A5043532850- Advanced biosensing and bioanalysis techniques
- Monoclonal and Polyclonal Antibodies Research
- Advanced Fluorescence Microscopy Techniques
- Terahertz technology and applications
- Microbial Inactivation Methods
- Insect and Arachnid Ecology and Behavior
- Neuroscience and Neural Engineering
- Bacteriophages and microbial interactions
- Supramolecular Self-Assembly in Materials
- Neural dynamics and brain function
- Near-Field Optical Microscopy
- Luminescence and Fluorescent Materials
- RNA and protein synthesis mechanisms
- Gold and Silver Nanoparticles Synthesis and Applications
- Nanocluster Synthesis and Applications
- Hydrogen's biological and therapeutic effects
- Molecular Junctions and Nanostructures
- Biochemical and Structural Characterization
- Telomeres, Telomerase, and Senescence
- Workplace Health and Well-being
- Nanoplatforms for cancer theranostics
- Photochromic and Fluorescence Chemistry
- Inhalation and Respiratory Drug Delivery
- Polymer Surface Interaction Studies
- Biochemical effects in animals
East Carolina University
2025
Los Alamos National Laboratory
2006-2022
Center for Integrated Nanotechnologies
2009-2022
The University of Texas MD Anderson Cancer Center
2016
St Bartholomew's Hospital
1981
Worthing Hospital
1974
ConspectusMetal nanoclusters containing a few to several hundred atoms with sizes ranging from sub-nanometer ∼2 nm occupy an intermediate size regime that bridges larger plasmonic nanoparticles and smaller metal complexes. With strong quantum confinement, exhibit molecule-like properties. This Account focuses on noble are synthesized within single stranded DNA template. Compared other ligand protected nanoclusters, DNA-templated manifest intriguing physical chemical properties heavily...
We report the discovery of a DNA sequence that templates highly stable fluorescent silver nanocluster. In contrast to other templated nanoclusters have relatively short shelf-life, species in this new retains significant fluorescence for at least year. Moreover, nanocluster possesses low cellular toxicity and enhanced thermal, oxidative, chemical stability.
We report that extended exposure to broad-spectrum terahertz radiation results in specific changes cellular functions are closely related DNA-directed gene transcription. Our chip survey of expression shows whereas 89% the protein coding genes mouse stem cells do not respond applied radiation, certain activated, while other repressed. RT-PCR experiments with selected probes corresponding transcripts three groups detail effect. The response was only but also irradiation conditions dependent....
We report that terahertz (THz) irradiation of mouse mesenchymal stem cells (mMSCs) with a single-frequency (SF) 2.52 THz laser or pulsed broadband (centered at 10 THz) source results in specific heterogenic changes gene expression. The effect depends on parameters such as the duration and type degree cell differentiation. Our microarray survey RT-PCR experiments demonstrate prolonged drives mMSCs toward differentiation, while 2-hour (regardless sources) affects genes transcriptionally active...
In recent years, terahertz radiation sources are increasingly being exploited in military and civil applications. However, only a few studies have so far been conducted to examine the biological effects associated with radiation. this study, we evaluated cellular response of mesenchymal mouse stem cells exposed THz We apply low-power from both pulsed broad-band (centered at 10 THz) source CW laser (2.52 source. Modeling, empirical characterization, monitoring techniques were applied minimize...
We report innovative scalable, vertical, ultra-sharp nanowire arrays that are individually addressable to enable long-term, native recordings of intracellular potentials. Stable amplitudes potentials from 3D tissue-like networks neurons and cardiomyocytes obtained. Individual electrical addressability is necessary for high-fidelity electrophysiological recordings. This study paves the way toward predictive, high-throughput, low-cost drug screening platforms.
Biocompatible nanoparticles composed of poly(lactic-co-glycolic acid) (PLGA) are used as drug and vaccine delivery systems because their tunability in size sustained release cargo molecules. While the use toxic stabilizers such polyvinyl alcohol (PVA) limit utility PLGA, stabilizer-free PLGA rarely they can be challenging to prepare. Here, we developed a tunable, nanoparticle formulation capable encapsulating plasmid DNA demonstrated formation an elastin-like polymer hybrid with exceptional...
ArfGAP, with dual PH domain-containing protein 1/Centaurin-α(ADAP1/CentA1), is a brain-enriched and highly conserved Arf6 GTPase-activating Ras-anchoring protein. CentA1 involved in dendritic outgrowth arborization, synaptogenesis, axonal polarization by regulating the actin cytoskeleton dynamics. An increased level of its association amyloid plaques human Alzheimer's disease (AD) brain suggest role for this AD progression. To understand neurodegeneration, we crossbred KO mice J20 mouse...
Photodynamic therapy (PDT) has been explored as a therapeutic strategy to clear toxic amyloid aggregates involved in neurodegenerative disorders such Alzheimer's disease. A major limitation of PDT is off-target oxidation, which can be lethal for the surrounding cells. We have shown that novel class oligo-p-phenylene ethynylenes (OPEs) exhibit selective binding and fluorescence turn-on presence prefibrillar fibrillar disease-relevant proteins amyloid-β (Aβ) α-synuclein. Concomitant with...
In the use of non-antibody proteins as affinity reagents, diversity has generally been derived from oligonucleotide-encoded random amino acids. Although specific binders high-affinity have selected such libraries, oligonucleotides often encode stop codons and acid combinations that affect protein folding. Recently it shown antibody binding loops grafted into heterologous can confer activity to created chimeric protein. this paper, we examine elements. We first show are able graft a...
Purpose – As posited by the ecological model of health, improvements in nutrition and physical activity environments worksites may facilitate healthier dietary intakes patterns employees. This cross-sectional study describes current policies practices targeting these five large Texas-based hospitals employing approximately 40,000 adults. The paper aims to discuss issues. Design/methodology/approach environmental assessment tool survey, an observation tool, was used assess August September...
Peptides are important affinity ligands for microscopy, biosensing, and targeted delivery. However, because they can have low their targets, selection from large naïve libraries be challenging. When selecting peptidic display libraries, it is to: 1) ensure efficient display; 2) maximize the ability to select high ligands; 3) minimize effect of context on binding. The "helper cell" packaging system has been described as a tool produce filamentous phage particles based phagemid constructs with...
Protein-ligand conjugations are usually carried out in aqueous media order to mimic the environment within which conjugates will be used. In this work, we focus on conjugation of amphiphilic variants elastin-like polypeptide (ELP), short elastin (sEL), poorly water-soluble compounds like OPPVs ( p-phenylenevinylene oligomers), triarylamines, and polypyridine-metal complexes. These problematic when phase because hydrophobic ligands tend avoid exposure water, turn causes ligand self-aggregate...
Chromosome ends are shielded from exonucleolytic attack and inappropriate end-joining by terminal structures called telomeres; these potential targets for anticancer drugs. Telomeres composed of a simple DNA sequence (5?-TTAGGG-3? in humans) repeated more than thousand times, short 3? single-stranded overhang, numerous proteins. Electron microscopy has shown that the overhang pairs with complementary strand at an internal site creating small displacement loop large double-stranded “t-loop.”...
Structured water-soluble and hetero-oligomeric peptoids: the importance of peptoid-specific backbone restrictions.
We have designed a fluorescence microscope that uses unique spatial filter geometry and active feedback to follow the three dimensional motion of individual quantum dots at biologically relevant transport rates (microns/second).
We have developed a microscope system that uses active feed-back to follow individual quantum dot labeled proteins moving in three dimensions live cells at μm/s transport velocities with 100 picoseconds temporal resolution.
Intracellular access with high spatiotemporal resolution can enhance our understanding of how neurons or cardiomyocytes regulate and orchestrate network activity, this activity be affected pharmacology other interventional modalities. Nanoscale devices often employ electroporation to transiently permeate the cell membrane record intracellular potentials, which tend decrease rapidly extracellular potential amplitudes time. Here, we report innovative scalable, vertical, ultra-sharp nanowire...
Abstract Photodynamic therapy (PDT) has been explored as a therapeutic strategy to clear toxic amyloid aggregates involved in neurodegenerative disorders such Alzheimer’s disease. A major limitation of PDT is off-target oxidation, which can be lethal for the surrounding cells. We have shown that novel class oligo- p -phenylene ethynylene-based compounds (OPEs) exhibit selective binding and fluorescence turn-on presence pre-fibrillar fibrillar disease-relevant proteins amyloid-β (Aβ)...