A5086209074- MicroRNA in disease regulation
- RNA modifications and cancer
- Cancer-related molecular mechanisms research
- RNA Interference and Gene Delivery
- Immunotherapy and Immune Responses
- Cancer Cells and Metastasis
- Circular RNAs in diseases
- Immune Cell Function and Interaction
- Cancer Immunotherapy and Biomarkers
- Epigenetics and DNA Methylation
- Pancreatic and Hepatic Oncology Research
- Histone Deacetylase Inhibitors Research
- Breast Cancer Treatment Studies
- Extracellular vesicles in disease
- Nanoplatforms for cancer theranostics
- CAR-T cell therapy research
- RNA Research and Splicing
- Phagocytosis and Immune Regulation
- interferon and immune responses
- Immune cells in cancer
- Cancer Genomics and Diagnostics
- Pancreatic function and diabetes
- Neuroendocrine regulation and behavior
- Endometriosis Research and Treatment
- Genetics, Aging, and Longevity in Model Organisms
Michigan State University
2019-2025
Laboratoire de Biochimie
2009-2023
Dartmouth College
2004-2019
Multidisciplinary Digital Publishing Institute (Switzerland)
2019
Victor (Japan)
2019
Istituto Nazionale Tumori IRCCS "Fondazione G. Pascale"
2019
Hospital General Universitario de Alicante Doctor Balmis
2018
Van Andel Institute
2014-2018
Dartmouth–Hitchcock Medical Center
2008-2014
King's College London
2014
Abstract MicroRNAs (miRNAs) are a new class of short noncoding regulatory RNAs (18–25 nucleotides) that involved in diverse developmental and pathologic processes. Altered miRNA expression has been associated with several types human cancer. However, most studies did not establish whether changes occurred within cells undergoing malignant transformation. To obtain insight into deregulation breast cancer, we implemented an situ hybridization (ISH) method to reveal the spatial distribution...
Abstract V-domain Ig suppressor of T cell activation (VISTA) is a potent negative regulator T-cell function that expressed on hematopoietic cells. VISTA levels are heightened within the tumor microenvironment, in which its blockade can enhance antitumor immune responses mice. In humans, related programmed death 1 (PD-1) pathway has shown great potential clinical immunotherapy trials. Here, we report structure human and examine lymphocyte regulation cancer. predominantly compartment with...
The causal basis of vertebrate complexity has been sought in genome duplication events (GDEs) that occurred during the emergence vertebrates, but evidence beyond coincidence is wanting. MicroRNAs (miRNAs) have recently identified as a viable factor increasing organismal through action these approximately 22-nt noncoding RNAs regulating gene expression. Because miRNAs are continuously being added to animalian genomes, and, once integrated into regulatory network, strongly conserved primary...
MicroRNAs (miRNAs) regulate gene expression. It has been suggested that obtaining miRNA expression profiles can improve classification, diagnostic, and prognostic information in oncology. Here, we sought to comprehensively identify the miRNAs are overexpressed lung cancer by conducting microarray profiling on normal versus adjacent cancers from transgenic mice. We found miR-136, miR-376a, miR-31 were each prominently murine cancers. Real-time RT-PCR situ hybridization (ISH) assays confirmed...
Abstract In the past few years, field of cancer immunotherapy has made great progress and is finally starting to change way treated. We are now learning that multiple negative checkpoint regulators (NCR) restrict ability T-cell responses effectively attack tumors. Releasing these brakes through antibody blockade, first with anti-CTLA4 followed by anti-PD1 anti-PDL1, emerged as an exciting strategy for treatment. More recently, a new NCR surfaced called V-domain immunoglobulin (Ig)-containing...
Abstract How complex body plans evolved in animals such as fruit flies and vertebrates, compared to the relatively simple jellyfish sponges, is not known, given similarity of developmental genetic repertoires shared by all these taxa. Here, we show that a core set 18 microRNAs (miRNAs), non‐coding RNA molecules negatively regulate expression protein‐coding genes, are found only protostomes deuterostomes sponges or cnidarians. Because many miRNAs expressed specific tissues and/or organs,...
Abstract Purpose: MicroRNA (miRNA) expression profiles improve classification, diagnosis, and prognostic information of malignancies, including lung cancer. This study uncovered unique growth-suppressive miRNAs in Experimental Design: miRNA arrays were done on normal tissues adenocarcinomas from wild-type proteasome degradation-resistant cyclin E transgenic mice to reveal repressed Real-time semiquantitative reverse transcription-PCR as well situ hybridization assays validated these...
Abstract Introduction: Pancreatic ductal adenocarcinoma (PDAC) is a lethal malignancy. Diagnosis and management of PDAC are hampered by the absence sensitive specific disease biomarkers. MicroRNAs (miRNA) noncoding regulatory RNAs involved in initiation progression human cancers. In this study, we sought to determine whether miR-10b could serve as biomarker for PDAC. Experimental Design: miRNA expression was characterized fluorescence-based situ hybridization using locked nucleic...
Abstract Modulating the activity of miRNAs provides opportunities for novel cancer interventions. However, low bioavailability and poor cellular uptake are major challenges delivering miRNA mimetics specifically to tumor cells. Here, we took advantage spontaneous enhanced endocytic ovarian cancer-associated dendritic cells (DC) selectively supplement immunostimulatory miR-155. In vivo processing nanoparticles carrying oligonucleotide duplexes mimicking bulged structure endogenous pre-miRNA...
Abstract Purpose: High-throughput profiling experiments have linked altered expression of microRNAs (miRNA) to different types cancer. Tumor tissues are a heterogeneous mixture not only cancer cells, but also supportive and reactive tumor microenvironment elements. To clarify the clinical significance miRNA in solid tumors, we developed sensitive fluorescence-based situ hybridization (ISH) method visualize accumulation within individual cells formalin-fixed, paraffin-embedded tissue...
SUMMARY Phylogenetic analyses based on gene sequences suggest that acoel flatworms are not members of the phylum Platyhelminthes, but instead most basal branch triploblastic bilaterians. Nonetheless, this result has been called into question. An alternative test is to use qualitative molecular markers should, in principle, exclude possibility convergent (homoplastic) evolution unrelated groups. microRNAs (miRNAs), noncoding regulatory RNA molecules under intense stabilizing selection, a...
Here, we describe the synthesis, characterization and in vitro vivo performance of a series tantalum oxide (TaOx) based nanoparticles (NPs) for computed tomography (CT). Five distinct versions 9-12 nm diameter silane coated TaOx nanocrystals (NCs) were fabricated by sol-gel method with varying degrees hydrophilicity or without fluorescence, highest reported Ta content to date (78%). Highly hydrophilic NCs left bare evaluated mice micro-CT full body vasculature, where following intravenous...
Abstract UBE1L is the E1-like ubiquitin-activating enzyme for IFN-stimulated gene, 15-kDa protein (ISG15). The UBE1L-ISG15 pathway was proposed previously to target lung carcinogenesis by inhibiting cyclin D1 expression. This study extends prior work reporting that promotes a complex between ISG15 and inhibited but not other G1 cyclins. Transfection of deconjugase, ubiquitin-specific 18 (UBP43), antagonized UBE1L-dependent inhibition ISG15-cyclin conjugation. A lysine-less species resistant...
Genetic analysis of TP63 indicates that ΔNp63 isoforms are required for preservation regenerative stasis within diverse epithelial tissues. In squamous carcinomas, is commonly amplified, and ΔNp63α confers a potent survival advantage. Genome-wide occupancy studies show promotes bidirectional target gene regulation by binding more than 5,000 sites throughout the genome; however, subset targets mediating discreet activities remains unclear. We report activates bone morphogenic proteins (BMP)...
Perivascular adipose tissue (PVAT) regulates vascular function due to its capacity synthesize vasoactive products and mechanical properties. PVATs most abundant cells are adipocytes, their populations maintained by the maturation of adipocyte progenitor (APC), which may play a pivotal role in pathogenesis cardiovascular diseases. However, distribution APC within PVAT depots, potential variation spatial location, influence sex age on abundance remain unknown. We hypothesize that is affected...
Abstract This essay focuses on the ethical considerations and implications of providing a universal multi-cancer screening test as best approach to reduce societal cancer burden in society with limited funds, resources, infrastructure. With 1.9 million diagnoses each year United States, 86% all cancers diagnosed individuals over age 50, tools approved for only four types (breast, cervical, colorectal, lung cancer), it seems that detect most early is easy administer, accurate cost-effective,...