Expression and significance of SDF-1/CXCR4 in alkali-burned cornealtissue in mouse

• DOI: 10.3969/j.issn.1003-0808.2010.02.010
• Publication Date: 2010-02-10
• Authors: Lianghong Peng; Lin Liu
• Keywords: Corneal Neovascularization

Background Recent research showed that stromal cell-derived factor-1 (SDF-1) plays critical role in mediating inflammation,metastasis of tumour and neovascularization tumour.However,There is still no report about the effects SDF-1 on alkali-burn-induced corneal (CNV).Objective The aim this study attempts to investigate chemokine receptor CXCR4 alkali-induced mice.Methods Alkali-induced-corneal animal models were constructed 15 eyes clean C57/BL mice by placing filter paper with 1 mol/L NaOH central cornea for 10 seconds.The animals sacrificed specimens obtained 3,7,14 days after alkali burn cornea.The expression SDF-1/CXCR4 protein tissues was detected immunohistochemistry Western blot,and mRNA reverse transcription PCR (RT-PCR).Results absent expressed faintly stained normal level significantly enhanced different time points comparison control RT-PCR(P＜0.05),and same trend seen proteins blot (P＜0.05).The peaked seventh day began decline fourteenth but higher than level.Conclusion indicates an important formation new vessel model.

Key words: alkali burn; corneal neovascularization; stromal factor-1; CXCR4