Effects of Akt1 gene knockout on pain behaviour induced by chronic constriction injury of sciatic nerve in mice

• DOI: 10.3760/cma.j.issn.1674-6554.2012.01.006
• Publication Date: 2014-03-20
• Authors: Jinhua Bo; Xiaoping Gu; Xiaofeng Sun; Jing Zhang
• Keywords: Knockout mouse; Gene knockout; Constriction

Objective To investigate the effects of Akt3 gene knockout on neuropathic pain behaviors induced by chronic constriction injury sciatic nerve (CCI).

Methods Experiment was divided into two groups: group(Akt3-/-, n=12), wild type group(WT, n=12). Randomly numbered, right mice were received operation injury. Paw withdrawal mechanical threshold (PWMT) and paw thermal latency (PWTL) tested day 1 before 1, 3, 5, 7, 10, 14, 17, 21 after CCI.

Results The basic values PWMT(right: (1.09±0.20)g, (1.17±0.22)g; left: (1.17±0.15)g, (1.22±0.23)g, P>0.05) PWTL(right: (6.18±1.11)s, (6.20±1.25)s; (5.82±0.91)s, (5.92±1.71)s, had no statistically significant differences between groups.On operation, compared with values, PWMT PWTL in both Akt3-/- group WT decreased significantly (P 0.05). However, to left paw, groups obviously lower(P<0.05).

Conclusion The CCI increased mice.

Key words: Akt3; Gene knock out; Neuropathic