Solid cancers that metastasize to the lungs represent a major therapeutic challenge. Current treatment paradigms for lung metastases consist of radiation therapy, chemotherapies, and surgical resection, but there is no single or combination effective all tumor types. To address this, oncolytic myxoma virus (MYXV) engineered express human necrosis factor (vMyx-hTNF) was tested after systemic administration in an immunocompetent mouse K7M2-Luc metastatic osteosarcoma model. Virus therapy...

Oncolytic viruses can target neoplasms, triggering oncolytic and immune effects. Their delivery to melanoma lesions remains challenging. Bone-marrow-derived mesenchymal stem cells (MSCs) were shown be permissive for myxoma virus (MYXV), allowing its transfer cells, leading their killing. Involvement of progeny was demonstrated in the from MSCs co-cultured cells. The inhibitory effect on foci formation murine lungs revealed using previously with MYXV-infected MSCs. Virus accumulation...

Antibody detection and accurate diagnosis of tropical diseases is essential to help prevent the spread disease. However, most methods lack cost-effectiveness field portability, which are features for achieving in a timely manner. To address this, 3D-printed oblate spheroid sample chambers were fabricated measure green light scattering gold nanoparticles using an optical caustic focus detect antibodies. Scattering signals 20-200 nm laser compared emitting diode (LED) source Mie theory. The...

Although there are extensive literature reports on the use of gold nanoparticle (AuNP) based homogeneous assays for detection biomolecules, very few experimental description and procedures involving their preparation described. In this study, AuNPs conjugated to Bovine Serum Albumin or Envelope protein from Dengue II were developed as a immunoassay antibody detection. We report here optimization key parameters prepare an like conjugation concentration, centrifugation time, electrolyte...

Abstract Antibody detection and accurate diagnosis of tropical diseases is essential to help prevent the spread disease. However, most methods lack cost-effectiveness field-portability, which are features for achieving in a timely manner developing countries. To address this problem, transparent 3D printed sample chambers with total volume 700 microliters an oblate spheroid shape were fabricated measure green light scattering gold nanoparticles using optical caustic focus detect antibodies....

Abstract Oncolytic viruses (OV) selectively replicate in and lyse tumor cells provide stimulation to the immune system, representing a promising therapeutic option development treat cancers that do not respond well treatment with checkpoint inhibitors. Myxoma virus (MYXV) is member of Pox family double stranded DNA infects cancer cell lines humans other species. The genome MYXV relatively large amenable engineering for expression transgenic proteins, making it an excellent oncolytic...

<h3>Background</h3> Despite improvements with new therapeutics, multiple myeloma (MM) patients still relapse and become refractory. Myxoma virus (MYXV) selectively replicates in human tumor cells stimulates the immune system. MYXV kills patient MM spares normal progenitors. also eradicates growth of a disseminated mouse vivo. is large, double stranded DNA poxvirus, has genome size amenable to insertion transgenes. We generated carrying IL-12 decorin. an modulator that activates T- NK-cells....

Abstract Oncolytic Viruses (OV) selectively replicate in and lyse tumor cells provide stimulation to the immune system. This represents a promising therapeutic option for cancer patients that do not respond well treatment with checkpoint inhibitors. Myxoma virus (MYXV) is member of Poxviridae family double stranded DNA viruses. The natural host MYXV subset lagomorphs, but can infect cell lines humans other species. genome relatively large amenable engineering expression transgenes making it...

Abstract Viral infections often result in quasi-species of virus strains that can have dramatic impacts on disease outcomes or experimental results. However, sequencing viruses to determine strain composition is time consuming and cost-prohibitive. Rapid, cost-effective methods are needed for accurate measurement diversity order design appropriate treatment, be useful many systems. We developed a novel molecular method simple detection RNA genetic variants using Tentacle probes coupled with...

Virus infections often result in quasispecies of viral strains that can have dramatic impacts on disease outcomes. However, sequencing viruses to determine strain composition is time consuming and cost-prohibitive. Rapid, cost-effective methods are needed for accurate measurement virus diversity understand evolution be useful experimental systems. We developed a novel molecular method sequence-specific detection RNA genetic variants called Tentacle Probes. The probes modified beacons...

Sensitivity and specificity. Thirty samples of viral cDNA were subject to test if they target or non-target depending on the Tentacle Probe used compared a negative positive control. A) Test for Clone-13 Probe, B) Armstrong Probe. Yellow lines represent false positive, purple negative, red line is average control, black True are in blue true negatives gray. (TIFF 4096 kb)

Oncolytic virotherapy is an innovative alternative to more conventional cancer therapies that based on the ability of oncolytic viruses specifically target and kill tumor cells. This therapy can activate innate adaptive immune response body achieving destruction cells by system. Myxoma virus a poxvirus infects in European rabbits, also replicate variety human lines, which makes it ideal candidate for virotherapy. However, dynamic mechanisms between are not completely understood. Mathematical...

<h3>Background</h3> Oncolytic Viruses (OV) selectively replicate in and lyse tumor cells provide stimulation to the immune system. This represents a promising therapeutic option for cancer patients that do not respond well treatment with checkpoint inhibitors. Myxoma virus (MYXV) is member of Pox family double stranded DNA viruses. The natural host MYXV subset rabbits hares, but able infect cell lines humans other species. genome relatively large amenable engineering expression transgenic...

<h3>Background</h3> Oncolytic Viruses (OV) selectively replicate in and lyse tumor cells provide stimulation to the immune system. This represents a promising therapeutic option for cancer patients that do not respond well treatment with checkpoint inhibitors. Myxoma virus (MYXV) is member of Poxv family double stranded DNA viruses. The natural host MYXV subset rabbits hares, but can infect cell lines humans other species. genome relatively large amenable engineering expression transgenes...

Abstract Oncolytic Viruses (OV) selectively replicate in and lyse tumor cells provide stimulation to the immune system. This represents a promising therapeutic option for cancer patients that do not respond well treatment with checkpoint inhibitors. Myxoma virus (MYXV) is member of Poxviridae family double stranded DNA viruses. The natural host MYXV subset lagomorphs, but able infect cell lines humans other species. genome relatively large amenable engineering expression transgenic proteins...

Abstract Oncolytic viruses (OV) selectively replicate in and lyse tumor cells provide stimulation to the immune system, representing a promising therapeutic option development treat cancers that do not respond well treatment with checkpoint inhibitors. Myxoma virus (MYXV) is member of Pox family double stranded DNA viruses. The natural host MYXV subset rabbits hares, but can infect cancer cell lines humans other species. genome relatively large amenable engineering for expression transgenes...

Abstract A quantitative systems pharmacology (QSP) model of oncolytic immunotherapies based on the myxoma virus (MYXV) platform was constructed to project systemic cytokine exposure after intravenous (IV) administration, and determine safe doses. Oncolytic viruses selectively replicate in lyse tumor cells provide stimulation immune system. The genome MYXV is relatively large amenable engineering for expression transgenic proteins. QSP mechanistically describes infection competent cells,...

Abstract Oncolytic viruses (OV) selectively replicate in and lyse tumor cells provide stimulation to the immune system, representing a promising therapeutic option development treat cancers that do not respond well treatment with checkpoint inhibitors. Myxoma virus (MYXV) is member of Pox family double stranded DNA viruses. The natural host MYXV subset rabbits hares, but able infect cancer cell lines humans other species. genome relatively large amenable engineering for expression transgenic...

<h3>Background</h3> Myxoma virus (MYXV) has been shown to selectively infect cancer cells in humans vitro and inhibit tumor growth mice. The genome of MYXV is large amenable engineering for expression multiple transgenes. We armed with mouse or human IL-12 decorin. an immune modulator. Cellular responses decorin include cell intrinsic signaling effects, matrix remodeling, inhibition the TGF-beta pathway. hypothesized that would be effective anti-tumor therapy. current work describes...