A5088091907- Virus-based gene therapy research
- Cancer Research and Treatments
- Viral Infectious Diseases and Gene Expression in Insects
- interferon and immune responses
- Transgenic Plants and Applications
- Herpesvirus Infections and Treatments
- Animal Virus Infections Studies
- Wound Healing and Treatments
- RNA Interference and Gene Delivery
- Silk-based biomaterials and applications
- Laser Applications in Dentistry and Medicine
- Viral gastroenteritis research and epidemiology
- Cholinesterase and Neurodegenerative Diseases
- Cytomegalovirus and herpesvirus research
- CAR-T cell therapy research
- Cancer, Hypoxia, and Metabolism
- Antimicrobial Peptides and Activities
- Electrospun Nanofibers in Biomedical Applications
- Pluripotent Stem Cells Research
- Surgical Sutures and Adhesives
- Plant Virus Research Studies
- Vibrio bacteria research studies
- Plant tissue culture and regeneration
- Liver physiology and pathology
- Monoclonal and Polyclonal Antibodies Research
Arizona State University
2015-2024
Tempe Union High School District
2013-2021
National Center for Infectious Diseases
2013
Hebrew University of Jerusalem
2008
University of Arizona
2008
United States Army
2008
Extraintestinal pathogenic Escherichia coli (ExPEC) strains are important pathogens that cause diverse diseases in humans and poultry. Some E. isolates from chicken feces contain ExPEC-associated virulence genes, so appear potentially pathogenic; they conceivably could be transmitted to through handling and/or consumption of contaminated meat. However, the actual extraintestinal potential chicken-source fecal is poorly understood. Here, we assessed whether healthy production chickens a model...
Pluripotent stem cell (PSC)-derived organoids have emerged as novel multicellular models of human tissue development but display immature phenotypes, aberrant fates, and a limited subset cells. Here, we demonstrate that integrated analysis engineering gene regulatory networks (GRNs) in PSC-derived multilineage liver direct maturation vascular morphogenesis vitro. Overexpression PROX1 ATF5, combined with targeted CRISPR-based transcriptional activation endogenous CYP3A4, reprograms GRNs...
The concept of using cholinesterase bioscavengers for prophylaxis against organophosphorous nerve agents and pesticides has progressed from the bench to clinical trial. However, supply native human proteins is either limited (e.g., plasma-derived butyrylcholinesterase erythrocytic acetylcholinesterase) or nonexisting (synaptic acetylcholinesterase). Here we identify a unique form recombinant that mimics enzyme assembly into tetramers; this provides extended effective pharmacokinetics...
Ebola hemorrhagic fever is an acute and often deadly disease caused by virus (EBOV). The possible intentional use of this against human populations has led to design vaccines that could be incorporated into a national stockpile for biological threat reduction. We have evaluated the immunogenicity efficacy EBOV vaccine candidate in which viral surface glycoprotein biomanufactured as fusion monoclonal antibody recognizes epitope glycoprotein, resulting production immune complexes (EICs)....
A distinct pathovar of Salmonella enterica serovar Typhimurium, ST313, has emerged in sub-Saharan Africa as a major cause fatal bacteremia young children and HIV-infected adults. D23580, multidrug resistant clinical isolate was previously shown to have undergone genome reduction manner that resembles the more human-restricted pathogen, Typhi. It since been through tissue distribution studies D23580 is able establish an invasive infection chickens. However, it remains unclear whether ST313...
Solid cancers that metastasize to the lungs represent a major therapeutic challenge. Current treatment paradigms for lung metastases consist of radiation therapy, chemotherapies, and surgical resection, but there is no single or combination effective all tumor types. To address this, oncolytic myxoma virus (MYXV) engineered express human necrosis factor (vMyx-hTNF) was tested after systemic administration in an immunocompetent mouse K7M2-Luc metastatic osteosarcoma model. Virus therapy...
Tissue approximation and repair have been conventionally performed with sutures staples, but these means are inherently traumatic. using laser-responsive nanomaterials can lead to rapid tissue sealing repair, is an attractive alternative existing clinical methods. Here, we demonstrate the use of laser-activated nanosealants (LANS) gold nanorods (GNRs) embedded in silk fibroin polypeptide matrices. The adaptability LANS for soft tissues demonstrated two different modalities: insoluble thin...
Virus-like particles (VLPs) are an active area of vaccine research, development and commercialization. Mucosal administration VLPs provides attractive avenue for delivery vaccines with the potential to produce robust immune responses. Nasal oral routes particularly intriguing due differential activation mucosa-associated lymphoid tissues. We compared both intranasal a panel toll-like receptor (TLR) agonists (TLR3, 5, 7, 7/8, 9) determine mucosal adjuvant activity these immunomodulators....
The low pH of the stomach serves as a barrier to ingested microbes and must be overcome or bypassed when delivering live bacteria for vaccine probiotic applications. Typically, impact acidity on bacterial survival is evaluated in vitro, there are no small animal models evaluate these effects vivo. To better understand effect this attenuated Salmonella vaccines, which often very sensitive pH, we investigated value histamine mouse model application. A gastric compartment was transiently...
Numerous treatments have been developed to promote wound healing based on current understandings of the process. Hemorrhaging, clotting, and associated inflammation regulate early healing. We investigated treatment with a virus-derived immune modulating serine protease inhibitor (SERPIN), Serp-1, which inhibits thrombolytic proteases inflammation, in mouse excisional model. Saline or recombinant Serp-1 were applied directly wounds as single doses 1 μg 2 µg two boluses. A chitosan-collagen...
Immunopathogenesis in systemic viral infections can induce a septic state with leaky capillary syndrome, disseminated coagulopathy, and high mortality limited treatment options. Murine gammaherpesvirus-68 (MHV-68) intraperitoneal infection is gammaherpesvirus model for producing severe vasculitis, colitis lethal hemorrhagic pneumonia interferon gamma receptor-deficient (IFNγR-/-) mice. In prior work, myxomavirus-derived Serp-1 or derivative peptide S-7 (G305TTASSDTAITLIPR319) induced immune...
Therapeutically valuable proteins are often rare and/or unstable in their natural context, calling for production solutions heterologous systems. A relevant example is that of the stress-induced, normally rare, and naturally "read-through" human acetylcholinesterase variant, AChE-R. AChE-R shares its active site with synaptic AChE-S which target poisonous organophosphate anticholinesterase insecticides such as parathion metabolite paraoxon. Inherent overproduction under intoxication confers...
Discoveries associated with antibacterial activity of hydrated clays necessitate assessments in vivo efficacy, practical use and safety. Surface properties can lead to variations the composition abundance bound compounds or ions, thus affecting activity. Since exchangeable metal ions released from clay surface are responsible for vitro activity, we evaluated efficacy four natural (one illite clay, two montmorillonite one kaolinite clay) three ion-exchanged, against superficial, cutaneous...
It is widely anticipated that a prophylactic vaccine may be needed to control the HIV/AIDS epidemic worldwide. Despite over two decades of research, against HIV-1 remains elusive, although recent clinical trial has shown promising results. Recent studies have focused on highly conserved domains within such as membrane proximal external region (MPER) envelope glycoprotein, gp41. MPER been play critical roles in mucosal transmission HIV-1, though this peptide poorly immunogenic its own. Here...