A5052882915- Biofuel production and bioconversion
- Enzyme Production and Characterization
- Genomics and Phylogenetic Studies
- Studies on Chitinases and Chitosanases
- Advanced Cellulose Research Studies
- Enzyme Catalysis and Immobilization
- Bacteriophages and microbial interactions
- Microbial Metabolic Engineering and Bioproduction
- Legume Nitrogen Fixing Symbiosis
- Polysaccharides and Plant Cell Walls
- Catalysis for Biomass Conversion
- Plant-Microbe Interactions and Immunity
- Essential Oils and Antimicrobial Activity
- Diet, Metabolism, and Disease
- Identification and Quantification in Food
- Microbial Metabolites in Food Biotechnology
- Plant Pathogens and Fungal Diseases
- Coconut Research and Applications
- Genomics and Chromatin Dynamics
- GABA and Rice Research
- Enzyme Structure and Function
- Plant Gene Expression Analysis
- Fungal and yeast genetics research
- Anaerobic Digestion and Biogas Production
- Biopolymer Synthesis and Applications
King Mongkut's University of Technology Thonburi
2016-2025
Japan International Research Center for Agricultural Sciences
2020-2024
Rice straw (RS) is an abundant, readily available agricultural waste, which shows promise as a potential feedstock for Asian ethanol production. To enhance release of glucose by enzymatic hydrolysis, RS was pretreated with aqueous ammonia (27% w/w) at two pretreatment temperatures: room temperature and 60°C. Statistical analysis indicated similarity production both temperatures after 3-day incubation. Chemical composition, FTIR, EDX analyses confirmed the retention glucan xylan in solid, but...
Maximizing saccharification efficiency of lignocellulose and minimizing the production costs associated with enzyme requirements are crucial for sustainable biofuel production. This study presents a novel semi-fed-batch method that uses co-culture Clostridium thermocellum Thermobrachium celere strain A9 to efficiently break down high solid-loading lignocellulosic biomass without need any external enzymes. optimizes enhances glucose from alkaline-treated rice straw, representative biomass....
Complete utilization of carbohydrate fractions is one the prerequisites for obtaining economically favorable lignocellulosic biomass conversion. This study shows that xylan in untreated rice straw was saccharified to xylose step without chemical pretreatment, yielding 58.2% theoretically maximum value by Paenibacillus curdlanolyticus B-6 PcAxy43A, a weak lignin-binding trifunctional xylanolytic enzyme, endoxylanase/β-xylosidase/arabinoxylan arabinofuranohydrolase. Moreover, yield from...
D-Psicose is a rare, low-calorie sugar that found in limited quantities national products. Recently, D-psicose has gained considerable attention due to its potential applications the food, nutraceutical, and pharmaceutical industries. In this study, novel 3-epimerase (a group of ketose 3-epimerase) from an extremely halophilic, anaerobic bacterium, Iocasia fonsfrigidae strain SP3-1 (IfDPEase), was cloned, expressed Escherichia coli, characterized. Unlike other members, IfDPEase shows...
The axy43A gene encoding the intracellular trifunctional xylanolytic enzyme from Paenibacillus curdlanolyticus B-6 was cloned and expressed in Escherichia coli Recombinant PcAxy43A consisting of a glycoside hydrolase family 43 6 carbohydrate-binding module exhibited endo-xylanase, β-xylosidase, arabinoxylan arabinofuranohydrolase activities. hydrolyzed xylohexaose birch wood xylan to release series xylooligosaccharides, indicating that contained endo-xylanase activity. β-xylosidase activity...
An anaerobic thermophilic bacterial strain, A9 (NITE P-03545), that secretes β-glucosidase was newly isolated from wastewater sediments by screening using esculin. The 16S rRNA gene sequence of strain had 100% identity with Thermobrachium celere type JW/YL-NZ35. complete genome showed 98.4% average nucleotide However, different physiological properties JW/YL-NZ35, which cannot secrete β-glucosidases or grow on cellobiose as the sole carbon source. key (TcBG1) A9, belongs to glycoside...
A novel Gram-negative, spore forming, obligately anaerobic, thermophilic, chitin-degrading bacterium, designated UUS1-1T, was isolated from compost on Ishigaki Island, Japan by enrichment culturing using chitin powder as the carbon source. The strain has unique, long, hair-like rod morphological features and exhibits strong degradation activity toward crystalline under thermophilic conditions. Growth of observed at 45-65 °C (optimum, 55 °C) pH 6.5-7.5 7.0). In addition to chitin, utilized...
Thermophilic, facultatively anaerobic, xylanolytic bacterial strain DA-C8 (=JCM34211 =DSM111723), newly isolated from compost, shows strong beechwood xylan degradation ability. Whole-genome sequencing of on the Ion GeneStudio S5 system yielded 69 contigs with a total size 3,110,565 bp, 2,877 protein-coding sequences, and G+C content 52.3 mol%. Genome annotation revealed that possesses debranching enzymes, such as β-L-arabinofuranosidase polygalacturonase, are important for efficient xylan....
Strain UUS1-1 (=JCM33882 =DSM111537) is a novel chitinolytic, thermophilic, anaerobic bacterium belonging to the genus Hydrogenispora of uncultured taxonomic OPB54 cluster within phylum Firmicutes. has unique, long, hair-like rod morphology and strong ability degrade crystalline chitin. The whole genome strain was sequenced on an Ion GeneStudio S5 system, which yielded 86 contigs comprising 2,482,547 bp, 2235 protein-coding sequences, G+C content 52.1 mol%. second cultivable isolate, besides...
We isolated and analysed a Gram-negative, facultatively thermophilic, xylan-degrading bacterium that we designated as strain DA-C8T. The was from compost Ishigaki Island, Japan, by enrichment culturing using beech wood xylan the sole carbon source. showed high degradation ability under anaerobic growth conditions. isolate grew at 37-60 °C (optimum, 55 °C) pH 4.0-11.0 9.0). As well xylan, DA-C8T could use polysaccharides such arabinoxylan galactan sources. Comparison of 16S rRNA gene...
Paenibacillus curdlanolyticus B-6 is a facultative anaerobic bacterium that efficiently produces lignocellulolytic multienzyme complex. The whole genome of P. was sequenced on an Ion GeneStudio S5 system, which yielded 74 contigs with total size 4,875,097 bp, 4,473 protein-coding sequences, and G+C content 49.7%. data have been deposited in DDBJ/ENA/GenBank under accession numbers BLWM01000001–BLWM01000074. Analyses average nucleotide identities phylogenetic relationships 16S rRNA sequences...
Abstract Paenibacillus curdlanolyticus B-6 produces an extracellular multienzyme complex containing a hypothetical scaffolding-like protein and several xylanases cellulases. The largest (280-kDa) component protein, called S1, has cellulose-binding ability xylanase activity, thus was considered to function like the scaffolding proteins found in cellulosomes. S1 consists of 863 amino acid residues with predicted molecular mass 91,029 Da includes two N-terminal surface layer homology (SLH)...
To discover more efficient degradation processes of lignocellulosic biomass, it is still important to analyze genomic and enzymatic data from bacteria that have strong xylanolytic ability. Here, we present the draft genome sequences Paenibacillus cisolokensis strain LC2-13A Xylanibacillus composti K-13 are closest sp. DA-C8, which has xylan ability under anaerobic growth conditions. Whole-genome sequencing on Ion GeneStudio S5 System yielded 277 contigs with total size 5,305,208 bp G+C...