A5002671914- T-cell and B-cell Immunology
- Immune Cell Function and Interaction
- Immunotherapy and Immune Responses
- Influenza Virus Research Studies
- Service-Oriented Architecture and Web Services
- Web Application Security Vulnerabilities
- Access Control and Trust
- Systemic Lupus Erythematosus Research
- Immune Response and Inflammation
- Political Influence and Corporate Strategies
- Digital and Cyber Forensics
- Monoclonal and Polyclonal Antibodies Research
- Security and Verification in Computing
- Radiomics and Machine Learning in Medical Imaging
- Studies on Chitinases and Chitosanases
- Respiratory viral infections research
- Insect symbiosis and bacterial influences
- Mobile Agent-Based Network Management
- SARS-CoV-2 and COVID-19 Research
- AI in cancer detection
- COVID-19 Clinical Research Studies
- Invertebrate Immune Response Mechanisms
- Peptidase Inhibition and Analysis
- Scientific Computing and Data Management
- Business Process Modeling and Analysis
Duke University
2018-2025
Bayer (United States)
2021-2024
University of Alabama at Birmingham
2014-2023
St. Vincent's Birmingham
2023
University of Cambridge
2022
Monash University
2010
Research Applications (United States)
1982-1990
American Meteorological Society
1982-1987
Ford Motor Company (France)
1984
The coronavirus disease 2019 (COVID-19) pandemic has highlighted the urgent need for effective prophylactic vaccination to prevent spread of severe acute respiratory syndrome 2 (SARS-CoV-2). Intranasal is an attractive strategy COVID-19 as nasal mucosa represents first-line barrier SARS-CoV-2 entry. current intramuscular vaccines elicit systemic immunity but not necessarily high-level mucosal immunity. Here, we tested a single intranasal dose our candidate adenovirus type 5-vectored vaccine...
Although B cells expressing the IFNγR or IFNγ-inducible transcription factor T-bet promote autoimmunity in Systemic Lupus Erythematosus (SLE)-prone mouse models, role for IFNγ signaling human antibody responses is unknown. We show that elevated levels of SLE patients correlate with expansion IgDnegCD27negCD11c+CXCR5neg (DN2) pre-antibody secreting cell (pre-ASC) subset. demonstrate naïve form T-bethi pre-ASCs following stimulation either Th1 IFNγ, IL-2, anti-Ig and TLR7/8 ligand IL-21...
Abstract Epigenetic remodeling is required during B cell differentiation. However, little known about the direct functions of epigenetic enzymes in Ab-secreting cells (ASC) vivo. In this study, we examined ASC differentiation independent T help and germinal center reactions using mice with inducible or cell–specific deletions Ezh2. Following stimulation influenza virus LPS, Ezh2-deficient poorly proliferated inappropriately maintained expression inflammatory pathways, cell–lineage...
The coronavirus disease 2019 (COVID-19) pandemic has highlighted the urgent need for effective preventive vaccination to reduce burden and spread of severe acute respiratory syndrome (SARS) 2 (SARS-CoV-2) in humans. Intranasal is an attractive strategy prevent COVID-19 as nasal mucosa represents first-line barrier SARS-CoV-2 entry before viral lung. Although vaccine development rapidly progressing, current intramuscular vaccines are designed elicit systemic immunity without conferring...
ICOS is a T-cell costimulatory receptor critical for Tfh cell generation and function. However, the role of in Tfr differentiation remains unclear. Using Foxp3-Cre–mediated knockout (ICOS FC) mice, we show that deficiency Treg-lineage cells drastically reduces number during GC reactions but has minimal impact on conventional Treg cells. Single-cell transcriptome analysis Foxp3 + at an early stage reaction suggests normally inhibits Klf2 expression to promote follicular features including...
Understanding how cellular pathways interact is crucial for treating complex diseases like cancer, yet our ability to map these connections systematically remains limited. Individual gene-gene interaction studies have provided insights 1,2 , but they miss the emergent properties of working together. To address this challenge, we developed a multi-gene approach pathway mapping and applied it CRISPR data from Cancer Dependency Map 3 . Our analysis electron transport chain revealed certain...
Abstract Ab-secreting cells (ASC) or plasma are essential components of the humoral immune system. Although Abs different isotypes have distinct functions, it is not known if ASC that secrete each isotype also distinct. downregulate their surface BCR upon differentiation, hindering analyses couple information to other molecular characteristics. In this study, we developed a methodology using fixation, permeabilization, and intracellular staining coupled with cell sorting reversal cross-links...
Abstract Autoreactive B cells are associated with the development of several autoimmune diseases, including systemic lupus erythematosus and rheumatoid arthritis. The low frequency these represents a major barrier to their analysis. Ag tetramers prepared from linear epitopes represent promising strategy for identification small subsets Ag-reactive immune cells. This is challenging given requirement validation complexity autoantibody responses, broad spectrum specificities contribution...
The monoclonal antibody FMC7 delineates a subpopulation of B lymphocytes in normal blood. Expression the antigen recognized by appears to be maturation-linked, and it serves distinguish different types cell leukemia. data presented here indicate that is protein integrated membrane able interact with cytoskeleton. rapidly synthesized turned over, not cycle-dependent, relatively resistant changes induced culture presence phorbol ester.
Abstract Seasonal influenza vaccination elicits hemagglutinin (HA)-specific CD27 + memory B cells (Bmem) that differ in expression of T-bet, BACH2 and TCF7. T-bet hi lo TCF7 Bmem are transcriptionally similar to effector-like while exhibit stem-like central properties. do not express plasma cell-specific transcription factors but transcriptional, epigenetic, metabolic functional changes poise the for antibody production. Consistent with these changes, D7 HA intracellular immunoglobulin...
Abstract Long-lived T-dependent B cell responses fail to develop during persistent infection of mice with Borrelia burgdorferi, the causative agent Lyme disease, raising questions about induction and/or functionality anti–B. burgdorferi adaptive immune responses. Yet, a lack reagents has limited investigations into B. burgdorferi–specific T and cells. We attempted two approaches track burgdorferi–induced CD4 First, mutant was generated an influenza hemagglutinin (HA) peptide, HA111–119,...