A5078391314- Escherichia coli research studies
- Viral gastroenteritis research and epidemiology
- Salmonella and Campylobacter epidemiology
- Clostridium difficile and Clostridium perfringens research
- Enterobacteriaceae and Cronobacter Research
- Gut microbiota and health
- Viral Infections and Outbreaks Research
- Respiratory viral infections research
- SARS-CoV-2 detection and testing
- Antibiotic Resistance in Bacteria
- Advanced Drug Delivery Systems
- Bacteriophages and microbial interactions
- Influenza Virus Research Studies
- Biosensors and Analytical Detection
- Bacterial Genetics and Biotechnology
- Viral Infections and Immunology Research
- Advanced Biosensing Techniques and Applications
- SARS-CoV-2 and COVID-19 Research
- Animal Nutrition and Physiology
- Bauxite Residue and Utilization
- Nanoplatforms for cancer theranostics
- Bacterial Identification and Susceptibility Testing
- Quantum Dots Synthesis And Properties
- Regional Development and Environment
- Bacillus and Francisella bacterial research
Pacific Northwest National Laboratory
2022-2024
Los Alamos National Laboratory
2020-2022
Iowa State University
2017-2020
University of Nebraska–Lincoln
2015-2018
University of Delaware
2016
Biotechnology Institute
2016
Extraintestinal pathogenic Escherichia coli (ExPEC) strains are important pathogens that cause diverse diseases in humans and poultry. Some E. isolates from chicken feces contain ExPEC-associated virulence genes, so appear potentially pathogenic; they conceivably could be transmitted to through handling and/or consumption of contaminated meat. However, the actual extraintestinal potential chicken-source fecal is poorly understood. Here, we assessed whether healthy production chickens a model...
Bacterial infections are an urgent public health priority. The application of mRNA vaccine technology to prevent bacterial is a promising therapeutic strategy undergoing active development. This article discusses recent advances and limitations vaccines diseases provides perspectives on future research directions.
Commercial poultry farms are increasingly threatened by bacterial infections from avian pathogenic Escherichia coli (APEC) and broad-host Salmonella serovars. Recombinant attenuated vaccines (RASV) elicit cross-reactive immune responses against APEC in chickens; however, assessment of broad protection is lacking. Probiotics boost chicken immunity improve vaccination responses. The objective this study was to determine whether the RASV, probiotics, or their combination had Salmonella. White...
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Cattle hides are a main source of enterohemorrhagic Escherichia coli (EHEC) contamination beef carcasses. The objectives this study were to (1) determine the prevalence "top 6" non-O157 plus O157:H7 EHEC (EHEC-7) on feedlot cattle and their matched preintervention carcasses; (2) assess agreement among detection methods for these matrices; (3) conduct molecular risk assessment EHEC-7 isolates. Samples from 576 obtained at commercial harvest facility tested by culture-based method polymerase...
With the majority of conventional cage (CC) laying facilities transitioning into cage-free (CF) systems in near future, it is important to characterize biological markers health layers housed commercial housings for sustainable production. The objectives this study were compare i) blood markers, that heterophil:lymphocyte (H:L) ratios and susceptibility avian pathogenic Escherichia coli (APEC) ii) lung ceca microbiome between hens at different maturity stages CC CF farms. Laying 3 randomly...
Biocompatible nanoparticles composed of poly(lactic-co-glycolic acid) (PLGA) are used as drug and vaccine delivery systems because their tunability in size sustained release cargo molecules. While the use toxic stabilizers such polyvinyl alcohol (PVA) limit utility PLGA, stabilizer-free PLGA rarely they can be challenging to prepare. Here, we developed a tunable, nanoparticle formulation capable encapsulating plasmid DNA demonstrated formation an elastin-like polymer hybrid with exceptional...
Early and accurate diagnosis of respiratory pathogens associated outbreaks can allow for the control spread, epidemiological modeling, targeted treatment, decision making–as is evident with current COVID-19 pandemic. Many infections share common symptoms, making them difficult to diagnose using only syndromic presentation. Yet, delays in getting reference laboratory tests limited availability poor sensitivity point-of-care tests, most-relied upon method clinical practice today. Here, we...
Since 1982, specific serotypes of Shiga toxin-producing Escherichia coli (STEC) have been recognized as significant foodborne pathogens acquired from contaminated beef and, more recently, other food products. Cattle are the major reservoir hosts these organisms, and while there advancements in safety practices industry standards, STEC still remains prevalent within cattle operations with hides implicated sources carcass contamination. To investigate whether composition hide-specific...
Chickens are a major source of protein worldwide, yet infectious diseases continue to threaten the poultry industry. Avian pathogenic Escherichia coli (APEC), subgroup extraintestinal E. (ExPEC), causes colibacillosis in chickens resulting economic loss because treatment, condemnation products, and death. In this study, we evaluated recombinant antigens (rAg) vaccine combining common ExPEC surface proteins EtsC, OmpA, OmpT, TraT for broad protective potential against APEC infections...
Antibiotic resistance is a threat to public health. Many clinically relevant antibiotic genes are carried on plasmids that can be transferred other bacterial members in the gastrointestinal tract. The current study used murine model transfer of large plasmid from foodborne Salmonella strain gut commensal E. coli We found different mouse genetic backgrounds and diversity microbial communities influenced level Escherichia acquired This suggests complexity community host genetics influence...
Avian pathogenic Escherichia coli (APEC) causes extraintestinal infections in poultry. Vaccines targeting APEC chickens have been partially successful, but many lack heterologous protection. Recombinant attenuated Salmonella vaccine (RASV) strains can induce broad immunity against and be modified to deliver E. antigens. Along with characteristics, understanding the host response is crucial for developing improved vaccines. The objectives of this study were evaluate responses vaccination an...
Near-infrared (NIR) emitting quantum dots (QDs) with emission in the biological transparency windows (NIR-I: 650-950 nm and NIR-II: 1000-1350 nm) are promising candidates for deep-tissue bioimaging. However, they typically contain toxic heavy metals such as cadmium, mercury, arsenic, or lead. We report on biocompatibility of high brightness CuInSexS2-x/ZnS (CISeS/ZnS) QDs a tunable covering visible to NIR (550-1300 peak emission) quantify transmission their photoluminescence through multiple...
Antibiotic-resistant bacterial infections are difficult to treat, producing a burden on healthcare and the economy. Extraintestinal pathogenic Escherichia coli (ExPEC) strains frequently carry antibiotic resistance genes, cause outside of intestine, causative agents hospital-acquired infections. Developing prevention strategy against this pathogen is challenging due its antigenic diversity. E. common pilus (ECP) found in ExPEC may serve as antigen induce protection several serotypes. In...
Enterohemorrhagic Escherichia coli (EHEC) strains are foodborne pathogens carried in the intestinal tracts of ruminants and shed feces. High concentrations (≥104 colony-forming units [CFU]/g) EHEC cattle feces associated with contamination hides, subsequently, carcasses beef. Several studies using agar media have quantified O157 but few non-O157 samples from cattle. Thus, objective this study was to determine concentration cattle, characterize isolates for their virulence potential. Two...
Summary Cattle hides are an important source of enterohaemorrhagic Escherichia coli (EHEC) carcass contamination at slaughter. Seven EHEC serogroups adulterants in raw, non‐intact beef: O26, O45, O103, O111, O121, O145 and O157. The objective this study was to estimate the probability for hide with among US market beef cows slaughter test effects season geographic region on prevalence contamination. Hides ( n = 800) were swabbed immediately after exsanguination, prior removal. Cows sampled...
Foodborne pathogens are a public health threat globally. Shiga toxin-producing Escherichia coli (STEC), particularly O26, O111, and O157 STEC, often associated with foodborne illness in humans. To create effective preharvest interventions, it is critical to understand which factors STEC strains use colonize the gastrointestinal tract of cattle, serves as reservoir for these pathogens. Several colonization known, but little understood about initial factors. Our objective was identify via...
Detection methods that do not require nucleic acid amplification are advantageous for viral diagnostics due to their rapid results. These platforms could provide information both accurate diagnoses and pandemic surveillance. Influenza virus is prone pandemic-inducing genetic mutations, so there a need apply these detection influenza diagnostics. Here, we analyzed the Fast Evaluation of Viral Emerging Risks (FEVER) pipeline on ultrasensitive platforms, including waveguide-based optical...