A5102856866- ATP Synthase and ATPases Research
- Mitochondrial Function and Pathology
- Cardiomyopathy and Myosin Studies
- Cancer-related molecular mechanisms research
- Cardiovascular Function and Risk Factors
- T-cell and B-cell Immunology
- Chemical Synthesis and Analysis
- Cancer-related gene regulation
- RNA modifications and cancer
- Receptor Mechanisms and Signaling
- Multicomponent Synthesis of Heterocycles
- Synthesis and Biological Evaluation
- MicroRNA in disease regulation
- Cancer Mechanisms and Therapy
- interferon and immune responses
- Pleural and Pulmonary Diseases
- Chemokine receptors and signaling
- Myofascial pain diagnosis and treatment
- Sympathectomy and Hyperhidrosis Treatments
- Transplantation: Methods and Outcomes
- Nephrotoxicity and Medicinal Plants
- Ferroptosis and cancer prognosis
- Neurofibromatosis and Schwannoma Cases
- Soft tissue tumor case studies
- Cancer Immunotherapy and Biomarkers
Third Affiliated Hospital of Sun Yat-sen University
2017-2025
Rega Institute for Medical Research
2020-2024
KU Leuven
2020-2024
Sun Yat-sen University
2014-2023
Alibaba Group (China)
2020
Abstract The human CC chemokine receptor 8 (CCR8) is specifically expressed on tumor-infiltrating regulatory T cells (TITRs) and a promising drug target for cancer immunotherapy. However, the role of CCR8 signaling in TITR biology effectiveness small molecule antagonists as TITR-targeting immunotherapy remain subjects ongoing debate. In this work, we generated novel cellular model TITRs by culturing peripheral blood mononuclear cell-derived medium containing tumor cell-conditioned medium,...
MiR-145 has been implicated in the progression of non-small cell lung cancer (NSCLC); however, its exact mechanism is not well established. Here, we report that miR-145 expression decreased NSCLC lines and tumor tissues this low level associated with DNA methylation. methylation was correlated a more aggressive phenotype poor survival time, as shown by Kaplan-Meier analysis. Additional multivariate Cox regression analysis indicated an independent prognostic factor for patients NSCLC....
Non-small cell lung cancer (NSCLC) is a major type of human and the primary cause cancer-associated cases mortality worldwide. Phosphatase tensin homolog (PTEN) potent tumor suppressor gene in various types. The aim current study was to explore role PTEN its associated regulatory mechanisms NSCLC. Firstly, expression detected using western blotting variety NSCLC lines. results revealed that compared with normal control cells, levels were significantly decreased lines (P<0.01). Short hairpin...
Large tumor suppressor kinase 2 (Lats2) is a member of the Hippo pathway, critical regulator organ size. Since Lats2 activity may trigger mitochondrial dysfunction, key pathogenic factor in acute myocardial infarction (AMI), this study sought to investigate whether deletion confers cardioprotection AMI. AMI was induced cardiomyocyte-specific knockout (Lats2Cko) and control (Lats2flox) mice. Twenty-eight days after surgery, performance homeostasis were impaired Lats2floxmice. In contrast,...
Abstract Background The crosstalk of purine biosynthesis and metabolism exists to balance the cell energy production, proliferation, survival cytoplasmic environment stability, but disorganized mechanics with respect developing heart failure (HF) is currently unknown. Methods We conducted a multi-omics wide analysis, including microarray-based transcriptomes, full spectrum metabolomics chronic HF. Based on expression profiling by array, we applied bioinformatics platform quantifiable...
This study applied a complex bioinformatics analysis to explore the hub regulators and immune network further elucidate molecular mechanisms of lung adenocarcinoma (LUAD) regulation.LUAD immunological microenvironment features microenvironment-related differential expression genes (DEGs) were identified by ESTIMATE algorithm linear models for microarray analyses (LIMMA), respectively. CIBERSORT Igraph algorithms construct LUAD-related immunocyte infiltration regulatory network. Kaplan-Meier...
The human CC chemokine receptor 8 (CCR8) is a promising drug target for cancer immunotherapy and autoimmune disease. Besides viral chemokines, previous studies revealed diverse classes of CCR8-targeting small molecules. We characterized selection these CCR8 ligands (hCCL1, vCCL1, ZK756326, AZ6; agonists naphthalene-sulfonamide-based antagonist), in vitro cell-based assays (hCCL1AF647 binding, calcium mobilization, cellular impedance, cell migration, β-arrestin 1/2 recruitment), used...
Background: Tetralogy of Fallot (TOF) is the most common cyanotic heart disease. However, association cardiac metabolic reprogramming changes and underlying molecular mechanisms in TOF-related chronic myocardial hypoxia damage are still unclear. Methods: In this study, we combined microarray transcriptomics analysis with liquid chromatography tandem-mass spectrometry (LC–MS/MS) spectrum metabolomics to establish that occurs response damage. Two Gene Expression Omnibus (GEO) datasets,...
Tetralogy of Fallot (TOF) belongs to cyanotic heart damage, which is the most common in clinic. In chronic myocardial hypoxia injury related TOF, potential molecular mechanism cardiac energy metabolism remains unclear.In our study, microarray transcriptome analysis and metabonomics methods were used explore pathway during injury. The gene expression omnibus (GEO) dataset GSE132176 was obtained for analyzing metabolic pathways. clinical samples (right atrial tissues) septal defect (ASD) TOF...
Desmoid-type fibromatosis (DF), also known as deep or desmoid tumor, is an extremely rare neoplasm that develops from fascia and musculoaponeurotic tissue. These tumors are characterized by slow progressive growth, local invasion, recurrence after surgical excision, but they lack metastatic potential. DF accounts for 3.5% of all fibrous tumors, with annual incidence approximately 2-4/million. Until now, only a small number cases have been found in the chest wall. Herein, we present case wall...
Background: The etiology and pathogenesis of idiopathic pulmonary fibrosis (IPF) remain unclear, the early detection treatment are vital to prognosis IPF patients. It’s necessary investigate effect thalidomide on gene promoter activation connective tissue growth factor (CTGF) induced by transforming factor-β1 (TGF-β1) in human embryonic lung fibroblast (HELF). Methods: vector pGL3-CTGFP containing CTGF was constructed transfected into HELF cells. We used different TGF-β1 (0, 2.5, 5, 10 20...
Objective To investigate the mechanism of microRNA (miRNA, miR)-145 in modulating capacity proliferation and invasion esophageal squamous cell carcinoma (ESCC). Methods Real-time quantitative reverse transcriptase-polymerase chain reaction (RT-qPCR) was used to detect expression miR-145 phospholipase C epsilon 1 (PLCE1) mRNA. PLCE1 protein level evaluated by Western blotting. The potential target gene predicted targetscan software. Luciferase reporter assay validate binding site...
Objective To explore the application value of thoracoscopic pleural lavage for empyema after liver transplantation (LT). Methods Clinical data 5 patients who underwent LT in Third Affiliated Hospital Sun Yat-sen University from October 2002 to 2013 were analyzed retrospectively. All males with age ranging 42 56 years old and median 51 old. The informed consents all obtained local ethical committee approval had been received. received under endotracheal general anesthesia. thoracoscope...