Combinatorial protective effects of α-melanocyte-stimulating hormone and carboxymethylcellulose on ocular surface in dry eye rat models

• DOI: 10.3760/cma.j.issn.2095-0160.2017.06.004
• Publication Date: 2017-06-10
• Authors: Zexia Dou; Yusha Ru; Huijuan Liu; Xiaoyu Zeng; Meng Zhu; Zhongxiu Gu; Yan Zhang
• Keywords: Subcutaneous injection; Melanocyte-stimulating hormone; Eosin; Eye drop; Scopolamine Hydrobromide; Sodium hyaluronate

Background Reasearches showed that α-melanocyte-stimulating hormone (α-MSH) inhibits inflammation and ameliorates the ocular surface abnormalities in a scopolamine-induced dry eye rat model, managing effect of sodium carboxymethylcellulose (CMC) on eyes also has been determined.However, whether α-MSH can enhance therapeutic effects CMC remains to be investigated.

Objective This study was investigate protective combined with model.

Methods Sixty clean female Wistar rats were randomly divided into normal control group, model NaCl group α-MSH+ 10 for each group.The models established by subcutaneous injection scopolamine hydrobromide at 9: 00, 12: 15: 00 18: per day 28 days.0.9% solution, 1×10-3 mg/ml 0.5% drop, solution topically administered twice (8: 17: 00) since initial modeling according grouping.Shirmer Ⅰ test (SⅠt), breakup time tear film (BUT) corneal fluorescence staining performed before 7, 14, 21, days after application drugs.At following administration drugs, eyeballs collected.Hemotoxylin eosin employed examine morphology corneas, periodic acid schiff (PAS) used count conjunctival goblet cells.This protocol approved Experimental Animal Ethic Committee Tianjin Medical University(SYXK 2009-0001), use care complied ARVO Statement.

Results The SⅠt BUT values significantly reduced, scores increased over (all P 0.05). At 14 21 intervention, (4.800±0.789), (4.100±0.516) (4.300±0.856)mm which considerably higher than (2.875±0.719), (2.375±0.619) (2.532±0.957)mm P<0.01). 7 (4.938±1.843) seconds (5.000±1.491) (3.250±1.000) (both The lower lowest score P<0.05). thickening epithelial layer, edema arrangement disorder stroma found group; while slight cell proliferation exhibited hemotoxylin staining.PAS number cells much more P<0.01).

Conclusions The sole or alleviates damage morphological abnormality certain extent, combination generates effective protection comparison CMC.The early drugs plays an improvement role secretion stability eyes.

Key words: Dry eyes/drug therapeutic; Ocular surface; Protection; Sodium carboxymethylcellulose; α-Melanocyte-stimulating hormone; Disease models; Rats,