A5022783080- Advanced Fluorescence Microscopy Techniques
- Innovative Microfluidic and Catalytic Techniques Innovation
- Biotin and Related Studies
- Bacteriophages and microbial interactions
- Systemic Lupus Erythematosus Research
- Genomics and Phylogenetic Studies
- Inflammasome and immune disorders
- Alzheimer's disease research and treatments
- Monoclonal and Polyclonal Antibodies Research
- Photosynthetic Processes and Mechanisms
- Protein Structure and Dynamics
- Adenosine and Purinergic Signaling
- Endoplasmic Reticulum Stress and Disease
- Pancreatic function and diabetes
- Rheumatoid Arthritis Research and Therapies
- Cell Image Analysis Techniques
Bioscience (China)
2024
University of Cambridge
2015-2016
The characterization of the aggregation kinetics protein amyloids and structural properties ensuing aggregates are vital in study pathogenesis many neurodegenerative diseases discovery therapeutic targets. In this article, we show that fluorescence lifetime synthetic dyes covalently attached to amyloid proteins informs on clusters formed both vitro cells. We demonstrate mechanism behind such a "lifetime sensor" is based self-quenching it offers good dynamic range report various stages...
Endoplasmic reticulum (ER) lumenal protein thiol redox balance resists dramatic variation in unfolded load imposed by diverse physiological challenges including compromise the key upstream oxidases. Lumenal calcium depletion, incurred during normal cell signaling, stands out as a notable exception to this resilience, promoting rapid and reversible shift towards more reducing poise. Calcium depletion induced ER alterations are relevant conditions associated with such response of pancreatic...
FRET is widely used for the study of protein-protein interactions in biological samples. However, it difficult to quantify both efficiency (E) and affinity (Kd) molecular interaction from intermolecular signals samples unknown stoichiometry. Here, we present a method simultaneous quantification complete set parameters, including fractions bound donors acceptors, local protein concentrations, dissociation constants, each image pixel. The makes use fluorescence lifetime information donor...
Most fluorescent proteins exhibit multiexponential fluorescence decays, indicating a heterogeneous excited state population. FRET between should therefore involve multiple energy transfer pathways. We recently demonstrated the pathways EGFP and mCherry (mC), upon dimerization of 3-phosphoinositide dependent protein kinase 1 (PDK1), to be highly restricted. A mechanism for restriction based on unfavorable κ2 orientation factor arising from differences in donor-acceptor transition dipole...
DNA sequencers have become increasingly important research and diagnostic tools over the past 20 years. In this study, we developed a single-molecule desktop sequencer, GenoCare 1600 (GenoCare), which utilizes amplification-free library preparation two-color sequencing-by-synthesis chemistry, making it more user-friendly compared with previous sequencing platforms for clinical use. Using platform, sequenced an Escherichia coli standard sample achieved consensus accuracy exceeding 99.99%. We...