A5044068024- CRISPR and Genetic Engineering
- Pluripotent Stem Cells Research
- Liver physiology and pathology
- Renal and related cancers
- Viral Infections and Immunology Research
- Tissue Engineering and Regenerative Medicine
- Advanced biosensing and bioanalysis techniques
- RNA Interference and Gene Delivery
- Innovation and Socioeconomic Development
- dental development and anomalies
- CAR-T cell therapy research
- Dental Implant Techniques and Outcomes
- Legume Nitrogen Fixing Symbiosis
- Dental Trauma and Treatments
- 3D Printing in Biomedical Research
- bioluminescence and chemiluminescence research
- Bone Tissue Engineering Materials
- Immunotherapy and Immune Responses
- Plant Pathogenic Bacteria Studies
- Genomics and Phylogenetic Studies
- Dental materials and restorations
- Dental Radiography and Imaging
- Neurobiology and Insect Physiology Research
- Photoreceptor and optogenetics research
- Plant Physiology and Cultivation Studies
University of Pittsburgh
2020
Arizona State University
2017-2020
Oklahoma State University
2016
The CRISPR-Cas9 system has raised hopes for developing personalized gene therapies complex diseases. Its application genetic and epigenetic in humans raises concerns over immunogenicity of the bacterially derived Cas9 protein. Here we detect antibodies to Streptococcus pyogenes (SpCas9) at least 5% 143 healthy individuals. We also report pre-existing human CD8+T cell immunity majority individuals screened. identify two immunodominant SpCas9 T epitopes HLA-A*02:01 using an enhanced prediction...
Pluripotent stem cell (PSC)-derived organoids have emerged as novel multicellular models of human tissue development but display immature phenotypes, aberrant fates, and a limited subset cells. Here, we demonstrate that integrated analysis engineering gene regulatory networks (GRNs) in PSC-derived multilineage liver direct maturation vascular morphogenesis vitro. Overexpression PROX1 ATF5, combined with targeted CRISPR-based transcriptional activation endogenous CYP3A4, reprograms GRNs...
Abstract The application of Cas9 for genetic and epigenetic therapies in humans raises concerns over immunogenicity this foreign protein. We report pre-existing human CD8+ T cell immunity to Streptococcus pyogenes the majority healthy individuals screened. In a proof-of-principle study, we demonstrate that protein can be modified eliminate immunodominant epitopes through targeted mutation while preserving its function specificity.
The genus Pantoea is a predominant member of host-associated microbiome. We here report on the genomic analysis eucrina strain Russ that was isolated from trashcan at Oklahoma State University, Stillwater, OK. draft genome consists 3,939,877 bp DNA with 3704 protein-coding genes and 134 RNA genes. This first sequence eucrina. Genomic revealed metabolic versatility involved in metabolism transport all amino acids as well glucose, fructose, mannose, xylose, arabinose galactose, suggesting...
SUMMARY Pluripotent stem cell (PSC)-derived organoids are emerging as novel human-based microphysiological models but display immature phenotypes with limited subsets of endothelial or stromal cells. Here we demonstrate that in vitro manipulation gene regulatory networks (GRNs) PSC-derived liver selected either through computational analysis targeted tissue design can advance maturation . Through an unbiased comparison the genetic signature mature livers, identify downregulated GRNs fetal...