A5068469239- RNA Research and Splicing
- Genomics and Chromatin Dynamics
- Muscle Physiology and Disorders
- RNA and protein synthesis mechanisms
- RNA modifications and cancer
- Cancer Genomics and Diagnostics
- Single-cell and spatial transcriptomics
- Ubiquitin and proteasome pathways
- Antimicrobial agents and applications
- CRISPR and Genetic Engineering
- Alzheimer's disease research and treatments
- Antimicrobial Peptides and Activities
- Cancer-related molecular mechanisms research
- Plant Molecular Biology Research
- Animal Disease Management and Epidemiology
- Protein Degradation and Inhibitors
- Bacterial Genetics and Biotechnology
- 3D Printing in Biomedical Research
- Enzyme Production and Characterization
- Neuroscience and Neural Engineering
- interferon and immune responses
- Cholangiocarcinoma and Gallbladder Cancer Studies
- Mitochondrial Function and Pathology
- Data-Driven Disease Surveillance
- Monoclonal and Polyclonal Antibodies Research
Zhejiang University
2021-2025
Sir Run Run Shaw Hospital
2024-2025
University of South Florida
2017-2024
Boston Medical Center
2023-2024
Shandong Provincial Hospital
2024
Shandong University
2024
Massachusetts General Hospital
2019-2024
First Affiliated Hospital Zhejiang University
2024
Anhui University
2024
Boston University
2018-2023
DNA sequence is a major determinant of the binding specificity transcription factors (TFs) for their genomic targets. However, eukaryotic cells often express, at same time, TFs with highly similar motifs but distinct in vivo Currently, it not well understood how seemingly identical achieve unique specificities vivo. Here, we used custom protein-binding microarrays to analyze TF putative sites context. Using yeast Cbf1 and Tye7 as our case studies, found that these bHLH (i.e., E-boxes) are...
Significance Genomes provide an abundance of putative binding sites for each transcription factor (TF). However, only small subsets these potential targets are functional. TFs the same protein family bind to target that very similar but not identical. This distinction allows closely related regulate different genes and thus execute distinct functions. Because nucleotide sequence core motif is often sufficient identifying a genomic target, we refined description TF by introducing combination...
Prospects for specific immune intervention in T cell-mediated autoimmune disease via anti-idiotypic regulation depend on the degree of diversity responder cell antigen receptor repertoire. A highly heterogenous response against self epitopes offers little chance such regulation. We report here that Lewis rat experimental allergic encephalomyelitis, generally considered to be a model human multiple sclerosis, is caused by cells use limited set TCR V genes. have cloned alpha and beta chain...
Abstract The relationship between amyloid-β (Aβ) species and tau pathology in Alzheimer’s disease (AD) is not fully understood. Here, we provide direct evidence that Aβ42/40 ratio, total Aβ level, plays a critical role inducing neurofibrillary tangles (NTFs) human neurons. Using 3D-differentiated clonal neural progenitor cells (hNPCs) expressing varying levels of amyloid β precursor protein (APP) presenilin 1 (PS1) with AD mutations, show pathogenic accumulation aggregation are tightly...
Long-term implantation of biomedical electronics into the human body enables advanced diagnostic and therapeutic functionalities. However, most long-term resident devices require invasive procedures for as well a specialized receiver communication. Here, gastric electronic (GRE) system that leverages anatomical space offered by environment to enable residence an orally delivered platform such within is presented. The GRE capable directly interfacing with portable consumer personal through...
Abstract Identifying pathogenic variants from the vast majority of nucleotide variation remains a challenge. We present method named Multimodal Annotation Generated Pathogenic Impact Evaluator (MAGPIE) that predicts pathogenicity multi-type variants. MAGPIE uses ClinVar dataset for training and demonstrates superior performance in both independent test set multiple orthogonal validation datasets, accurately predicting variant pathogenicity. Notably, performs best rare highly imbalanced...
Abstract Triple-negative breast cancer (TNBC) is traditionally considered a glycolytic tumor with poor prognosis while lacking targeted therapies. Here we show that high expression of dihydrolipoamide S-succinyltransferase (DLST), tricarboxylic acid (TCA) cycle enzyme, predicts overall and recurrence-free survival among TNBC patients. DLST depletion suppresses growth induces death in subsets human cell lines, which are capable utilizing glutamine anaplerosis. Metabolomics profiling reveals...
The major encephalitogenic epitope for Lewis rats is the 72-89 sequence of guinea pig basic protein (GP-BP) or rat (Rt-BP). T cells responsive to this are I-A restricted and preferentially express V alpha 2:V beta 8 gene combination in their TCR. In work, we describe first time delayed appearance specific additional discrete determinant BP, nonencephalitogenic 55-68 GP-BP by I-A, 87-99 Rt-BP I-E. TCR was expressed both S72-89 S87-99 cell specificities but not 44-68-specific cells. This...
Gene fusions can play important roles in tumor initiation and progression. While fusion detection so far has been from bulk samples, full-length single-cell RNA sequencing (scRNA-seq) offers the possibility of detecting gene at level. However, scRNA-seq data have a high noise level contain various technical artifacts that lead to spurious discoveries. Here, we present computational tool, scFusion, for based on scRNA-seq. We evaluate performance scFusion using simulated five real datasets...
The emerging antibiotic resistance has been named by the World Health Organization (WHO) as one of top 10 threats to public health. Notably, methicillin-resistant Staphylococcus aureus (MRSA) and vancomycin-resistant Enterococcus faecalis (VREF) are designated serious threats, whereas Clostridioides difficile (C. difficile) is recognized most urgent human health unmet medical need. Herein, they report design application novel biodegradable polymers - lipidated antimicrobial guanidinylate...
Basic peptides covalently linked to nucleic acids, or chemically modified enable the insertion of such a conjugate into bacteria grown in liquid medium and mammalian cells tissue culture. A unique peptide, derived from human T cells, has been employed chemical synthesis make with morpholino oligonucleotide. This new is at least 10- 100-fold more effective than previous used altering phenotype host if external guide sequence methodology these experiments. Bacteria target genes expressing...
A class of small dimeric cyclic guanidine derivatives was designed to display potent and broad spectrum antibacterial activity.
Facioscapulohumeral muscular dystrophy (FSHD) is caused by the loss of repression at D4Z4 locus leading to aberrant double homeobox 4 (DUX4) expression in skeletal muscle. Activation this early embryonic transcription factor results its target genes causing muscle fiber death. Although progress toward understanding signals driving DUX4 has been made, factors and pathways involved transcriptional activation gene remain largely unknown. Here, we describe identification characterization p38α as...
We present SpliceTransformer (SpTransformer), a deep-learning framework that predicts tissue-specific RNA splicing alterations linked to human diseases based on genomic sequence. SpTransformer outperforms all previous methods prediction. Application approximately 1.3 million genetic variants in the ClinVar database reveals account for 60% of intronic and synonymous pathogenic mutations, occur at different frequencies across tissue types. Importantly, match their clinical manifestations...
Abstract Background COVID-19, the disease caused by highly infectious and transmissible coronavirus SARS-CoV-2, has quickly become a morbid global pandemic. Although impact of SARS-CoV-2 infection in children is less clinically apparent, collecting high-quality biospecimens from infants, children, adolescents standardized manner during COVID-19 pandemic essential to establish biologic understanding pediatric population. This biorepository enables centers world-wide collect samples uniformly...
The association between sex and outcome after endovascular thrombectomy of acute ischemic stroke is unclear. aim this study was to compare the clinical safety outcomes men women treated with in late 6-to-24-hour window period.
The expression of gene products in bacteria can be inhibited by the use RNA external guide sequences (EGSs) that hybridize to a target mRNA. Endogenous RNase P cleaves mRNA complex, making it inactive. EGSs participate this biochemical reaction as data presented here show. They promote cleavage at expected site and sometimes other secondary sites. Higher-order structure must affect these reactions if does not occur defined site, which has been determined techniques based on their ability...