Effect of valsartan and fluvastatin on the expression of glycogen synthase kinase-3β in retina of diabetic rat

• DOI: 10.3760/cma.j.issn.2095-0160.2012.02.005
• Publication Date: 2012-02-10
• Authors: Qin-jin Chen; Hui-long Lu; Qi Yang
• Keywords: Fluvastatin

Background Glycogen synthase kinase-3β (GSK-3β) plays an important role in glucose metabolism,and it may be affect the occurrence of diabetic retinopathy(DR). Objective The present study was to investigate effect valsartan and fluvastatin on expression GSK-3β retina diabetes rat model.Methods Diabetes mellitus models were induced by intrapenetoneal injection streptozotocin(STZ) 47 clean Sprague-Dawley(SD) rats then randomedly divided into 4 groups.Ten other normal served as control group.Sodium carboxy methyl cellulose solution,valsartan,fluvastatin,valsartan+fluvastatin sodium solution given oral once per day for 12 weeks respectively group ( n =12),valsartan =12 ),fluvastatin =11 ),valsartau + ) group.Twelve after administration drugs,blood measured compared among various groups,and p-GSK-3β Ser-9 protein quantified located Western blot immunohistochemistry,respectively. Results Twelve use drugs,the level blood was(5.28±0.30),(26.08±3.33 ),(26.03 ±2.66 ),(25.90± 2.86 ),(25.99 ± 2.14 mmol/L group,diabetes group,valsartan,fluvastatin,valsartan group,respectively,showing a significant difference 5 groups F =110.74,P＜0.01 ).Western showed that grey value /β-actin higher than group(2.774±0.139 vs 1.927±0.111,q =15.79,P＜0.01 ),and valsartan,fluvastatin,valsartan+fluvastatin lower 1.895 ±0.090,2.051 0.113,1.537 0.071 2.774 0.139 q =1 3.69,13.48,23.06,P ＜ 0.01 ).The (Ser-9)/β-actin valsartan+fluvastatin declined comparison with =6.67,9.58,P＜0.01 ).Immunohistochemistry p-GSK-3β(Ser-9) expressed all over retinal layers obviously ganglion cell layer(GCL) group.But significantly group.The (Ser-9) attenuated both further group. Conclusions is overexpressed GCL rat.Both can inhibit even getting stronger when they combined.

Key words: Glycogen kinase-3β; Diabetes mellitus/experimental; Retina