A5087743116- Advanced Biosensing Techniques and Applications
- Biosensors and Analytical Detection
- Advanced biosensing and bioanalysis techniques
- Lipid Membrane Structure and Behavior
- Tuberculosis Research and Epidemiology
- Mycobacterium research and diagnosis
- Influenza Virus Research Studies
- Immune Response and Inflammation
- Salmonella and Campylobacter epidemiology
- Monoclonal and Polyclonal Antibodies Research
- Metabolomics and Mass Spectrometry Studies
- Bacteriophages and microbial interactions
- Pneumonia and Respiratory Infections
- Genetics, Bioinformatics, and Biomedical Research
- Mosquito-borne diseases and control
- Vibrio bacteria research studies
- Escherichia coli research studies
- SARS-CoV-2 and COVID-19 Research
- Viral gastroenteritis research and epidemiology
- SARS-CoV-2 detection and testing
- Viral Infections and Outbreaks Research
- Respiratory viral infections research
- Bacterial Identification and Susceptibility Testing
- Viral Infections and Vectors
- Genomics and Phylogenetic Studies
Lawrence Berkeley National Laboratory
2024-2025
Los Alamos National Laboratory
2015-2024
Amrita Vishwa Vidyapeetham
2024
Los Alamos National Security (United States)
2019
University of New Mexico
2001-2018
New Mexico Consortium
2014-2018
City Of Hope National Medical Center
2009
Beckman Research Institute
2009
Center for Integrated Nanotechnologies
2009
Agave BioSystems (United States)
2005
This "Applications" article focuses on superquenching of the fluorescence conjugated polyelectrolytes and related fluorescent its applications to biosensing. Superquenching can occur for both aqueous solutions these polymers as well various supported formats. For biosensing formats are generally most practical. Three practical sensing reviewed: nucleic acids, protease enzyme activity assays kinase/phosphatase based metal-ion-mediated superquenching.
Optical phenomena such as fluorescence, phosphorescence, polarization, interference and non-linearity have been extensively used for biosensing applications. waveguides (both planar fiber-optic) are comprised of a material with high permittivity/high refractive index surrounded on all sides by materials lower indices, substrate the media to be sensed. This arrangement allows coupled light propagate through waveguide total internal reflection generates an electromagnetic wave-the evanescent...
Background Current diagnostic methods for tuberculosis (TB), a major global health challenge that kills nearly two million people annually, are time-consuming and inadequate. During infection number of bacterial molecules play role in the infective process released have been proposed as biomarkers early TB diagnosis. Antigen 85 (Ag85) is most abundant secreted protein, potential target this approach. One bottlenecks direct detection such targets availability robust, sensitive, specific...
Multiplex biomarker quantitation is ideal for tracking the progression of a disease, but sensitive, specific, and quantitative multiplex biosensing remains challenging. We achieved total internal reflection with two lasers in single planar optical waveguide by coupling 532 nm laser light into diffraction grating 635 directly thin film same waveguide. confirmed that evanescent fields generated can excite fluorescent dyes (Alexa Fluor Alexa 647) held to surface through biotin-streptavidin...
Microbiology plays an important role in most sectors. Future progress critical areas requires diverse workforce development. We outline a pathway program that aims to provide equitable exposure high-impact research experiences and course-based instruction crucial training growing of microbiology (phage discovery, synthetic biology data science/AI).
We report the modular synthesis of robust, biotinylated biantennary sialylglycoconjugates and their ability to differentiate between two type A influenza strains. This is first demonstration glycoconjugate-based discriminatory capture detection strains intact virus, in presence innate enzymatic activity viral neuraminidases. also demonstrate a "carboassay" using glycoconjugates as reporter elements, which therefore, does not require antibodies. The viruses potential benefit for clinical diagnostics.
Abstract The many respiratory viruses that cause influenza-like illness (ILI) are reported and tracked as one entity, defined by the CDC a group of symptoms include fever 100 degrees Fahrenheit cough and/or sore throat. In United States alone, ILI impacts 9-49 million people every year. While tracking single clinical syndrome is informative in respects, underlying differ their parameters outbreak properties. Most existing models treat either virus or whole. However, there need for capable...
Abstract The separation of biomarkers from blood is straightforward in most molecular biology laboratories. However, resource-limited settings, allowing for the successful removal diagnostic applications, not always possible. situation further complicated by need to separate hydrophobic signatures such as lipids blood. Herein, we present a microfluidic device capable centrifugal serum at point with system that compatible are both hydrophilic and hydrophobic. cross-flow filtration separates...
Early and accurate diagnosis of respiratory pathogens associated outbreaks can allow for the control spread, epidemiological modeling, targeted treatment, decision making–as is evident with current COVID-19 pandemic. Many infections share common symptoms, making them difficult to diagnose using only syndromic presentation. Yet, delays in getting reference laboratory tests limited availability poor sensitivity point-of-care tests, most-relied upon method clinical practice today. Here, we...
No single biomarker can accurately predict disease. An ideal biodetection technology should be capable of the quantitative, reproducible, and sensitive detection a limited suite such molecules. To this end, we have developed multiplex assay for protective antigen lethal factor Bacillus anthracis toxin using semiconductor quantum dots as fluorescence reporters on our waveguide-based biosensor platform. The platform is extendable to wide array biomarkers, facilitating rapid, sensitive,...
Rapid, on-site diagnostics allow for timely intervention and response warfighter support, environmental monitoring, global health needs. Portable optical biosensors are being widely pursued as a means of achieving fieldable biosensing due to the potential speed accuracy detection. We recently developed portable engineered analytic sensor with automated sampling (PEGASUS) goal developing fieldable, generalizable platform. Here, we detail development PEGASUS’s sensing hardware use test-bed...
<title>Abstract</title> The complexity and variability of biological data has promoted the increased use machine learning methods to understand processes predict outcomes. These same features complicate reliable, reproducible, interpretable, responsible such methods, resulting in questionable relevance derived Here we systematically explore challenges associated with applying using a well characterized vitro experimental system. We evaluated factors that vary while classifers: 1) type...
The sensor team at the Los Alamos National Laboratory has developed a waveguide-based optical biosensor for detection of biomarkers associated with disease. We have previously demonstrated application this technology to sensitive carcinoembryonic antigen in serum and nipple aspirate fluid from breast cancer patients. In publication, we report improvements that will facilitate transition point-of-care diagnostic system and/or robust research tool. first improvement involved replacing...
Exposure to chronic hypoxia induces erythropoietin (EPO) production facilitate oxygen delivery hypoxic tissues. Previous studies from our laboratory found that ovariectomy (OVX) exacerbates the polycythemic response and treatment with 17β-estradiol (E2-β) inhibits this effect. We hypothesized E2-β decreases EPO gene expression during hypoxia. Because can induce nitric oxide (NO) NO attenuate synthesis, we further inhibition of is mediated by NO. These hypotheses were tested in OVX...
Abstract Bacteremia is a leading cause of death in sub-Saharan Africa where childhood mortality rates are the highest world. The early diagnosis bacteremia and initiation treatment saves lives, especially high-disease burden areas. However, diagnosing challenging for clinicians, children presenting with co-infections such as malaria HIV. There an urgent need rapid method detecting pediatric patients co-morbidities to inform treatment. In this manuscript, we have developed clinically...