[Anti-angiogenesis effect of G4PAMAM/VEGFASODN on breast cancer in vitro].

• DOI: 10.3785/j.issn.1008-9292.2008.06.012
• Publication Date: 2008-11-01
• Authors: Xinhan Zhao; Li Li; Zhiyu Wang; Ling-xiao Znang; Gaili An
• Keywords: Lipofectamine

To investigate the effect of G4PAMAM/VEGFASODN compound on expression VEGF and mRNA in MDA-MB-231 breast cancer cells growth inhibition vascular endothelial cells.The diameter granule was measured by transmission electron microscopy, its stability under different pH also observed. The efficiency transfection vitro detected flow cytometer positively transfected were laser scanning confocal microscope. survival rate inhibitory determined MTT assay. protein immunohistochemical method RT-PCR.The granules about 10 nm it arranged as homogeneous netlike. Under 5-10 presented highly stable no dissociation observed with charge ratios. 48-hour ratio 1:40 significantly higher than that lipofectamine group. None products each group showed cell toxicity. staining cytoplasm after G4PAMAM/ASODN weakened markedly, positive decreased. Meanwhile, decreased.With good rate, can be a promising new nanometer vector gene transfer system. inhibit specifically efficiently, which may used for vivo animal experiment.