A5077958810- Platelet Disorders and Treatments
- Neurogenesis and neuroplasticity mechanisms
- Cell Adhesion Molecules Research
- Neuroinflammation and Neurodegeneration Mechanisms
- Neuroscience and Neuropharmacology Research
- Retinal Development and Disorders
- Blood groups and transfusion
- Nerve injury and regeneration
- Monoclonal and Polyclonal Antibodies Research
- Hematopoietic Stem Cell Transplantation
- Cardiac Valve Diseases and Treatments
- Spinal Cord Injury Research
- Heparin-Induced Thrombocytopenia and Thrombosis
- Hemophilia Treatment and Research
- Blood Coagulation and Thrombosis Mechanisms
- Advanced Fluorescence Microscopy Techniques
- Elasticity and Material Modeling
- Immune cells in cancer
- Cardiac Ischemia and Reperfusion
- Neutrophil, Myeloperoxidase and Oxidative Mechanisms
- Cell Image Analysis Techniques
- Cardiovascular Function and Risk Factors
- Axon Guidance and Neuronal Signaling
- Chemokine receptors and signaling
- Protein Kinase Regulation and GTPase Signaling
University of Colorado Denver
2020-2025
University of Colorado Anschutz Medical Campus
2019-2024
University of California, Los Angeles
2018-2021
Florida Agricultural and Mechanical University
2005-2010
Children's Hospital of Philadelphia
2001-2008
University of Pennsylvania
1999-2003
Medical College of Wisconsin
2003
Robarts Clinical Trials
2003
Tel Aviv Sourasky Medical Center
1999
Sheba Medical Center
1999
Myelin loss induces neural dysfunction and contributes to the pathophysiology of neurodegenerative diseases, injury conditions, aging. Because remyelination is often incomplete, better understanding endogenous developing therapies that restore function are clinical imperatives. Here, we use in vivo two-photon microscopy electrophysiology study dynamics therapeutic-induced cortical functional recovery after cuprizone-mediated demyelination mice. We focus on visual pathway, which uniquely...
Two geometrically identical models of the leaflets a bicuspid bioprosthetic heart value have been constructed using finite elements. The boundary conditions applied to were also but linear material model has used in one and nonlinear elastic other. full-scale contained 2600 Belytschko-Lin-Tsai shell elements which allowed variation stress through thickness leaflet be modelled. A time-varying, spatially-uniform pressure differential was across their behaviour during complete cardiac cycle....
Oligodendrocyte loss in neurological disease leaves axons vulnerable to damage and degeneration, activity-dependent myelination may represent an endogenous mechanism improve remyelination following injury. Here, we report that while learning a forelimb reach task transiently suppresses oligodendrogenesis, it subsequently increases OPC differentiation, oligodendrocyte generation, retraction of pre-existing myelin sheaths the region motor cortex. Immediately demyelination, cortex neurons...
Significance: Three-photon (3P) microscopy significantly increases the depth and resolution of in vivo imaging due to decreased scattering nonlinear optical sectioning. Simultaneous excitation multiple fluorescent proteins is essential studying multicellular interactions dynamics intact brain. Aim: We characterized laser pulses at a range wavelengths for 3P microscopy, then explored application tdTomato or mScarlet EGFP dual-color single-excitation structural deep living mouse Approach: used...
We mapped the DNase I-hypersensitive sites (DHSS) of serglycin gene in resting and phorbol 12-myristate 13-acetate (PMA)-stimulated human erythroleukemia (HEL) CHRF 288-11 cells, which have megakaryocytic characteristics, HL-60 promyelocytic leukemia cells. compared these DHSS with those normal primary neutrophils umbilical vein endothelial Several appear to be involved regulating level endogenous expression PMA response hematopoietic cell lines. A unique cells induced by may explain high...
Having observed that electrical spinal cord stimulation and training enabled four patients with paraplegia motor complete paralysis to regain voluntary leg movement, the underlying mechanisms involved in forming newly established supraspinal-spinal functional connectivity have become of great interest. van den Brand et al. (Science 336: 1182-1185, 2012) subsequently, demonstrated recovery, response electro-neuromodulation locomotor training, stepping lower limbs rats received a lesion is...
Myelin plasticity occurs when newly-formed and pre-existing oligodendrocytes remodel existing patterns of myelination. Recent studies show these processes occur in response to changes neuronal activity are required for learning memory. However, the link between behaviorally-relevant circuit-specific myelination remains unknown. Using longitudinal, vivo two-photon imaging targeted labeling learning-activated neurons, we explore how pattern intermittent is altered on individual cortical axons...
Myelin loss induces deficits in action potential propagation that result neural dysfunction and contribute to the pathophysiology of neurodegenerative diseases, injury conditions, aging. Because remyelination is often incomplete, better understanding endogenous developing therapies seek restore function are clinical imperatives. Here, we used