrodes have become gold standard for deep brain recording and stimulation a wide variety of neurological psychiatric diseases. Current electrodes, however, are limited in their spatial resolution ability to record from small populations neurons, let alone individual neurons. Here, we report on an innovative, customizable, monolithically integrated human-grade flexible depth electrode capable up 128 channels able at 10 cm tissue. This...
Neurons that produce kisspeptin play a critical role in reproduction. However, understanding the molecular physiology of neurons has been limited by lack an vivo marker for those cells. Here, we report development Kiss1-CreGFP knockin mouse, wherein endogenous Kiss1 promoter directs expression Cre recombinase-enhanced green fluorescent protein (GFP) fusion protein. The pattern GFP brain recapitulates what described earlier male and female with prominent arcuate nucleus (ARC) (in both sexes)...
Kisspeptin (Kiss1) neurons in the rostral periventricular area of third ventricle (RP3V) provide excitatory drive to gonadotropin-releasing hormone (GnRH) control fertility. Using whole cell patch clamp recording and single-cell (sc)RT-PCR techniques targeting Kiss1-CreGFP or tyrosine hydroxylase (TH)-EGFP neurons, we characterized biophysical properties these identified critical intrinsic required for burst firing 17β-estradiol (E2)-treated, ovariectomized female mice. One-fourth RP3V Kiss1...
Abstract The Utah array powers cutting‐edge projects for restoration of neurological function, such as BrainGate, but the underlying electrode technology has itself advanced little in last three decades. Here, dual‐side lithographic microfabrication processes is exploited to demonstrate a 1024‐channel penetrating silicon microneedle (SiMNA) that scalable its recording capabilities and cortical coverage suitable clinical translation. SiMNA first with flexible backing affords compliancy brain...
In Brief Patients with functional pain disorders often complain of generalized sensory hypersensitivity, finding sounds, smells, or even everyday light aversive. The neural basis for this aversion is unknown, but it cannot be attributed to a general increase in cortical processing. Here, we quantified the threshold patients fibromyalgia, disorder thought reflect dysregulation pain-modulating systems brain. These individuals expressed discomfort at levels substantially lower than that healthy...
The ventrolateral periaqueductal gray (vlPAG) is a key structure in the descending pain modulatory circuit. Activation of circuit occurs via disinhibition GABAergic inputs onto vlPAG output neurons. In these studies, we tested hypothesis that inhibition increased during persistent inflammation, dampening activation from vlPAG. Our results indicate inflammation induced by Complete Freund's adjuvant (CFA) modulates GABA signaling differently male and female rats. CFA treatment presynaptic...
We report innovative scalable, vertical, ultra-sharp nanowire arrays that are individually addressable to enable long-term, native recordings of intracellular potentials. Stable amplitudes potentials from 3D tissue-like networks neurons and cardiomyocytes obtained. Individual electrical addressability is necessary for high-fidelity electrophysiological recordings. This study paves the way toward predictive, high-throughput, low-cost drug screening platforms.
Oestrogen-stimulated preovulatory gonadotrophin surges are temporally regulated in a way that remains not fully understood. Mammalian ovulation requires of gonadotrophin-releasing hormone (GnRH), released from specialised neurones the hypothalamus. Surge regulation is mediated by ovarian oestrogen (17β-oestradiol; E2) feedback-acting as negative signal until early afternoon pro-oestrous phase, at which point it stimulates robust increases GnRH release. Multiple lines evidence suggest role...
Trigeminal afferents convey nociceptive information from the corneal surface of eye to trigeminal subnucleus caudalis (Vc). afferents, like other nociceptors, are thought use glutamate and neuropeptides as neurotransmitters. The current studies examined whether contain both vesicular transporters. Corneal Vc were identified by using cholera toxin B (CTb). project in two clusters rostral caudal borders Vc, regions that functionally distinct neurons. Thus, projecting these separately. Dual...
In rodents, the preovulatory LH surge is temporally gated, but timing cue unknown. Estrogen primes neurons in anteroventral periventricular nucleus (AVPV) to secrete kisspeptin, which potently activates GnRH release GnRH, eliciting a of induce ovulation. Deletion circadian clock gene Bmal1 results infertility. Previous studies have found that knockout (KO) females do not display an at any time day. We sought determine whether neuroendocrine disruption contributes absence surge. Because Kiss1...
Abstract Polycystic ovary syndrome (PCOS) is a common reproductive disorder characterized by elevated androgens and antimüllerian hormone (AMH). These hormones remain throughout pregnancy, potential effects of exposure on offspring from women with PCOS largely unexplored. Expanding recent reports prenatal AMH in mice, we have fully the consequences (pAMH) lifespan first- second-generation both sexes. We also sought to elucidate mechanisms underlying pAMH-induced effects. There known...
Abstract Electrophysiological recording and stimulation are the gold standard for functional mapping during surgical therapeutic interventions as well capturing cellular activity in intact human brain. A critical component probing brain is interface material at electrode contact that electrochemically transduces signals to from free charge carriers measurement system. Here, we summarize state-of-the-art array systems context of translation use stimulating activity. We leverage parametric...
Abstract The homeodomain transcription factors sine oculis homeobox 3 (Six3) and ventral anterior 1 (Vax1) are required for brain development. Their expression in specific areas is maintained adulthood, where their functions poorly understood. To identify the roles of Six3 Vax1 neurons, we conditionally deleted each gene using Synapsin cre , a promoter targeting maturing generated syn mice. females, but not males, had reduced fertility, due to impairment luteinizing hormone (LH) surge...
Functional mapping during brain surgery is applied to define areas that control critical functions and cannot be removed. Currently, these procedures rely on verbal interactions between the neurosurgeon electrophysiologist, which can time-consuming. In addition, electrode grids are used measure activity identify boundaries of pathological versus functional regions have low resolution limited conformity surface. Here, we present development an intracranial electroencephalogram...
Abstract Hypothalamic GnRH (luteinizing hormone–releasing hormone) neurons are crucial for the hypothalamic-pituitary-gonadal (HPG) axis, which regulates mammalian fertility. Insufficient disrupts HPG axis and is often associated with genetic condition idiopathic hypogonadotropic hypogonadism (IHH). The homeodomain protein sine oculis–related homeobox 6 (Six6) required development of neurons. Although it known that Six6 specifically expressed within a more mature neuronal cell line...
The mechanisms mediating suppression of reproduction in response to decreased nutrient availability remain undefined, with studies suggesting regulation occurs within the hypothalamus, pituitary, or gonads. By manipulating glucose utilization and GLUT1 expression a pituitary gonadotrope cell model primary gonadotropes, we show GLUT1-dependent stimulation glycolysis, but not mitochondrial respiration, by reproductive neuropeptide GnRH. GnRH increases translocation extracellular membrane....
Background The suprachiasmatic nucleus (SCN) within the hypothalamus is a key brain structure required to relay light information body and synchronize cell tissue level rhythms hormone release. Specific subpopulations of SCN neurons, defined by their peptide expression, regulate output. Here we focus on vasoactive intestinal (VIP) expressing neurons SCN. VIP are known circadian reproductive function. Methods To specifically study generated novel knock out mouse line conditionally deleting...
Pathogenic SRY-box transcription factor 2 (SOX2) variants typically cause severe ocular defects within a SOX2 disorder spectrum that includes hypogonadotropic hypogonadism. We examined exome-sequencing data from large, well-phenotyped cohort of patients with idiopathic hypogonadism (IHH) for pathogenic to investigate the underlying and its associated phenotypes. identified 8 IHH individuals harboring heterozygous variable These variant proteins were tested in vitro determine whether causal...
Abstract In this study, we found that loss of the circadian clock gene Bmal1 causes disruptions throughout growth hormone (GH) axis, from hepatic expression to production urinary pheromones and pheromone-dependent behavior. First, show knockout (KO) males elicit reduced aggressive responses wild-type (WT) secrete lower levels major proteins (MUPs); however, also a liver-specific KO (liver-Bmal1-KO) produces similar reduction in MUP secretion without defect behavior, indicating decrease...