A5005618683- Genetics, Aging, and Longevity in Model Organisms
- Animal Ecology and Behavior Studies
- Insect and Pesticide Research
- Insect and Arachnid Ecology and Behavior
- RNA Research and Splicing
- Animal Behavior and Reproduction
- Genomics and Phylogenetic Studies
- Parasite Biology and Host Interactions
- Nematode management and characterization studies
- Chromosomal and Genetic Variations
- CRISPR and Genetic Engineering
- Evolution and Genetic Dynamics
- Insect Resistance and Genetics
- Neuroendocrine regulation and behavior
- RNA modifications and cancer
- Genomics and Chromatin Dynamics
- Plant Molecular Biology Research
- Genetic diversity and population structure
- Physiological and biochemical adaptations
- Plant and animal studies
- Plant Micronutrient Interactions and Effects
Max Planck Institute for Heart and Lung Research
2019-2023
German Centre for Cardiovascular Research
2022
Cardio-Pulmonary Institute
2022
Max Planck Institute for Developmental Biology
2013-2017
Ben-Gurion University of the Negev
2009-2010
Polyphenisms can be adaptations to environments that are heterogeneous in space and time, but persist they require conditional-specific advantages. The nematode Pristionchus pacificus is a facultative predator displays an evolutionarily conserved polyphenism of its mouthparts. During development, P. irreversibly executes either eurystomatous (Eu) or stenostomatous (St) mouth-form, which differ the shape number movable teeth. Eu form, has additional tooth, more complex than St form thus...
SUMMARY The increasing evidence for a role of developmental plasticity in evolution offers exciting prospects testing interactions between ecological and genetic processes. Recent advances with the model organism Pristionchus pacificus have provided inroads to mechanistic understanding plasticity. P. comprises two discontinuous adult mouth‐forms, stenostomatous (“narrow mouthed”) eurystomatous (“wide form, latter which is structurally more complex associated predatory feeding. Both forms are...
Phenotypic plasticity has been suggested to act through developmental switches, but little is known about associated molecular mechanisms. In the nematode Pristionchus pacificus, sulfatase eud-1 was identified as part of a switch controlling mouth-form governing predatory versus bacteriovorous decision. Here we show that mutations in conserved histone-acetyltransferase Ppa-lsy-12 and methyl-binding-protein Ppa-mbd-2 mimic phenotype, resulting absence one mouth-form. Mutations both genes...
Transcriptional adaptation is a recently described phenomenon by which mutation in one gene leads to the transcriptional modulation of related genes, termed adapting genes. At molecular level, it has been proposed that mutant mRNA, rather than loss protein function, activates this response. While several examples have reported zebrafish embryos and mouse cell lines, not known whether observed across metazoans. Here we report C. elegans, find process requires factors involved mRNA decay, as...
Abstract Background The development of multicellular organisms is accompanied by gene expression changes in differentiating cells. Profiling stage-specific during may reveal important insights into sets that contributed to the morphological diversity across animal kingdom. Results We sequenced RNA-seq libraries throughout a developmental timecourse nematode Pristionchus pacificus . transcriptomes reflect early larval stages, adult worms including late larvae, and growth-arrested dauer larvae...
Understanding how new species form requires investigation of evolutionary forces that cause phenotypic and genotypic changes among populations. However, the mechanisms underlying speciation vary little is known about whether genomes diversify in same ways parallel at incipient scale. We address this using nematode, Pristionchus pacificus, which resides an interesting point on continuum (distinct lineages without reproductive isolation), inhabits heterogeneous environments subject to...
Male-biased parasitism is commonly found in higher vertebrates and most likely to be a result of mobility lower immunocompetence male hosts than female hosts. The latter would fitness parasites exploiting males rather females. To test this hypothesis, we investigated foraging reproductive performance fleas (Xenopsylla ramesis) parasitizing Meriones crassus, gerbilline rodent. We allowed feed on groom-restricted rodents predicted that: (1) the size blood meal greater from host (2) will...
SUMMARY The quality of offspring produced by parent fleas (Xenopsylla ramesis) fed on either male or female rodent hosts (Meriones crassus) was studied. emergence success, duration development, resistance to starvation upon and body size the flea were measured. It predicted that parents (i) will survive better as pre-imago, (ii) develop faster, (iii) live longer under after (iv) be larger than hosts. success pre-imaginal relatively high, ranging from 46.9% 100.0% averaging 78.4±3.0%, not...
Transgenerational epigenetic inheritance (TEI) is mostly discussed in the context of physiological or environmental factors. Here, we show intergenerational and transgenerational transcriptional adaptation (TA), a process whereby mutant messenger RNA (mRNA) degradation affects gene expression, nematodes zebrafish. Wild-type offspring animals heterozygous for mRNA-destabilizing alleles display increased expression adapting genes. Notably, nontranscribing do not this response....
Genetic robustness can be achieved via several mechanisms including transcriptional adaptation (TA), a sequence similarity-driven process whereby mutant mRNA degradation products modulate, directly or indirectly, the expression of so-called adapting genes. To identify sequences required for this process, we utilized transgenic approach in Caenorhabditis elegans, combining an overexpression construct gene (act-5) and fluorescent reporter corresponding (act-3). Analyzing series modifications...
The development of multicellular organisms is coordinated by various gene regulatory mechanisms that ensure correct spatio-temporal patterns expression. Recently, the role antisense transcription in regulation has moved into focus research. To characterize genome-wide and to study their evolutionary conservation, we sequenced a strand-specific RNA-seq library nematode Pristionchus pacificus. We identified 1112 configurations which largest group represents 465 transcripts (ASTs) are fully...