A5048039026- Cholinesterase and Neurodegenerative Diseases
- Transgenic Plants and Applications
- Insect and Pesticide Research
- Insect Resistance and Genetics
- Venous Thromboembolism Diagnosis and Management
- Pesticide Exposure and Toxicity
- Acute Ischemic Stroke Management
- Plant tissue culture and regeneration
- Cardiac tumors and thrombi
Wake Forest University
2023
Arizona State University
2008-2010
The concept of using cholinesterase bioscavengers for prophylaxis against organophosphorous nerve agents and pesticides has progressed from the bench to clinical trial. However, supply native human proteins is either limited (e.g., plasma-derived butyrylcholinesterase erythrocytic acetylcholinesterase) or nonexisting (synaptic acetylcholinesterase). Here we identify a unique form recombinant that mimics enzyme assembly into tetramers; this provides extended effective pharmacokinetics...
Organophosphorous pesticides and nerve agents inhibit the enzyme acetylcholinesterase at neuronal synapses in neuromuscular junctions. The resulting accumulation of acetylcholine overwhelms regulatory mechanisms, potentially leading to seizures death from respiratory collapse. While current therapies are only capable reducing mortality, elevation serum levels related butyrylcholinesterase (BChE) by application purified protein as a bioscavenger organophosphorous compounds is effective...
Abstract Background Human cholinesterases can be used as a bioscavenger of organophosphate toxins pesticides and chemical warfare nerve agents. The practicality this approach depends on the availability human enzymes, but because inherent supply regulatory constraints, suitable production system is yet to identified. Results As promising alternative, we report creation "hairy root" organ cultures derived via Agrobacterium rhizogenes -mediated transformation from...